Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20485

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20480 and BPHYT_RS20485 are separated by 200 nucleotidesBPHYT_RS20485 and BPHYT_RS20490 are separated by 327 nucleotides BPHYT_RS20480: BPHYT_RS20480 - hypothetical protein, at 156,492 to 157,259 _RS20480 BPHYT_RS20485: BPHYT_RS20485 - chemotaxis protein, at 157,460 to 159,007 _RS20485 BPHYT_RS20490: BPHYT_RS20490 - porin, at 159,335 to 160,393 _RS20490 Position (kb) 157 158 159 160Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7 8at 156.711 kb on + strand, within BPHYT_RS20480at 156.712 kb on - strand, within BPHYT_RS20480at 156.861 kb on + strand, within BPHYT_RS20480at 156.861 kb on + strand, within BPHYT_RS20480at 156.862 kb on - strand, within BPHYT_RS20480at 156.862 kb on - strand, within BPHYT_RS20480at 156.975 kb on + strand, within BPHYT_RS20480at 156.975 kb on + strand, within BPHYT_RS20480at 156.982 kb on - strand, within BPHYT_RS20480at 156.982 kb on - strand, within BPHYT_RS20480at 156.982 kb on - strand, within BPHYT_RS20480at 156.982 kb on - strand, within BPHYT_RS20480at 157.071 kb on + strand, within BPHYT_RS20480at 157.072 kb on - strand, within BPHYT_RS20480at 157.134 kb on + strand, within BPHYT_RS20480at 157.660 kb on + strand, within BPHYT_RS20485at 157.660 kb on + strand, within BPHYT_RS20485at 157.707 kb on + strand, within BPHYT_RS20485at 157.728 kb on - strand, within BPHYT_RS20485at 157.835 kb on + strand, within BPHYT_RS20485at 157.835 kb on + strand, within BPHYT_RS20485at 157.835 kb on + strand, within BPHYT_RS20485at 157.835 kb on + strand, within BPHYT_RS20485at 157.952 kb on + strand, within BPHYT_RS20485at 158.130 kb on - strand, within BPHYT_RS20485at 158.225 kb on + strand, within BPHYT_RS20485at 158.225 kb on + strand, within BPHYT_RS20485at 158.226 kb on - strand, within BPHYT_RS20485at 158.584 kb on + strand, within BPHYT_RS20485at 158.584 kb on + strand, within BPHYT_RS20485at 158.584 kb on + strand, within BPHYT_RS20485at 159.102 kb on + strandat 159.102 kb on + strandat 159.173 kb on + strandat 159.173 kb on + strandat 159.267 kb on - strandat 159.459 kb on - strand, within BPHYT_RS20490at 159.486 kb on + strand, within BPHYT_RS20490at 159.655 kb on - strand, within BPHYT_RS20490at 159.719 kb on + strand, within BPHYT_RS20490at 159.720 kb on - strand, within BPHYT_RS20490at 159.731 kb on + strand, within BPHYT_RS20490at 159.731 kb on + strand, within BPHYT_RS20490at 159.731 kb on + strandat 159.732 kb on - strand, within BPHYT_RS20490at 159.732 kb on - strand, within BPHYT_RS20490at 159.732 kb on - strand, within BPHYT_RS20490at 159.732 kb on - strand, within BPHYT_RS20490at 159.836 kb on + strand, within BPHYT_RS20490at 159.837 kb on - strand, within BPHYT_RS20490at 159.927 kb on - strand, within BPHYT_RS20490at 159.929 kb on + strand, within BPHYT_RS20490at 159.930 kb on - strand, within BPHYT_RS20490at 159.934 kb on - strand, within BPHYT_RS20490at 159.972 kb on - strand, within BPHYT_RS20490

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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156,711 + BPHYT_RS20480 0.29 -0.3
156,712 - BPHYT_RS20480 0.29 -0.2
156,861 + BPHYT_RS20480 0.48 +0.2
156,861 + BPHYT_RS20480 0.48 +0.6
156,862 - BPHYT_RS20480 0.48 -0.4
156,862 - BPHYT_RS20480 0.48 -0.5
156,975 + BPHYT_RS20480 0.63 -0.2
156,975 + BPHYT_RS20480 0.63 -0.4
156,982 - BPHYT_RS20480 0.64 -0.5
156,982 - BPHYT_RS20480 0.64 +0.3
156,982 - BPHYT_RS20480 0.64 +1.3
156,982 - BPHYT_RS20480 0.64 +8.6
157,071 + BPHYT_RS20480 0.75 -1.3
157,072 - BPHYT_RS20480 0.76 +0.9
157,134 + BPHYT_RS20480 0.84 +1.1
157,660 + BPHYT_RS20485 0.13 +0.9
157,660 + BPHYT_RS20485 0.13 -0.3
157,707 + BPHYT_RS20485 0.16 +2.2
157,728 - BPHYT_RS20485 0.17 +0.7
157,835 + BPHYT_RS20485 0.24 -1.1
157,835 + BPHYT_RS20485 0.24 +0.5
157,835 + BPHYT_RS20485 0.24 +0.5
157,835 + BPHYT_RS20485 0.24 +0.3
157,952 + BPHYT_RS20485 0.32 -0.8
158,130 - BPHYT_RS20485 0.43 +1.1
158,225 + BPHYT_RS20485 0.49 -0.8
158,225 + BPHYT_RS20485 0.49 -0.5
158,226 - BPHYT_RS20485 0.49 -2.2
158,584 + BPHYT_RS20485 0.73 +0.2
158,584 + BPHYT_RS20485 0.73 +0.1
158,584 + BPHYT_RS20485 0.73 -2.1
159,102 + -0.0
159,102 + -2.6
159,173 + -0.5
159,173 + -1.2
159,267 - -0.8
159,459 - BPHYT_RS20490 0.12 +4.3
159,486 + BPHYT_RS20490 0.14 -1.6
159,655 - BPHYT_RS20490 0.30 -0.2
159,719 + BPHYT_RS20490 0.36 +0.5
159,720 - BPHYT_RS20490 0.36 -0.1
159,731 + BPHYT_RS20490 0.37 +0.5
159,731 + BPHYT_RS20490 0.37 +1.2
159,731 + -0.5
159,732 - BPHYT_RS20490 0.37 -0.7
159,732 - BPHYT_RS20490 0.37 -0.5
159,732 - BPHYT_RS20490 0.37 -0.0
159,732 - BPHYT_RS20490 0.37 +1.6
159,836 + BPHYT_RS20490 0.47 +1.8
159,837 - BPHYT_RS20490 0.47 +0.5
159,927 - BPHYT_RS20490 0.56 +0.2
159,929 + BPHYT_RS20490 0.56 -1.3
159,930 - BPHYT_RS20490 0.56 +0.3
159,934 - BPHYT_RS20490 0.57 +0.4
159,972 - BPHYT_RS20490 0.60 +0.9

Or see this region's nucleotide sequence