Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18970 and BPHYT_RS18975 are separated by 5 nucleotides BPHYT_RS18975 and BPHYT_RS18980 overlap by 4 nucleotides BPHYT_RS18980 and BPHYT_RS18985 overlap by 4 nucleotides BPHYT_RS18985 and BPHYT_RS18990 are separated by 16 nucleotides
BPHYT_RS18970: BPHYT_RS18970 - chemotaxis protein, at 4,260,362 to 4,261,450
_RS18970
BPHYT_RS18975: BPHYT_RS18975 - chemotaxis protein CheD, at 4,261,456 to 4,262,271
_RS18975
BPHYT_RS18980: BPHYT_RS18980 - chemotaxis protein CheR, at 4,262,268 to 4,263,224
_RS18980
BPHYT_RS18985: BPHYT_RS18985 - hypothetical protein, at 4,263,221 to 4,263,412
_RS18985
BPHYT_RS18990: BPHYT_RS18990 - chemotaxis protein, at 4,263,429 to 4,265,282
_RS18990
Position (kb) 4262
4263
4264 Strain fitness (log2 ratio) -2
-1
0
1 at 4261.903 kb on + strand, within BPHYT_RS18975 at 4261.903 kb on + strand, within BPHYT_RS18975 at 4261.904 kb on - strand, within BPHYT_RS18975 at 4262.018 kb on + strand, within BPHYT_RS18975 at 4262.183 kb on + strand, within BPHYT_RS18975 at 4262.237 kb on + strand at 4262.237 kb on + strand at 4262.238 kb on - strand at 4262.238 kb on - strand at 4262.271 kb on + strand at 4262.271 kb on + strand at 4262.272 kb on - strand at 4262.365 kb on + strand, within BPHYT_RS18980 at 4262.366 kb on - strand, within BPHYT_RS18980 at 4262.404 kb on + strand, within BPHYT_RS18980 at 4262.405 kb on - strand, within BPHYT_RS18980 at 4262.504 kb on - strand, within BPHYT_RS18980 at 4262.704 kb on + strand, within BPHYT_RS18980 at 4262.705 kb on - strand, within BPHYT_RS18980 at 4262.705 kb on - strand, within BPHYT_RS18980 at 4263.058 kb on + strand, within BPHYT_RS18980 at 4263.059 kb on - strand, within BPHYT_RS18980 at 4263.243 kb on + strand, within BPHYT_RS18985 at 4263.243 kb on + strand, within BPHYT_RS18985 at 4263.283 kb on + strand, within BPHYT_RS18985 at 4263.284 kb on - strand, within BPHYT_RS18985 at 4263.430 kb on + strand at 4263.431 kb on - strand at 4263.590 kb on + strand at 4263.717 kb on - strand, within BPHYT_RS18990 at 4264.157 kb on + strand, within BPHYT_RS18990 at 4264.157 kb on + strand, within BPHYT_RS18990
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 4,261,903 + BPHYT_RS18975 0.55 -0.5 4,261,903 + BPHYT_RS18975 0.55 +0.5 4,261,904 - BPHYT_RS18975 0.55 -1.2 4,262,018 + BPHYT_RS18975 0.69 -0.4 4,262,183 + BPHYT_RS18975 0.89 -1.2 4,262,237 + -1.4 4,262,237 + -0.1 4,262,238 - +0.2 4,262,238 - -0.4 4,262,271 + -0.1 4,262,271 + -1.1 4,262,272 - -1.5 4,262,365 + BPHYT_RS18980 0.10 +0.6 4,262,366 - BPHYT_RS18980 0.10 +0.2 4,262,404 + BPHYT_RS18980 0.14 +0.1 4,262,405 - BPHYT_RS18980 0.14 -0.6 4,262,504 - BPHYT_RS18980 0.25 -0.8 4,262,704 + BPHYT_RS18980 0.46 -0.0 4,262,705 - BPHYT_RS18980 0.46 +0.7 4,262,705 - BPHYT_RS18980 0.46 +0.1 4,263,058 + BPHYT_RS18980 0.83 -0.2 4,263,059 - BPHYT_RS18980 0.83 +0.6 4,263,243 + BPHYT_RS18985 0.11 -1.1 4,263,243 + BPHYT_RS18985 0.11 -1.0 4,263,283 + BPHYT_RS18985 0.32 +1.1 4,263,284 - BPHYT_RS18985 0.33 +0.7 4,263,430 + -0.1 4,263,431 - -1.8 4,263,590 + -2.4 4,263,717 - BPHYT_RS18990 0.16 -0.7 4,264,157 + BPHYT_RS18990 0.39 -1.0 4,264,157 + BPHYT_RS18990 0.39 +0.1
Or see this region's nucleotide sequence