Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22715 and BPHYT_RS22720 are separated by 178 nucleotides BPHYT_RS22720 and BPHYT_RS22725 are separated by 195 nucleotides
BPHYT_RS22715: BPHYT_RS22715 - L-2-keto-3-deoxyarabonate dehydratase (EC 4.2.1.43) <small>(from data)</small>, at 651,362 to 652,294
_RS22715
BPHYT_RS22720: BPHYT_RS22720 - LysR family transcriptional regulator, at 652,473 to 653,456
_RS22720
BPHYT_RS22725: BPHYT_RS22725 - glycosyl transferase family 51, at 653,652 to 656,732
_RS22725
Position (kb) 652
653
654 Strain fitness (log2 ratio) -1
0
1
2
3 at 651.598 kb on - strand, within BPHYT_RS22715 at 651.598 kb on - strand, within BPHYT_RS22715 at 651.916 kb on - strand, within BPHYT_RS22715 at 651.916 kb on - strand, within BPHYT_RS22715 at 652.024 kb on - strand, within BPHYT_RS22715 at 652.408 kb on + strand at 652.411 kb on - strand at 652.411 kb on - strand at 652.760 kb on - strand, within BPHYT_RS22720 at 652.974 kb on + strand, within BPHYT_RS22720 at 652.975 kb on - strand, within BPHYT_RS22720 at 652.981 kb on - strand, within BPHYT_RS22720 at 653.091 kb on + strand, within BPHYT_RS22720 at 653.593 kb on - strand at 653.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 651,598 - BPHYT_RS22715 0.25 +0.1 651,598 - BPHYT_RS22715 0.25 -0.7 651,916 - BPHYT_RS22715 0.59 -1.4 651,916 - BPHYT_RS22715 0.59 +3.5 652,024 - BPHYT_RS22715 0.71 -0.1 652,408 + +1.2 652,411 - -0.3 652,411 - -0.2 652,760 - BPHYT_RS22720 0.29 +1.0 652,974 + BPHYT_RS22720 0.51 -0.1 652,975 - BPHYT_RS22720 0.51 -1.0 652,981 - BPHYT_RS22720 0.52 +0.4 653,091 + BPHYT_RS22720 0.63 -0.5 653,593 - +1.6 653,812 - -0.1
Or see this region's nucleotide sequence