Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21740 and BPHYT_RS21745 are separated by 205 nucleotides BPHYT_RS21745 and BPHYT_RS21750 are separated by 189 nucleotides
BPHYT_RS21740: BPHYT_RS21740 - IclR family transcriptional regulator, at 433,809 to 434,645
_RS21740
BPHYT_RS21745: BPHYT_RS21745 - choline dehydrogenase, at 434,851 to 436,506
_RS21745
BPHYT_RS21750: BPHYT_RS21750 - major facilitator transporter, at 436,696 to 438,006
_RS21750
Position (kb)
434
435
436
437 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 434.097 kb on - strand, within BPHYT_RS21740 at 434.097 kb on - strand, within BPHYT_RS21740 at 434.263 kb on + strand, within BPHYT_RS21740 at 434.263 kb on + strand, within BPHYT_RS21740 at 434.456 kb on - strand at 434.715 kb on + strand at 435.043 kb on + strand, within BPHYT_RS21745 at 435.044 kb on - strand, within BPHYT_RS21745 at 435.136 kb on + strand, within BPHYT_RS21745 at 435.137 kb on - strand, within BPHYT_RS21745 at 435.137 kb on - strand, within BPHYT_RS21745 at 435.404 kb on - strand, within BPHYT_RS21745 at 435.452 kb on - strand, within BPHYT_RS21745 at 435.751 kb on + strand, within BPHYT_RS21745 at 435.752 kb on - strand, within BPHYT_RS21745 at 435.992 kb on - strand, within BPHYT_RS21745 at 436.082 kb on - strand, within BPHYT_RS21745 at 436.269 kb on + strand, within BPHYT_RS21745 at 436.392 kb on + strand at 436.583 kb on - strand at 436.583 kb on - strand at 436.608 kb on - strand at 436.659 kb on + strand at 436.696 kb on - strand at 437.116 kb on + strand, within BPHYT_RS21750 at 437.299 kb on + strand, within BPHYT_RS21750 at 437.432 kb on - strand, within BPHYT_RS21750
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 434,097 - BPHYT_RS21740 0.34 +0.3 434,097 - BPHYT_RS21740 0.34 -0.4 434,263 + BPHYT_RS21740 0.54 -0.6 434,263 + BPHYT_RS21740 0.54 -0.5 434,456 - +1.1 434,715 + +1.0 435,043 + BPHYT_RS21745 0.12 +0.4 435,044 - BPHYT_RS21745 0.12 +0.6 435,136 + BPHYT_RS21745 0.17 -0.2 435,137 - BPHYT_RS21745 0.17 +0.5 435,137 - BPHYT_RS21745 0.17 +0.8 435,404 - BPHYT_RS21745 0.33 +0.1 435,452 - BPHYT_RS21745 0.36 -0.5 435,751 + BPHYT_RS21745 0.54 +1.7 435,752 - BPHYT_RS21745 0.54 -0.9 435,992 - BPHYT_RS21745 0.69 +4.1 436,082 - BPHYT_RS21745 0.74 +1.1 436,269 + BPHYT_RS21745 0.86 -0.2 436,392 + -0.3 436,583 - -0.1 436,583 - -1.2 436,608 - -0.3 436,659 + -0.5 436,696 - -0.2 437,116 + BPHYT_RS21750 0.32 -0.7 437,299 + BPHYT_RS21750 0.46 +0.1 437,432 - BPHYT_RS21750 0.56 -1.2
Or see this region's nucleotide sequence