Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20130 and BPHYT_RS20135 are separated by 196 nucleotides BPHYT_RS20135 and BPHYT_RS20140 are separated by 37 nucleotides BPHYT_RS20140 and BPHYT_RS20145 overlap by 4 nucleotides BPHYT_RS20145 and BPHYT_RS20150 are separated by 77 nucleotides
BPHYT_RS20130: BPHYT_RS20130 - diguanylate phosphodiesterase, at 59,338 to 60,105
_RS20130
BPHYT_RS20135: BPHYT_RS20135 - UDP-N-acetyl-D-mannosamine transferase, at 60,302 to 61,060
_RS20135
BPHYT_RS20140: BPHYT_RS20140 - hypothetical protein, at 61,098 to 62,264
_RS20140
BPHYT_RS20145: BPHYT_RS20145 - hypothetical protein, at 62,261 to 62,977
_RS20145
BPHYT_RS20150: BPHYT_RS20150 - glycosyl transferase family 1, at 63,055 to 64,146
_RS20150
Position (kb) 61
62
63 Strain fitness (log2 ratio) -2
-1
0
1 at 60.135 kb on - strand at 60.135 kb on - strand at 60.401 kb on - strand, within BPHYT_RS20135 at 60.628 kb on + strand, within BPHYT_RS20135 at 60.855 kb on - strand, within BPHYT_RS20135 at 60.875 kb on + strand, within BPHYT_RS20135 at 60.875 kb on + strand, within BPHYT_RS20135 at 61.139 kb on - strand at 61.232 kb on - strand, within BPHYT_RS20140 at 61.311 kb on - strand, within BPHYT_RS20140 at 61.408 kb on + strand, within BPHYT_RS20140 at 61.437 kb on - strand, within BPHYT_RS20140 at 61.699 kb on + strand, within BPHYT_RS20140 at 61.700 kb on - strand, within BPHYT_RS20140 at 61.769 kb on + strand, within BPHYT_RS20140 at 62.160 kb on + strand at 62.161 kb on - strand at 62.592 kb on + strand, within BPHYT_RS20145 at 63.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 60,135 - +1.1 60,135 - +1.7 60,401 - BPHYT_RS20135 0.13 +1.3 60,628 + BPHYT_RS20135 0.43 +0.1 60,855 - BPHYT_RS20135 0.73 +1.0 60,875 + BPHYT_RS20135 0.75 +0.2 60,875 + BPHYT_RS20135 0.75 -0.8 61,139 - -0.6 61,232 - BPHYT_RS20140 0.11 -0.3 61,311 - BPHYT_RS20140 0.18 -1.1 61,408 + BPHYT_RS20140 0.27 +0.6 61,437 - BPHYT_RS20140 0.29 -0.7 61,699 + BPHYT_RS20140 0.51 +1.3 61,700 - BPHYT_RS20140 0.52 -1.0 61,769 + BPHYT_RS20140 0.57 -1.6 62,160 + -0.9 62,161 - -1.5 62,592 + BPHYT_RS20145 0.46 -2.2 63,093 - +0.5
Or see this region's nucleotide sequence