Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22940 and BPHYT_RS22945 are separated by 1 nucleotides BPHYT_RS22945 and BPHYT_RS22950 are separated by 53 nucleotides BPHYT_RS22950 and BPHYT_RS22955 are separated by 7 nucleotides BPHYT_RS22955 and BPHYT_RS22960 are separated by 63 nucleotides
BPHYT_RS22940: BPHYT_RS22940 - pilus assembly protein TadE, at 705,252 to 705,782
_RS22940
BPHYT_RS22945: BPHYT_RS22945 - pilus assembly protein TadE, at 705,784 to 706,413
_RS22945
BPHYT_RS22950: BPHYT_RS22950 - von Willebrand factor A, at 706,467 to 707,822
_RS22950
BPHYT_RS22955: BPHYT_RS22955 - isoprenylcysteine carboxyl methyltransferase, at 707,830 to 708,543
_RS22955
BPHYT_RS22960: BPHYT_RS22960 - peptidase, at 708,607 to 710,145
_RS22960
Position (kb) 706
707
708 Strain fitness (log2 ratio) -2
-1
0
1
2 at 705.514 kb on - strand, within BPHYT_RS22940 at 705.630 kb on + strand, within BPHYT_RS22940 at 705.630 kb on + strand, within BPHYT_RS22940 at 705.887 kb on - strand, within BPHYT_RS22945 at 706.051 kb on + strand, within BPHYT_RS22945 at 706.052 kb on - strand, within BPHYT_RS22945 at 706.322 kb on + strand, within BPHYT_RS22945 at 706.393 kb on + strand at 706.599 kb on - strand at 706.934 kb on + strand, within BPHYT_RS22950 at 707.069 kb on - strand, within BPHYT_RS22950 at 707.130 kb on + strand, within BPHYT_RS22950 at 707.256 kb on - strand, within BPHYT_RS22950 at 707.353 kb on + strand, within BPHYT_RS22950 at 707.353 kb on + strand, within BPHYT_RS22950 at 707.526 kb on + strand, within BPHYT_RS22950 at 707.527 kb on - strand, within BPHYT_RS22950 at 707.527 kb on - strand, within BPHYT_RS22950 at 707.743 kb on - strand at 707.811 kb on - strand at 708.161 kb on - strand, within BPHYT_RS22955 at 708.325 kb on + strand, within BPHYT_RS22955 at 708.359 kb on - strand, within BPHYT_RS22955 at 708.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 705,514 - BPHYT_RS22940 0.49 -0.0 705,630 + BPHYT_RS22940 0.71 -0.5 705,630 + BPHYT_RS22940 0.71 -0.8 705,887 - BPHYT_RS22945 0.16 +0.4 706,051 + BPHYT_RS22945 0.42 +1.3 706,052 - BPHYT_RS22945 0.43 +0.3 706,322 + BPHYT_RS22945 0.85 +0.1 706,393 + +0.1 706,599 - -0.0 706,934 + BPHYT_RS22950 0.34 +0.3 707,069 - BPHYT_RS22950 0.44 +1.1 707,130 + BPHYT_RS22950 0.49 +1.2 707,256 - BPHYT_RS22950 0.58 -0.5 707,353 + BPHYT_RS22950 0.65 -2.7 707,353 + BPHYT_RS22950 0.65 -0.4 707,526 + BPHYT_RS22950 0.78 +1.3 707,527 - BPHYT_RS22950 0.78 -1.3 707,527 - BPHYT_RS22950 0.78 -0.0 707,743 - -0.5 707,811 - +0.3 708,161 - BPHYT_RS22955 0.46 +0.5 708,325 + BPHYT_RS22955 0.69 +2.1 708,359 - BPHYT_RS22955 0.74 -1.2 708,548 - +1.0
Or see this region's nucleotide sequence