Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20555 and BPHYT_RS20560 are separated by 118 nucleotides BPHYT_RS20560 and BPHYT_RS20565 are separated by 70 nucleotides BPHYT_RS20565 and BPHYT_RS20570 are separated by 261 nucleotides
BPHYT_RS20555: BPHYT_RS20555 - serine/threonine protein kinase, at 173,034 to 174,371
_RS20555
BPHYT_RS20560: BPHYT_RS20560 - sugar ABC transporter substrate-binding protein, at 174,490 to 175,440
_RS20560
BPHYT_RS20565: BPHYT_RS20565 - photosystem reaction center subunit H, at 175,511 to 175,942
_RS20565
BPHYT_RS20570: BPHYT_RS20570 - heptosyltransferase, at 176,204 to 177,379
_RS20570
Position (kb)
174
175
176 Strain fitness (log2 ratio)
-2
-1
0
1 at 173.575 kb on - strand, within BPHYT_RS20555 at 173.701 kb on - strand, within BPHYT_RS20555 at 173.960 kb on + strand, within BPHYT_RS20555 at 173.964 kb on + strand, within BPHYT_RS20555 at 173.964 kb on + strand, within BPHYT_RS20555 at 173.965 kb on - strand, within BPHYT_RS20555 at 174.072 kb on + strand, within BPHYT_RS20555 at 174.083 kb on + strand, within BPHYT_RS20555 at 174.141 kb on + strand, within BPHYT_RS20555 at 174.141 kb on + strand, within BPHYT_RS20555 at 174.142 kb on - strand, within BPHYT_RS20555 at 174.412 kb on + strand at 174.415 kb on + strand at 174.415 kb on + strand at 174.416 kb on - strand at 174.562 kb on - strand at 174.649 kb on + strand, within BPHYT_RS20560 at 174.661 kb on + strand, within BPHYT_RS20560 at 174.946 kb on + strand, within BPHYT_RS20560 at 174.946 kb on + strand, within BPHYT_RS20560 at 174.947 kb on - strand, within BPHYT_RS20560 at 175.099 kb on + strand, within BPHYT_RS20560 at 175.234 kb on + strand, within BPHYT_RS20560 at 175.246 kb on + strand, within BPHYT_RS20560 at 175.561 kb on - strand, within BPHYT_RS20565 at 175.659 kb on + strand, within BPHYT_RS20565 at 175.659 kb on + strand, within BPHYT_RS20565 at 175.660 kb on - strand, within BPHYT_RS20565 at 175.704 kb on + strand, within BPHYT_RS20565 at 175.965 kb on + strand at 175.966 kb on - strand at 176.048 kb on - strand at 176.258 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 173,575 - BPHYT_RS20555 0.40 -0.1 173,701 - BPHYT_RS20555 0.50 -0.3 173,960 + BPHYT_RS20555 0.69 +0.4 173,964 + BPHYT_RS20555 0.70 -2.1 173,964 + BPHYT_RS20555 0.70 -0.3 173,965 - BPHYT_RS20555 0.70 -0.5 174,072 + BPHYT_RS20555 0.78 -0.2 174,083 + BPHYT_RS20555 0.78 +1.6 174,141 + BPHYT_RS20555 0.83 -0.6 174,141 + BPHYT_RS20555 0.83 -0.1 174,142 - BPHYT_RS20555 0.83 -0.1 174,412 + -0.5 174,415 + -0.9 174,415 + -0.7 174,416 - +0.4 174,562 - -0.6 174,649 + BPHYT_RS20560 0.17 +1.1 174,661 + BPHYT_RS20560 0.18 -0.4 174,946 + BPHYT_RS20560 0.48 -0.4 174,946 + BPHYT_RS20560 0.48 -1.4 174,947 - BPHYT_RS20560 0.48 +0.9 175,099 + BPHYT_RS20560 0.64 -1.5 175,234 + BPHYT_RS20560 0.78 +1.3 175,246 + BPHYT_RS20560 0.79 -0.4 175,561 - BPHYT_RS20565 0.12 -0.2 175,659 + BPHYT_RS20565 0.34 +0.6 175,659 + BPHYT_RS20565 0.34 +0.2 175,660 - BPHYT_RS20565 0.34 -0.5 175,704 + BPHYT_RS20565 0.45 -0.9 175,965 + -1.3 175,966 - -0.5 176,048 - +0.2 176,258 + +0.3
Or see this region's nucleotide sequence