Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23075 and BPHYT_RS23080 are separated by 225 nucleotides BPHYT_RS23080 and BPHYT_RS23085 are separated by 48 nucleotides
BPHYT_RS23075: BPHYT_RS23075 - GntR family transcriptional regulator, at 740,374 to 741,030
_RS23075
BPHYT_RS23080: BPHYT_RS23080 - gamma-glutamyltransferase, at 741,256 to 743,040
_RS23080
BPHYT_RS23085: BPHYT_RS23085 - MFS transporter, at 743,089 to 744,501
_RS23085
Position (kb) 741
742
743
744 Strain fitness (log2 ratio) -2
-1
0
1
2 at 740.267 kb on - strand at 740.267 kb on - strand at 740.295 kb on + strand at 740.844 kb on - strand, within BPHYT_RS23075 at 741.201 kb on + strand at 741.540 kb on - strand, within BPHYT_RS23080 at 741.658 kb on + strand, within BPHYT_RS23080 at 741.835 kb on + strand, within BPHYT_RS23080 at 741.837 kb on + strand, within BPHYT_RS23080 at 741.837 kb on + strand, within BPHYT_RS23080 at 741.838 kb on - strand, within BPHYT_RS23080 at 741.838 kb on - strand, within BPHYT_RS23080 at 742.182 kb on + strand, within BPHYT_RS23080 at 743.231 kb on + strand, within BPHYT_RS23085 at 743.278 kb on + strand, within BPHYT_RS23085 at 743.279 kb on - strand, within BPHYT_RS23085 at 743.368 kb on + strand, within BPHYT_RS23085 at 743.555 kb on - strand, within BPHYT_RS23085 at 743.847 kb on + strand, within BPHYT_RS23085 at 743.924 kb on - strand, within BPHYT_RS23085 at 744.029 kb on - strand, within BPHYT_RS23085
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 740,267 - +2.4 740,267 - -2.0 740,295 + +0.6 740,844 - BPHYT_RS23075 0.72 +0.6 741,201 + +0.6 741,540 - BPHYT_RS23080 0.16 +1.4 741,658 + BPHYT_RS23080 0.23 -0.3 741,835 + BPHYT_RS23080 0.32 +0.9 741,837 + BPHYT_RS23080 0.33 -2.7 741,837 + BPHYT_RS23080 0.33 +0.7 741,838 - BPHYT_RS23080 0.33 +0.0 741,838 - BPHYT_RS23080 0.33 +0.5 742,182 + BPHYT_RS23080 0.52 -2.2 743,231 + BPHYT_RS23085 0.10 -0.5 743,278 + BPHYT_RS23085 0.13 -0.5 743,279 - BPHYT_RS23085 0.13 -1.2 743,368 + BPHYT_RS23085 0.20 -2.7 743,555 - BPHYT_RS23085 0.33 +2.0 743,847 + BPHYT_RS23085 0.54 +1.1 743,924 - BPHYT_RS23085 0.59 +2.4 744,029 - BPHYT_RS23085 0.67 +0.7
Or see this region's nucleotide sequence