Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20675 and BPHYT_RS20680 are separated by 191 nucleotides BPHYT_RS20680 and BPHYT_RS20685 are separated by 72 nucleotides
BPHYT_RS20675: BPHYT_RS20675 - LysR family transcriptional regulator, at 198,610 to 199,482
_RS20675
BPHYT_RS20680: BPHYT_RS20680 - LacI family transcriptional regulator, at 199,674 to 200,639
_RS20680
BPHYT_RS20685: BPHYT_RS20685 - ribose ABC transporter ATPase, at 200,712 to 202,325
_RS20685
Position (kb) 199
200
201 Strain fitness (log2 ratio) -1
0
1 at 198.962 kb on + strand, within BPHYT_RS20675 at 198.962 kb on + strand, within BPHYT_RS20675 at 199.016 kb on + strand, within BPHYT_RS20675 at 199.016 kb on + strand, within BPHYT_RS20675 at 199.082 kb on + strand, within BPHYT_RS20675 at 199.163 kb on + strand, within BPHYT_RS20675 at 199.241 kb on - strand, within BPHYT_RS20675 at 199.537 kb on - strand at 200.195 kb on + strand, within BPHYT_RS20680 at 200.521 kb on - strand, within BPHYT_RS20680 at 200.521 kb on - strand, within BPHYT_RS20680 at 200.781 kb on + strand at 201.330 kb on + strand, within BPHYT_RS20685 at 201.331 kb on - strand, within BPHYT_RS20685 at 201.457 kb on + strand, within BPHYT_RS20685 at 201.501 kb on - strand, within BPHYT_RS20685
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 198,962 + BPHYT_RS20675 0.40 +0.8 198,962 + BPHYT_RS20675 0.40 +0.4 199,016 + BPHYT_RS20675 0.47 +0.9 199,016 + BPHYT_RS20675 0.47 -0.7 199,082 + BPHYT_RS20675 0.54 +0.1 199,163 + BPHYT_RS20675 0.63 +0.7 199,241 - BPHYT_RS20675 0.72 -0.4 199,537 - +0.7 200,195 + BPHYT_RS20680 0.54 -0.2 200,521 - BPHYT_RS20680 0.88 -0.4 200,521 - BPHYT_RS20680 0.88 -0.2 200,781 + -1.1 201,330 + BPHYT_RS20685 0.38 +0.6 201,331 - BPHYT_RS20685 0.38 +0.4 201,457 + BPHYT_RS20685 0.46 +1.2 201,501 - BPHYT_RS20685 0.49 -0.2
Or see this region's nucleotide sequence