Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21130 and BPHYT_RS21135 overlap by 4 nucleotides BPHYT_RS21135 and BPHYT_RS21140 are separated by 32 nucleotides BPHYT_RS21140 and BPHYT_RS21145 are separated by 143 nucleotides
BPHYT_RS21130: BPHYT_RS21130 - F420-dependent oxidoreductase, at 301,771 to 302,550
_RS21130
BPHYT_RS21135: BPHYT_RS21135 - NADH-ubiquinone oxidoreductase subunit 6, at 302,547 to 303,227
_RS21135
BPHYT_RS21140: BPHYT_RS21140 - methylenetetrahydromethanopterin reductase, at 303,260 to 304,261
_RS21140
BPHYT_RS21145: BPHYT_RS21145 - allantoate amidohydrolase, at 304,405 to 305,643
_RS21145
Position (kb) 303
304
305 Strain fitness (log2 ratio) -2
-1
0
1 at 302.621 kb on - strand, within BPHYT_RS21135 at 302.692 kb on + strand, within BPHYT_RS21135 at 302.693 kb on - strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135 at 302.944 kb on + strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 303.225 kb on + strand at 303.225 kb on + strand at 303.226 kb on - strand at 303.975 kb on - strand, within BPHYT_RS21140 at 304.038 kb on - strand, within BPHYT_RS21140 at 304.190 kb on + strand at 304.773 kb on + strand, within BPHYT_RS21145
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 302,621 - BPHYT_RS21135 0.11 +0.1 302,692 + BPHYT_RS21135 0.21 +1.3 302,693 - BPHYT_RS21135 0.21 -0.9 302,698 + BPHYT_RS21135 0.22 +1.6 302,698 + BPHYT_RS21135 0.22 -1.6 302,944 + BPHYT_RS21135 0.58 +1.3 302,945 - BPHYT_RS21135 0.58 -0.6 302,945 - BPHYT_RS21135 0.58 +0.5 302,945 - BPHYT_RS21135 0.58 -0.6 303,225 + +0.1 303,225 + -1.8 303,226 - +0.7 303,975 - BPHYT_RS21140 0.71 +0.4 304,038 - BPHYT_RS21140 0.78 +0.2 304,190 + +1.5 304,773 + BPHYT_RS21145 0.30 -1.0
Or see this region's nucleotide sequence