Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22845 and BPHYT_RS22850 are separated by 213 nucleotides BPHYT_RS22850 and BPHYT_RS22855 are separated by 210 nucleotides BPHYT_RS22855 and BPHYT_RS22860 are separated by 126 nucleotides BPHYT_RS22860 and BPHYT_RS22865 overlap by 4 nucleotides
BPHYT_RS22845: BPHYT_RS22845 - hypothetical protein, at 685,061 to 685,309
_RS22845
BPHYT_RS22850: BPHYT_RS22850 - thioredoxin, at 685,523 to 686,089
_RS22850
BPHYT_RS22855: BPHYT_RS22855 - aldehyde-activating protein, at 686,300 to 686,671
_RS22855
BPHYT_RS22860: BPHYT_RS22860 - chemotaxis protein CheY, at 686,798 to 687,538
_RS22860
BPHYT_RS22865: BPHYT_RS22865 - ATPase, at 687,535 to 688,860
_RS22865
Position (kb) 686
687 Strain fitness (log2 ratio) -2
-1
0
1 at 685.597 kb on - strand, within BPHYT_RS22850 at 685.653 kb on + strand, within BPHYT_RS22850 at 686.152 kb on - strand at 686.152 kb on - strand at 686.557 kb on + strand, within BPHYT_RS22855 at 686.612 kb on + strand, within BPHYT_RS22855 at 686.613 kb on - strand, within BPHYT_RS22855 at 686.615 kb on - strand, within BPHYT_RS22855 at 686.817 kb on + strand at 687.407 kb on - strand, within BPHYT_RS22860
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 685,597 - BPHYT_RS22850 0.13 -0.6 685,653 + BPHYT_RS22850 0.23 -0.1 686,152 - -0.4 686,152 - +1.3 686,557 + BPHYT_RS22855 0.69 +1.4 686,612 + BPHYT_RS22855 0.84 -1.8 686,613 - BPHYT_RS22855 0.84 -0.3 686,615 - BPHYT_RS22855 0.85 -1.0 686,817 + +0.7 687,407 - BPHYT_RS22860 0.82 +0.3
Or see this region's nucleotide sequence