Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22690 and BPHYT_RS22695 are separated by 70 nucleotides BPHYT_RS22695 and BPHYT_RS22700 are separated by 94 nucleotides BPHYT_RS22700 and BPHYT_RS22705 are separated by 131 nucleotides BPHYT_RS22705 and BPHYT_RS22710 overlap by 1 nucleotides
BPHYT_RS22690: BPHYT_RS22690 - D-galactonate transporter, at 645,919 to 647,295
_RS22690
BPHYT_RS22695: BPHYT_RS22695 - TetR family transcriptional regulator, at 647,366 to 648,061
_RS22695
BPHYT_RS22700: BPHYT_RS22700 - FAD-dependent oxidoreductase, at 648,156 to 649,436
_RS22700
BPHYT_RS22705: BPHYT_RS22705 - hypothetical protein, at 649,568 to 650,350
_RS22705
BPHYT_RS22710: BPHYT_RS22710 - XRE family transcriptional regulator, at 650,350 to 650,919
_RS22710
Position (kb) 648
649
650 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 647.473 kb on + strand, within BPHYT_RS22695 at 647.474 kb on - strand, within BPHYT_RS22695 at 647.594 kb on - strand, within BPHYT_RS22695 at 647.644 kb on - strand, within BPHYT_RS22695 at 647.717 kb on - strand, within BPHYT_RS22695 at 647.781 kb on - strand, within BPHYT_RS22695 at 647.799 kb on + strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.847 kb on + strand, within BPHYT_RS22695 at 647.848 kb on - strand, within BPHYT_RS22695 at 647.853 kb on + strand, within BPHYT_RS22695 at 647.853 kb on + strand, within BPHYT_RS22695 at 647.854 kb on - strand, within BPHYT_RS22695 at 647.980 kb on - strand, within BPHYT_RS22695 at 647.980 kb on - strand, within BPHYT_RS22695 at 648.094 kb on - strand at 648.478 kb on + strand, within BPHYT_RS22700 at 648.479 kb on - strand, within BPHYT_RS22700 at 648.590 kb on - strand, within BPHYT_RS22700 at 648.590 kb on - strand, within BPHYT_RS22700 at 648.590 kb on - strand, within BPHYT_RS22700 at 648.613 kb on + strand, within BPHYT_RS22700 at 648.613 kb on + strand, within BPHYT_RS22700 at 648.679 kb on + strand, within BPHYT_RS22700 at 648.890 kb on - strand, within BPHYT_RS22700 at 649.157 kb on - strand, within BPHYT_RS22700 at 649.215 kb on - strand, within BPHYT_RS22700 at 650.302 kb on - strand at 650.334 kb on + strand at 650.410 kb on - strand, within BPHYT_RS22710
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 647,473 + BPHYT_RS22695 0.15 -0.3 647,474 - BPHYT_RS22695 0.16 +0.3 647,594 - BPHYT_RS22695 0.33 -1.5 647,644 - BPHYT_RS22695 0.40 +0.9 647,717 - BPHYT_RS22695 0.50 -1.2 647,781 - BPHYT_RS22695 0.60 +0.0 647,799 + BPHYT_RS22695 0.62 -0.7 647,800 - BPHYT_RS22695 0.62 +0.6 647,800 - BPHYT_RS22695 0.62 -1.5 647,800 - BPHYT_RS22695 0.62 +0.2 647,800 - BPHYT_RS22695 0.62 -2.3 647,847 + BPHYT_RS22695 0.69 -0.9 647,848 - BPHYT_RS22695 0.69 -0.3 647,853 + BPHYT_RS22695 0.70 +0.2 647,853 + BPHYT_RS22695 0.70 -0.4 647,854 - BPHYT_RS22695 0.70 +2.8 647,980 - BPHYT_RS22695 0.88 -1.2 647,980 - BPHYT_RS22695 0.88 -1.7 648,094 - -0.4 648,478 + BPHYT_RS22700 0.25 +0.1 648,479 - BPHYT_RS22700 0.25 -0.1 648,590 - BPHYT_RS22700 0.34 -0.0 648,590 - BPHYT_RS22700 0.34 +0.6 648,590 - BPHYT_RS22700 0.34 -0.6 648,613 + BPHYT_RS22700 0.36 -0.3 648,613 + BPHYT_RS22700 0.36 -2.0 648,679 + BPHYT_RS22700 0.41 -0.2 648,890 - BPHYT_RS22700 0.57 +0.0 649,157 - BPHYT_RS22700 0.78 +0.7 649,215 - BPHYT_RS22700 0.83 -0.0 650,302 - +0.2 650,334 + +0.5 650,410 - BPHYT_RS22710 0.11 +1.2
Or see this region's nucleotide sequence