Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21120 and BPHYT_RS21125 are separated by 173 nucleotides BPHYT_RS21125 and BPHYT_RS21130 overlap by 4 nucleotides BPHYT_RS21130 and BPHYT_RS21135 overlap by 4 nucleotides BPHYT_RS21135 and BPHYT_RS21140 are separated by 32 nucleotides
BPHYT_RS21120: BPHYT_RS21120 - hypothetical protein, at 300,132 to 300,959
_RS21120
BPHYT_RS21125: BPHYT_RS21125 - hypothetical protein, at 301,133 to 301,774
_RS21125
BPHYT_RS21130: BPHYT_RS21130 - F420-dependent oxidoreductase, at 301,771 to 302,550
_RS21130
BPHYT_RS21135: BPHYT_RS21135 - NADH-ubiquinone oxidoreductase subunit 6, at 302,547 to 303,227
_RS21135
BPHYT_RS21140: BPHYT_RS21140 - methylenetetrahydromethanopterin reductase, at 303,260 to 304,261
_RS21140
Position (kb) 301
302
303 Strain fitness (log2 ratio) -2
-1
0
1 at 300.918 kb on + strand at 301.132 kb on + strand at 301.809 kb on - strand at 301.969 kb on + strand, within BPHYT_RS21130 at 301.969 kb on + strand, within BPHYT_RS21130 at 301.969 kb on + strand, within BPHYT_RS21130 at 302.621 kb on - strand, within BPHYT_RS21135 at 302.692 kb on + strand, within BPHYT_RS21135 at 302.693 kb on - strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135 at 302.944 kb on + strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 303.225 kb on + strand at 303.225 kb on + strand at 303.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 300,918 + +0.5 301,132 + -1.3 301,809 - +1.1 301,969 + BPHYT_RS21130 0.25 -0.6 301,969 + BPHYT_RS21130 0.25 -1.3 301,969 + BPHYT_RS21130 0.25 -0.7 302,621 - BPHYT_RS21135 0.11 +0.1 302,692 + BPHYT_RS21135 0.21 +1.3 302,693 - BPHYT_RS21135 0.21 -0.9 302,698 + BPHYT_RS21135 0.22 +1.6 302,698 + BPHYT_RS21135 0.22 -1.6 302,944 + BPHYT_RS21135 0.58 +1.3 302,945 - BPHYT_RS21135 0.58 -0.6 302,945 - BPHYT_RS21135 0.58 +0.5 302,945 - BPHYT_RS21135 0.58 -0.6 303,225 + +0.1 303,225 + -1.8 303,226 - +0.7
Or see this region's nucleotide sequence