Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22090 and BPHYT_RS22095 are separated by 224 nucleotides BPHYT_RS22095 and BPHYT_RS22100 are separated by 418 nucleotides
BPHYT_RS22090: BPHYT_RS22090 - hypothetical protein, at 513,266 to 514,138
_RS22090
BPHYT_RS22095: BPHYT_RS22095 - porin, at 514,363 to 515,544
_RS22095
BPHYT_RS22100: BPHYT_RS22100 - ribonuclease BN, at 515,963 to 517,294
_RS22100
Position (kb) 514
515
516 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 513.394 kb on + strand, within BPHYT_RS22090 at 513.395 kb on - strand, within BPHYT_RS22090 at 513.609 kb on + strand, within BPHYT_RS22090 at 513.654 kb on - strand, within BPHYT_RS22090 at 513.696 kb on - strand, within BPHYT_RS22090 at 513.855 kb on - strand, within BPHYT_RS22090 at 513.855 kb on - strand, within BPHYT_RS22090 at 513.878 kb on - strand, within BPHYT_RS22090 at 513.911 kb on + strand, within BPHYT_RS22090 at 513.911 kb on + strand, within BPHYT_RS22090 at 514.136 kb on + strand at 514.422 kb on - strand at 514.428 kb on - strand at 514.474 kb on - strand at 514.474 kb on - strand at 514.515 kb on - strand, within BPHYT_RS22095 at 514.541 kb on + strand, within BPHYT_RS22095 at 514.568 kb on + strand, within BPHYT_RS22095 at 514.602 kb on - strand, within BPHYT_RS22095 at 514.602 kb on - strand, within BPHYT_RS22095 at 514.607 kb on + strand, within BPHYT_RS22095 at 514.607 kb on + strand, within BPHYT_RS22095 at 514.607 kb on + strand, within BPHYT_RS22095 at 514.608 kb on - strand, within BPHYT_RS22095 at 514.608 kb on - strand, within BPHYT_RS22095 at 514.652 kb on + strand, within BPHYT_RS22095 at 514.670 kb on + strand, within BPHYT_RS22095 at 514.671 kb on - strand, within BPHYT_RS22095 at 514.713 kb on - strand, within BPHYT_RS22095 at 514.760 kb on + strand, within BPHYT_RS22095 at 514.806 kb on - strand, within BPHYT_RS22095 at 514.917 kb on - strand, within BPHYT_RS22095 at 514.949 kb on + strand, within BPHYT_RS22095 at 514.950 kb on - strand, within BPHYT_RS22095 at 515.012 kb on + strand, within BPHYT_RS22095 at 515.012 kb on + strand at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.054 kb on + strand, within BPHYT_RS22095 at 515.055 kb on - strand, within BPHYT_RS22095 at 515.200 kb on - strand, within BPHYT_RS22095 at 515.432 kb on + strand at 515.433 kb on - strand at 515.459 kb on + strand at 515.523 kb on + strand at 515.567 kb on - strand at 515.592 kb on + strand at 515.592 kb on + strand at 515.760 kb on + strand at 515.760 kb on + strand at 515.760 kb on + strand at 515.761 kb on - strand at 515.796 kb on + strand at 515.797 kb on - strand at 515.805 kb on + strand at 515.805 kb on + strand at 515.805 kb on + strand at 516.064 kb on - strand at 516.489 kb on + strand, within BPHYT_RS22100 at 516.489 kb on + strand, within BPHYT_RS22100
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 513,394 + BPHYT_RS22090 0.15 +0.8 513,395 - BPHYT_RS22090 0.15 +0.4 513,609 + BPHYT_RS22090 0.39 -1.0 513,654 - BPHYT_RS22090 0.44 -0.7 513,696 - BPHYT_RS22090 0.49 +0.8 513,855 - BPHYT_RS22090 0.67 +0.5 513,855 - BPHYT_RS22090 0.67 -0.3 513,878 - BPHYT_RS22090 0.70 -1.0 513,911 + BPHYT_RS22090 0.74 -0.6 513,911 + BPHYT_RS22090 0.74 -2.8 514,136 + +2.9 514,422 - -0.0 514,428 - +0.1 514,474 - -1.3 514,474 - +0.1 514,515 - BPHYT_RS22095 0.13 +0.5 514,541 + BPHYT_RS22095 0.15 -0.3 514,568 + BPHYT_RS22095 0.17 -0.7 514,602 - BPHYT_RS22095 0.20 -2.1 514,602 - BPHYT_RS22095 0.20 -0.6 514,607 + BPHYT_RS22095 0.21 +2.3 514,607 + BPHYT_RS22095 0.21 +0.2 514,607 + BPHYT_RS22095 0.21 -0.3 514,608 - BPHYT_RS22095 0.21 +0.1 514,608 - BPHYT_RS22095 0.21 -0.2 514,652 + BPHYT_RS22095 0.24 -0.6 514,670 + BPHYT_RS22095 0.26 -1.7 514,671 - BPHYT_RS22095 0.26 -0.6 514,713 - BPHYT_RS22095 0.30 -2.1 514,760 + BPHYT_RS22095 0.34 -2.0 514,806 - BPHYT_RS22095 0.37 -1.3 514,917 - BPHYT_RS22095 0.47 -0.2 514,949 + BPHYT_RS22095 0.50 +1.8 514,950 - BPHYT_RS22095 0.50 -0.5 515,012 + BPHYT_RS22095 0.55 -1.4 515,012 + +0.7 515,013 - BPHYT_RS22095 0.55 -2.6 515,013 - BPHYT_RS22095 0.55 -0.1 515,013 - BPHYT_RS22095 0.55 -1.4 515,054 + BPHYT_RS22095 0.58 -2.9 515,055 - BPHYT_RS22095 0.59 -1.2 515,200 - BPHYT_RS22095 0.71 -0.9 515,432 + -0.5 515,433 - -1.2 515,459 + -1.9 515,523 + +0.2 515,567 - +0.6 515,592 + -0.8 515,592 + -0.2 515,760 + -1.3 515,760 + -1.2 515,760 + +0.2 515,761 - -0.7 515,796 + -1.0 515,797 - -0.3 515,805 + -1.0 515,805 + -0.2 515,805 + -0.3 516,064 - -1.0 516,489 + BPHYT_RS22100 0.39 +0.1 516,489 + BPHYT_RS22100 0.39 +0.5
Or see this region's nucleotide sequence