Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22700 and BPHYT_RS22705 are separated by 131 nucleotides BPHYT_RS22705 and BPHYT_RS22710 overlap by 1 nucleotides BPHYT_RS22710 and BPHYT_RS22715 are separated by 442 nucleotides
BPHYT_RS22700: BPHYT_RS22700 - FAD-dependent oxidoreductase, at 648,156 to 649,436
_RS22700
BPHYT_RS22705: BPHYT_RS22705 - hypothetical protein, at 649,568 to 650,350
_RS22705
BPHYT_RS22710: BPHYT_RS22710 - XRE family transcriptional regulator, at 650,350 to 650,919
_RS22710
BPHYT_RS22715: BPHYT_RS22715 - L-2-keto-3-deoxyarabonate dehydratase (EC 4.2.1.43) (from data) , at 651,362 to 652,294
_RS22715
Position (kb)
650
651 Strain fitness (log2 ratio)
-1
0
1
2
3 at 650.302 kb on - strand at 650.334 kb on + strand at 650.410 kb on - strand, within BPHYT_RS22710 at 650.449 kb on - strand, within BPHYT_RS22710 at 650.784 kb on + strand, within BPHYT_RS22710 at 650.825 kb on + strand, within BPHYT_RS22710 at 650.948 kb on + strand at 650.949 kb on - strand at 650.954 kb on - strand at 650.976 kb on + strand at 650.976 kb on + strand at 651.081 kb on - strand at 651.198 kb on - strand at 651.198 kb on - strand at 651.198 kb on - strand at 651.363 kb on + strand at 651.364 kb on - strand at 651.598 kb on - strand, within BPHYT_RS22715 at 651.598 kb on - strand, within BPHYT_RS22715 at 651.916 kb on - strand, within BPHYT_RS22715 at 651.916 kb on - strand, within BPHYT_RS22715
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 650,302 - +0.2 650,334 + +0.5 650,410 - BPHYT_RS22710 0.11 +1.2 650,449 - BPHYT_RS22710 0.17 -0.3 650,784 + BPHYT_RS22710 0.76 -0.8 650,825 + BPHYT_RS22710 0.83 +3.5 650,948 + -0.4 650,949 - -0.4 650,954 - -0.6 650,976 + +0.2 650,976 + +0.8 651,081 - -1.7 651,198 - +0.3 651,198 - -0.3 651,198 - -0.0 651,363 + +2.8 651,364 - -1.4 651,598 - BPHYT_RS22715 0.25 +0.1 651,598 - BPHYT_RS22715 0.25 -0.7 651,916 - BPHYT_RS22715 0.59 -1.4 651,916 - BPHYT_RS22715 0.59 +3.5
Or see this region's nucleotide sequence