Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22625

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22620 and BPHYT_RS22625 are separated by 156 nucleotidesBPHYT_RS22625 and BPHYT_RS22630 are separated by 398 nucleotides BPHYT_RS22620: BPHYT_RS22620 - MFS transporter, at 630,539 to 632,014 _RS22620 BPHYT_RS22625: BPHYT_RS22625 - MFS transporter, at 632,171 to 633,472 _RS22625 BPHYT_RS22630: BPHYT_RS22630 - transcriptional regulator, at 633,871 to 634,611 _RS22630 Position (kb) 632 633 634Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 631.289 kb on + strand, within BPHYT_RS22620at 631.290 kb on - strand, within BPHYT_RS22620at 631.290 kb on - strand, within BPHYT_RS22620at 631.752 kb on - strand, within BPHYT_RS22620at 631.843 kb on - strand, within BPHYT_RS22620at 631.925 kb on + strandat 631.926 kb on - strandat 632.026 kb on + strandat 632.027 kb on - strandat 632.027 kb on - strandat 632.241 kb on + strandat 632.241 kb on + strandat 632.242 kb on - strandat 632.242 kb on - strandat 632.317 kb on - strand, within BPHYT_RS22625at 632.431 kb on - strand, within BPHYT_RS22625at 632.481 kb on + strand, within BPHYT_RS22625at 632.481 kb on + strand, within BPHYT_RS22625at 632.482 kb on - strand, within BPHYT_RS22625at 632.482 kb on - strand, within BPHYT_RS22625at 632.482 kb on - strand, within BPHYT_RS22625at 632.485 kb on - strand, within BPHYT_RS22625at 632.485 kb on - strand, within BPHYT_RS22625at 632.830 kb on - strand, within BPHYT_RS22625at 632.988 kb on + strand, within BPHYT_RS22625at 632.989 kb on - strand, within BPHYT_RS22625at 633.120 kb on + strand, within BPHYT_RS22625at 633.120 kb on + strand, within BPHYT_RS22625at 633.317 kb on + strand, within BPHYT_RS22625at 633.317 kb on + strand, within BPHYT_RS22625at 633.318 kb on - strand, within BPHYT_RS22625at 633.318 kb on - strand, within BPHYT_RS22625at 633.507 kb on + strandat 633.508 kb on - strandat 633.508 kb on - strandat 633.520 kb on + strandat 633.664 kb on + strandat 633.665 kb on - strandat 633.751 kb on + strandat 633.791 kb on - strandat 633.794 kb on + strandat 633.795 kb on - strandat 633.800 kb on - strandat 633.853 kb on + strandat 633.858 kb on + strandat 633.859 kb on - strandat 633.859 kb on - strandat 633.876 kb on + strandat 633.876 kb on + strandat 633.959 kb on - strand, within BPHYT_RS22630at 634.165 kb on + strand, within BPHYT_RS22630at 634.165 kb on + strand, within BPHYT_RS22630at 634.232 kb on - strand, within BPHYT_RS22630

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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631,289 + BPHYT_RS22620 0.51 +0.4
631,290 - BPHYT_RS22620 0.51 +0.3
631,290 - BPHYT_RS22620 0.51 +0.7
631,752 - BPHYT_RS22620 0.82 -0.8
631,843 - BPHYT_RS22620 0.88 +0.2
631,925 + +2.3
631,926 - +1.0
632,026 + -1.2
632,027 - -1.3
632,027 - +0.7
632,241 + -1.9
632,241 + +1.9
632,242 - +0.9
632,242 - -1.8
632,317 - BPHYT_RS22625 0.11 -1.5
632,431 - BPHYT_RS22625 0.20 -1.1
632,481 + BPHYT_RS22625 0.24 +3.3
632,481 + BPHYT_RS22625 0.24 -2.2
632,482 - BPHYT_RS22625 0.24 +0.3
632,482 - BPHYT_RS22625 0.24 +0.0
632,482 - BPHYT_RS22625 0.24 +1.7
632,485 - BPHYT_RS22625 0.24 -0.6
632,485 - BPHYT_RS22625 0.24 -0.6
632,830 - BPHYT_RS22625 0.51 +0.2
632,988 + BPHYT_RS22625 0.63 +0.4
632,989 - BPHYT_RS22625 0.63 -0.8
633,120 + BPHYT_RS22625 0.73 -0.2
633,120 + BPHYT_RS22625 0.73 -1.4
633,317 + BPHYT_RS22625 0.88 +0.4
633,317 + BPHYT_RS22625 0.88 +0.3
633,318 - BPHYT_RS22625 0.88 -0.4
633,318 - BPHYT_RS22625 0.88 +1.1
633,507 + -0.8
633,508 - -0.4
633,508 - -0.2
633,520 + +1.3
633,664 + +1.7
633,665 - -1.0
633,751 + -3.0
633,791 - -1.2
633,794 + +1.2
633,795 - -0.1
633,800 - -1.5
633,853 + -0.5
633,858 + +0.3
633,859 - +0.1
633,859 - -2.6
633,876 + +0.3
633,876 + -2.7
633,959 - BPHYT_RS22630 0.12 -0.6
634,165 + BPHYT_RS22630 0.40 +1.8
634,165 + BPHYT_RS22630 0.40 +1.3
634,232 - BPHYT_RS22630 0.49 -0.5

Or see this region's nucleotide sequence