Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22235 and BPHYT_RS22240 are separated by 256 nucleotides BPHYT_RS22240 and BPHYT_RS22245 overlap by 4 nucleotides BPHYT_RS22245 and BPHYT_RS22250 are separated by 74 nucleotides
BPHYT_RS22235: BPHYT_RS22235 - sensor histidine kinase, at 546,243 to 547,658
_RS22235
BPHYT_RS22240: BPHYT_RS22240 - hypothetical protein, at 547,915 to 548,112
_RS22240
BPHYT_RS22245: BPHYT_RS22245 - transporter for D-alanine, D-lactate, and pyruvate (MctP) <small>(from data)</small>, at 548,109 to 549,590
_RS22245
BPHYT_RS22250: BPHYT_RS22250 - D-amino acid dehydrogenase, at 549,665 to 550,909
_RS22250
Position (kb) 548
549
550 Strain fitness (log2 ratio) -1
0
1
2 at 547.112 kb on - strand, within BPHYT_RS22235 at 547.217 kb on - strand, within BPHYT_RS22235 at 547.217 kb on - strand, within BPHYT_RS22235 at 547.523 kb on + strand at 547.524 kb on - strand at 547.525 kb on + strand at 547.526 kb on - strand at 547.687 kb on - strand at 547.690 kb on - strand at 547.692 kb on + strand at 547.693 kb on - strand at 547.710 kb on - strand at 547.745 kb on + strand at 547.746 kb on - strand at 547.814 kb on - strand at 547.816 kb on + strand at 547.816 kb on + strand at 547.816 kb on + strand at 547.816 kb on + strand at 547.817 kb on - strand at 547.972 kb on + strand, within BPHYT_RS22240 at 547.975 kb on - strand, within BPHYT_RS22240 at 547.975 kb on - strand, within BPHYT_RS22240 at 548.042 kb on - strand, within BPHYT_RS22240 at 548.086 kb on + strand, within BPHYT_RS22240 at 548.087 kb on - strand, within BPHYT_RS22240 at 548.298 kb on + strand, within BPHYT_RS22245 at 548.307 kb on + strand, within BPHYT_RS22245 at 548.338 kb on - strand, within BPHYT_RS22245 at 548.538 kb on + strand, within BPHYT_RS22245 at 548.539 kb on - strand, within BPHYT_RS22245 at 548.644 kb on - strand, within BPHYT_RS22245 at 548.644 kb on - strand, within BPHYT_RS22245 at 548.644 kb on - strand, within BPHYT_RS22245 at 548.700 kb on + strand, within BPHYT_RS22245 at 548.700 kb on + strand, within BPHYT_RS22245 at 548.700 kb on + strand, within BPHYT_RS22245 at 548.701 kb on - strand, within BPHYT_RS22245 at 548.781 kb on + strand, within BPHYT_RS22245 at 548.782 kb on - strand, within BPHYT_RS22245 at 548.892 kb on - strand, within BPHYT_RS22245 at 549.367 kb on - strand, within BPHYT_RS22245 at 549.501 kb on + strand at 549.501 kb on + strand at 549.502 kb on - strand at 549.502 kb on - strand at 549.857 kb on - strand, within BPHYT_RS22250 at 550.087 kb on - strand, within BPHYT_RS22250
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 547,112 - BPHYT_RS22235 0.61 +0.2 547,217 - BPHYT_RS22235 0.69 +1.1 547,217 - BPHYT_RS22235 0.69 -0.1 547,523 + -0.6 547,524 - -0.0 547,525 + -0.2 547,526 - -1.7 547,687 - -0.2 547,690 - +0.2 547,692 + +1.3 547,693 - +0.9 547,710 - -0.0 547,745 + +0.6 547,746 - +0.2 547,814 - -0.0 547,816 + +1.0 547,816 + +0.4 547,816 + +0.8 547,816 + +2.5 547,817 - -0.1 547,972 + BPHYT_RS22240 0.29 +0.8 547,975 - BPHYT_RS22240 0.30 +0.4 547,975 - BPHYT_RS22240 0.30 +0.4 548,042 - BPHYT_RS22240 0.64 -0.2 548,086 + BPHYT_RS22240 0.86 -0.1 548,087 - BPHYT_RS22240 0.87 +1.6 548,298 + BPHYT_RS22245 0.13 +0.4 548,307 + BPHYT_RS22245 0.13 +0.6 548,338 - BPHYT_RS22245 0.15 +0.5 548,538 + BPHYT_RS22245 0.29 +1.3 548,539 - BPHYT_RS22245 0.29 +2.0 548,644 - BPHYT_RS22245 0.36 +1.6 548,644 - BPHYT_RS22245 0.36 +1.0 548,644 - BPHYT_RS22245 0.36 +1.3 548,700 + BPHYT_RS22245 0.40 +0.1 548,700 + BPHYT_RS22245 0.40 +0.1 548,700 + BPHYT_RS22245 0.40 -0.0 548,701 - BPHYT_RS22245 0.40 +1.2 548,781 + BPHYT_RS22245 0.45 +0.5 548,782 - BPHYT_RS22245 0.45 +1.4 548,892 - BPHYT_RS22245 0.53 -0.6 549,367 - BPHYT_RS22245 0.85 +0.4 549,501 + +2.3 549,501 + +2.2 549,502 - +0.3 549,502 - +0.8 549,857 - BPHYT_RS22250 0.15 -0.3 550,087 - BPHYT_RS22250 0.34 -0.2
Or see this region's nucleotide sequence