Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21205 and BPHYT_RS21210 are separated by 116 nucleotides BPHYT_RS21210 and BPHYT_RS21215 are separated by 130 nucleotides
BPHYT_RS21205: BPHYT_RS21205 - high-affinity nickel-transporter, at 319,331 to 320,455
_RS21205
BPHYT_RS21210: BPHYT_RS21210 - hypothetical protein, at 320,572 to 321,891
_RS21210
BPHYT_RS21215: BPHYT_RS21215 - Crp/Fnr family transcriptional regulator, at 322,022 to 322,678
_RS21215
Position (kb)
320
321
322 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 319.766 kb on - strand, within BPHYT_RS21205 at 319.899 kb on + strand, within BPHYT_RS21205 at 319.900 kb on - strand, within BPHYT_RS21205 at 320.050 kb on - strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.451 kb on + strand at 320.606 kb on + strand at 320.618 kb on + strand at 320.618 kb on + strand at 320.696 kb on + strand at 320.696 kb on + strand at 320.697 kb on - strand at 320.697 kb on - strand at 320.735 kb on + strand, within BPHYT_RS21210 at 320.996 kb on + strand, within BPHYT_RS21210 at 321.208 kb on - strand, within BPHYT_RS21210 at 321.407 kb on - strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.423 kb on - strand, within BPHYT_RS21210 at 321.749 kb on + strand, within BPHYT_RS21210 at 322.023 kb on + strand at 322.024 kb on - strand at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.545 kb on + strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.575 kb on + strand, within BPHYT_RS21215 at 322.813 kb on + strand at 322.813 kb on + strand at 322.813 kb on + strand at 322.845 kb on + strand at 322.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 319,766 - BPHYT_RS21205 0.39 -1.5 319,899 + BPHYT_RS21205 0.50 -0.8 319,900 - BPHYT_RS21205 0.51 +0.6 320,050 - BPHYT_RS21205 0.64 -2.5 320,211 + BPHYT_RS21205 0.78 -0.6 320,211 + BPHYT_RS21205 0.78 +0.1 320,451 + +0.7 320,606 + +0.6 320,618 + +0.2 320,618 + +0.2 320,696 + -1.6 320,696 + +0.1 320,697 - -0.1 320,697 - -0.7 320,735 + BPHYT_RS21210 0.12 -0.2 320,996 + BPHYT_RS21210 0.32 +0.2 321,208 - BPHYT_RS21210 0.48 +1.1 321,407 - BPHYT_RS21210 0.63 -0.3 321,422 + BPHYT_RS21210 0.64 +2.1 321,422 + BPHYT_RS21210 0.64 -0.4 321,423 - BPHYT_RS21210 0.64 -0.5 321,749 + BPHYT_RS21210 0.89 +0.0 322,023 + +0.3 322,024 - -1.4 322,324 + BPHYT_RS21215 0.46 -0.5 322,324 + BPHYT_RS21215 0.46 -0.1 322,324 + BPHYT_RS21215 0.46 +1.3 322,324 + BPHYT_RS21215 0.46 +1.7 322,325 - BPHYT_RS21215 0.46 +1.6 322,325 - BPHYT_RS21215 0.46 +1.7 322,545 + BPHYT_RS21215 0.80 +2.7 322,546 - BPHYT_RS21215 0.80 +1.7 322,546 - BPHYT_RS21215 0.80 +3.3 322,575 + BPHYT_RS21215 0.84 -0.3 322,813 + -0.3 322,813 + +0.7 322,813 + +0.1 322,845 + +1.8 322,846 - +0.2
Or see this region's nucleotide sequence