Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18950 and BPHYT_RS18955 are separated by 15 nucleotides BPHYT_RS18955 and BPHYT_RS18960 are separated by 288 nucleotides BPHYT_RS18960 and BPHYT_RS18965 are separated by 1 nucleotides BPHYT_RS18965 and BPHYT_RS18970 are separated by 86 nucleotides
BPHYT_RS18950: BPHYT_RS18950 - hypothetical protein, at 4,257,029 to 4,258,318
_RS18950
BPHYT_RS18955: BPHYT_RS18955 - hypothetical protein, at 4,258,334 to 4,258,879
_RS18955
BPHYT_RS18960: BPHYT_RS18960 - chemotaxis protein CheZ, at 4,259,168 to 4,259,878
_RS18960
BPHYT_RS18965: BPHYT_RS18965 - chemotaxis protein CheY, at 4,259,880 to 4,260,275
_RS18965
BPHYT_RS18970: BPHYT_RS18970 - chemotaxis protein, at 4,260,362 to 4,261,450
_RS18970
Position (kb)
4259
4260 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4258.303 kb on + strand at 4258.303 kb on + strand at 4258.303 kb on + strand at 4258.303 kb on + strand at 4258.303 kb on + strand at 4258.303 kb on + strand at 4258.304 kb on - strand at 4258.332 kb on + strand at 4258.336 kb on - strand at 4258.506 kb on + strand, within BPHYT_RS18955 at 4258.519 kb on - strand, within BPHYT_RS18955 at 4258.647 kb on + strand, within BPHYT_RS18955 at 4258.647 kb on + strand, within BPHYT_RS18955 at 4258.648 kb on - strand, within BPHYT_RS18955 at 4258.648 kb on - strand, within BPHYT_RS18955 at 4258.742 kb on - strand, within BPHYT_RS18955 at 4258.742 kb on - strand, within BPHYT_RS18955 at 4258.937 kb on + strand at 4258.937 kb on + strand at 4258.937 kb on + strand at 4258.937 kb on + strand at 4258.938 kb on - strand at 4258.938 kb on - strand at 4258.938 kb on - strand at 4258.938 kb on - strand at 4258.938 kb on - strand at 4258.949 kb on - strand at 4259.046 kb on + strand at 4259.047 kb on - strand at 4259.047 kb on - strand at 4259.047 kb on - strand at 4259.049 kb on + strand at 4259.049 kb on + strand at 4259.049 kb on + strand at 4259.049 kb on + strand at 4259.049 kb on + strand at 4259.050 kb on - strand at 4259.050 kb on - strand at 4259.103 kb on + strand at 4259.103 kb on + strand at 4259.103 kb on + strand at 4259.103 kb on + strand at 4259.103 kb on + strand at 4259.103 kb on + strand at 4259.104 kb on - strand at 4259.104 kb on - strand at 4259.104 kb on - strand at 4259.104 kb on - strand at 4259.388 kb on + strand, within BPHYT_RS18960 at 4259.389 kb on - strand, within BPHYT_RS18960 at 4259.389 kb on - strand, within BPHYT_RS18960 at 4259.688 kb on + strand, within BPHYT_RS18960 at 4259.689 kb on - strand, within BPHYT_RS18960 at 4259.963 kb on - strand, within BPHYT_RS18965 at 4260.128 kb on - strand, within BPHYT_RS18965 at 4260.131 kb on + strand at 4260.365 kb on + strand at 4260.366 kb on - strand at 4260.491 kb on - strand, within BPHYT_RS18970 at 4260.491 kb on - strand, within BPHYT_RS18970 at 4260.520 kb on - strand, within BPHYT_RS18970 at 4260.520 kb on - strand, within BPHYT_RS18970 at 4260.689 kb on - strand, within BPHYT_RS18970 at 4260.689 kb on - strand, within BPHYT_RS18970 at 4260.689 kb on - strand, within BPHYT_RS18970 at 4260.690 kb on + strand, within