Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21115 and BPHYT_RS21120 overlap by 8 nucleotides BPHYT_RS21120 and BPHYT_RS21125 are separated by 173 nucleotides BPHYT_RS21125 and BPHYT_RS21130 overlap by 4 nucleotides
BPHYT_RS21115: BPHYT_RS21115 - radical SAM protein, at 298,868 to 300,139
_RS21115
BPHYT_RS21120: BPHYT_RS21120 - hypothetical protein, at 300,132 to 300,959
_RS21120
BPHYT_RS21125: BPHYT_RS21125 - hypothetical protein, at 301,133 to 301,774
_RS21125
BPHYT_RS21130: BPHYT_RS21130 - F420-dependent oxidoreductase, at 301,771 to 302,550
_RS21130
Position (kb) 300
301 Strain fitness (log2 ratio) -2
-1
0
1 at 299.356 kb on - strand, within BPHYT_RS21115 at 299.361 kb on - strand, within BPHYT_RS21115 at 299.410 kb on + strand, within BPHYT_RS21115 at 299.555 kb on - strand, within BPHYT_RS21115 at 299.571 kb on - strand, within BPHYT_RS21115 at 299.696 kb on + strand, within BPHYT_RS21115 at 299.697 kb on - strand, within BPHYT_RS21115 at 299.749 kb on + strand, within BPHYT_RS21115 at 299.750 kb on - strand, within BPHYT_RS21115 at 299.823 kb on + strand, within BPHYT_RS21115 at 300.002 kb on + strand, within BPHYT_RS21115 at 300.068 kb on + strand at 300.313 kb on - strand, within BPHYT_RS21120 at 300.434 kb on + strand, within BPHYT_RS21120 at 300.434 kb on + strand, within BPHYT_RS21120 at 300.435 kb on - strand, within BPHYT_RS21120 at 300.507 kb on - strand, within BPHYT_RS21120 at 300.535 kb on + strand, within BPHYT_RS21120 at 300.535 kb on + strand, within BPHYT_RS21120 at 300.536 kb on - strand, within BPHYT_RS21120 at 300.918 kb on + strand at 301.132 kb on + strand at 301.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 299,356 - BPHYT_RS21115 0.38 +0.2 299,361 - BPHYT_RS21115 0.39 -0.7 299,410 + BPHYT_RS21115 0.43 -0.9 299,555 - BPHYT_RS21115 0.54 -0.1 299,571 - BPHYT_RS21115 0.55 +0.3 299,696 + BPHYT_RS21115 0.65 -0.5 299,697 - BPHYT_RS21115 0.65 +0.4 299,749 + BPHYT_RS21115 0.69 +0.5 299,750 - BPHYT_RS21115 0.69 -0.4 299,823 + BPHYT_RS21115 0.75 +0.6 300,002 + BPHYT_RS21115 0.89 +0.8 300,068 + -1.2 300,313 - BPHYT_RS21120 0.22 -2.0 300,434 + BPHYT_RS21120 0.36 -1.3 300,434 + BPHYT_RS21120 0.36 +0.5 300,435 - BPHYT_RS21120 0.37 -1.1 300,507 - BPHYT_RS21120 0.45 +0.6 300,535 + BPHYT_RS21120 0.49 -1.0 300,535 + BPHYT_RS21120 0.49 -0.7 300,536 - BPHYT_RS21120 0.49 -0.1 300,918 + +0.5 301,132 + -1.3 301,809 - +1.1
Or see this region's nucleotide sequence