Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23220 and BPHYT_RS23225 are separated by 113 nucleotides BPHYT_RS23225 and BPHYT_RS23230 are separated by 30 nucleotides BPHYT_RS23230 and BPHYT_RS23235 are separated by 185 nucleotides BPHYT_RS23235 and BPHYT_RS23240 are separated by 350 nucleotides
BPHYT_RS23220: BPHYT_RS23220 - hypothetical protein, at 774,648 to 775,379
_RS23220
BPHYT_RS23225: BPHYT_RS23225 - hypothetical protein, at 775,493 to 775,699
_RS23225
BPHYT_RS23230: BPHYT_RS23230 - histidine kinase, at 775,730 to 777,703
_RS23230
BPHYT_RS23235: BPHYT_RS23235 - hypothetical protein, at 777,889 to 778,272
_RS23235
BPHYT_RS23240: BPHYT_RS23240 - DNA-binding protein, at 778,623 to 778,901
_RS23240
Position (kb) 775
776
777
778 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 774.741 kb on + strand, within BPHYT_RS23220 at 775.111 kb on - strand, within BPHYT_RS23220 at 775.313 kb on + strand at 775.608 kb on - strand, within BPHYT_RS23225 at 775.610 kb on + strand, within BPHYT_RS23225 at 775.752 kb on + strand at 776.257 kb on - strand, within BPHYT_RS23230 at 777.315 kb on + strand, within BPHYT_RS23230 at 777.714 kb on + strand at 777.714 kb on + strand at 777.784 kb on - strand at 778.296 kb on + strand at 778.301 kb on - strand at 778.323 kb on - strand at 778.345 kb on + strand at 778.431 kb on + strand at 778.431 kb on + strand at 778.467 kb on - strand at 778.514 kb on + strand at 778.514 kb on + strand at 778.515 kb on - strand at 778.523 kb on + strand at 778.559 kb on + strand at 778.605 kb on + strand at 778.611 kb on - strand at 778.622 kb on + strand at 778.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 774,741 + BPHYT_RS23220 0.13 +0.4 775,111 - BPHYT_RS23220 0.63 -0.3 775,313 + -0.4 775,608 - BPHYT_RS23225 0.56 +1.7 775,610 + BPHYT_RS23225 0.57 -1.6 775,752 + +5.2 776,257 - BPHYT_RS23230 0.27 -2.7 777,315 + BPHYT_RS23230 0.80 +2.3 777,714 + +0.8 777,714 + +0.4 777,784 - +1.3 778,296 + -1.5 778,301 - -1.0 778,323 - +0.5 778,345 + -0.9 778,431 + +1.9 778,431 + +0.5 778,467 - +1.5 778,514 + +0.3 778,514 + +1.0 778,515 - -0.3 778,523 + -0.1 778,559 + -0.4 778,605 + +0.4 778,611 - +0.0 778,622 + -0.4 778,623 - +0.1
Or see this region's nucleotide sequence