BPHYT_RS18970 at 4260.690 kb on + strand, within BPHYT_RS18970 at 4260.691 kb on - strand, within BPHYT_RS18970 at 4260.691 kb on - strand, within BPHYT_RS18970 at 4260.691 kb on - strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.784 kb on + strand, within BPHYT_RS18970 at 4260.791 kb on - strand, within BPHYT_RS18970 at 4260.847 kb on + strand, within BPHYT_RS18970 at 4260.847 kb on + strand, within BPHYT_RS18970 at 4260.847 kb on + strand, within BPHYT_RS18970 at 4260.848 kb on - strand, within BPHYT_RS18970 at 4260.848 kb on - strand, within BPHYT_RS18970 at 4260.848 kb on - strand, within BPHYT_RS18970
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 4,258,303 + +0.3 4,258,303 + +1.1 4,258,303 + -0.1 4,258,303 + +0.5 4,258,303 + -0.4 4,258,303 + -0.4 4,258,304 - +1.2 4,258,332 + -0.4 4,258,336 - +0.8 4,258,506 + BPHYT_RS18955 0.32 +0.1 4,258,519 - BPHYT_RS18955 0.34 -0.4 4,258,647 + BPHYT_RS18955 0.57 -1.3 4,258,647 + BPHYT_RS18955 0.57 +0.6 4,258,648 - BPHYT_RS18955 0.58 -0.1 4,258,648 - BPHYT_RS18955 0.58 -0.6 4,258,742 - BPHYT_RS18955 0.75 +0.3 4,258,742 - BPHYT_RS18955 0.75 -0.2 4,258,937 + +0.1 4,258,937 + -1.0 4,258,937 + -0.8 4,258,937 + +0.4 4,258,938 - -1.9 4,258,938 - +2.2 4,258,938 - -1.2 4,258,938 - -0.1 4,258,938 - -1.5 4,258,949 - +1.9 4,259,046 + +0.0 4,259,047 - -0.8 4,259,047 - -0.8 4,259,047 - -0.1 4,259,049 + +0.2 4,259,049 + -1.0 4,259,049 + +0.1 4,259,049 + +1.1 4,259,049 + +0.3 4,259,050 - -0.1 4,259,050 - -0.9 4,259,103 + +1.9 4,259,103 + +0.2 4,259,103 + +0.4 4,259,103 + -0.2 4,259,103 + +1.2 4,259,103 + +2.2 4,259,104 - -0.1 4,259,104 - +0.3 4,259,104 - -0.2 4,259,104 - +1.4 4,259,388 + BPHYT_RS18960 0.31 -1.5 4,259,389 - BPHYT_RS18960 0.31 -1.1 4,259,389 - BPHYT_RS18960 0.31 -1.9 4,259,688 + BPHYT_RS18960 0.73 -1.9 4,259,689 - BPHYT_RS18960 0.73 -2.8 4,259,963 - BPHYT_RS18965 0.21 -1.4 4,260,128 - BPHYT_RS18965 0.63 -0.6 4,260,131 + -0.3 4,260,365 + -0.8 4,260,366 - -0.5 4,260,491 - BPHYT_RS18970 0.12 -2.7 4,260,491 - BPHYT_RS18970 0.12 -3.3 4,260,520 - BPHYT_RS18970 0.15 -3.8 4,260,520 - BPHYT_RS18970 0.15 -1.6 4,260,689 - BPHYT_RS18970 0.30 -3.0 4,260,689 - BPHYT_RS18970 0.30 -2.2 4,260,689 - BPHYT_RS18970 0.30 -2.0 4,260,690 + BPHYT_RS18970 0.30 -2.5 4,260,690 + BPHYT_RS18970 0.30 -2.9 4,260,691 - BPHYT_RS18970 0.30 -1.5 4,260,691 - BPHYT_RS18970 0.30 -5.1 4,260,691 - BPHYT_RS18970 0.30 -3.5 4,260,741 + BPHYT_RS18970 0.35 -1.2 4,260,741 + BPHYT_RS18970 0.35 -2.1 4,260,741 + BPHYT_RS18970 0.35 -2.0 4,260,741 + BPHYT_RS18970 0.35 -2.3 4,260,741 + BPHYT_RS18970 0.35 -2.2 4,260,741 + BPHYT_RS18970 0.35 -1.7 4,260,742 - BPHYT_RS18970 0.35 -3.0 4,260,742 - BPHYT_RS18970 0.35 -0.8 4,260,742 - BPHYT_RS18970 0.35 -5.0 4,260,742 - BPHYT_RS18970 0.35 -1.4 4,260,742 - BPHYT_RS18970 0.35 -2.4 4,260,784 + BPHYT_RS18970 0.39 -0.6 4,260,791 - BPHYT_RS18970 0.39 -1.4 4,260,847 + BPHYT_RS18970 0.45 -3.6 4,260,847 + BPHYT_RS18970 0.45 -3.5 4,260,847 + BPHYT_RS18970 0.45 -2.2 4,260,848 - BPHYT_RS18970 0.45 -2.1 4,260,848 - BPHYT_RS18970 0.45 -4.0 4,260,848 - BPHYT_RS18970 0.45 -3.0
Or see this region's nucleotide sequence