Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22640 and BPHYT_RS22645 overlap by 4 nucleotides BPHYT_RS22645 and BPHYT_RS22650 are separated by 481 nucleotides
BPHYT_RS22640: BPHYT_RS22640 - hydroxyproline-2-epimerase, at 635,888 to 636,844
_RS22640
BPHYT_RS22645: BPHYT_RS22645 - D-amino acid oxidase, at 636,841 to 637,968
_RS22645
BPHYT_RS22650: BPHYT_RS22650 - pyridine nucleotide-disulfide oxidoreductase, at 638,450 to 639,736
_RS22650
Position (kb) 636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 635.911 kb on + strand at 635.912 kb on - strand at 636.593 kb on + strand, within BPHYT_RS22640 at 636.719 kb on + strand, within BPHYT_RS22640 at 636.773 kb on + strand at 636.773 kb on + strand at 637.027 kb on + strand, within BPHYT_RS22645 at 637.273 kb on + strand, within BPHYT_RS22645 at 637.273 kb on + strand, within BPHYT_RS22645 at 637.416 kb on - strand, within BPHYT_RS22645 at 637.416 kb on - strand, within BPHYT_RS22645 at 637.416 kb on - strand, within BPHYT_RS22645 at 637.543 kb on + strand, within BPHYT_RS22645 at 637.705 kb on + strand, within BPHYT_RS22645 at 637.705 kb on + strand, within BPHYT_RS22645 at 637.706 kb on - strand, within BPHYT_RS22645 at 638.015 kb on - strand at 638.113 kb on - strand at 638.238 kb on + strand at 638.239 kb on - strand at 638.253 kb on + strand at 638.253 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 635,911 + -1.2 635,912 - -0.1 636,593 + BPHYT_RS22640 0.74 -0.9 636,719 + BPHYT_RS22640 0.87 -1.3 636,773 + -1.3 636,773 + -2.8 637,027 + BPHYT_RS22645 0.16 -1.2 637,273 + BPHYT_RS22645 0.38 +0.3 637,273 + BPHYT_RS22645 0.38 -1.1 637,416 - BPHYT_RS22645 0.51 -0.6 637,416 - BPHYT_RS22645 0.51 -0.2 637,416 - BPHYT_RS22645 0.51 -2.8 637,543 + BPHYT_RS22645 0.62 -0.1 637,705 + BPHYT_RS22645 0.77 -0.0 637,705 + BPHYT_RS22645 0.77 -0.4 637,706 - BPHYT_RS22645 0.77 +0.7 638,015 - +1.0 638,113 - -1.8 638,238 + -1.2 638,239 - +0.8 638,253 + -0.5 638,253 + -0.7
Or see this region's nucleotide sequence