Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18910 and BPHYT_RS18915 are separated by 308 nucleotides BPHYT_RS18915 and BPHYT_RS18920 overlap by 4 nucleotides BPHYT_RS18920 and BPHYT_RS18925 are separated by 60 nucleotides BPHYT_RS18925 and BPHYT_RS18930 are separated by 28 nucleotides BPHYT_RS18930 and BPHYT_RS18935 overlap by 8 nucleotides
BPHYT_RS18910: BPHYT_RS18910 - FlgN, at 4,248,191 to 4,248,637
_RS18910
BPHYT_RS18915: BPHYT_RS18915 - hypothetical protein, at 4,248,946 to 4,249,128
_RS18915
BPHYT_RS18920: BPHYT_RS18920 - hypothetical protein, at 4,249,125 to 4,249,535
_RS18920
BPHYT_RS18925: BPHYT_RS18925 - RNA polymerase sigma70, at 4,249,596 to 4,250,330
_RS18925
BPHYT_RS18930: BPHYT_RS18930 - flagellar biosynthesis protein FlhG, at 4,250,359 to 4,251,228
_RS18930
BPHYT_RS18935: BPHYT_RS18935 - flagellar biosynthesis regulator FlhF, at 4,251,221 to 4,253,146
_RS18935
Position (kb) 4249
4250
4251 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4248.596 kb on + strand at 4248.597 kb on - strand at 4248.775 kb on - strand at 4248.837 kb on + strand at 4248.837 kb on + strand at 4248.838 kb on - strand at 4248.896 kb on + strand at 4248.945 kb on + strand at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.461 kb on + strand, within BPHYT_RS18920 at 4249.738 kb on + strand, within BPHYT_RS18925 at 4249.738 kb on + strand, within BPHYT_RS18925 at 4249.739 kb on - strand, within BPHYT_RS18925 at 4249.903 kb on + strand, within BPHYT_RS18925 at 4249.906 kb on + strand, within BPHYT_RS18925 at 4249.907 kb on - strand, within BPHYT_RS18925 at 4249.907 kb on - strand, within BPHYT_RS18925 at 4249.948 kb on + strand, within BPHYT_RS18925 at 4249.949 kb on - strand, within BPHYT_RS18925 at 4249.949 kb on - strand, within BPHYT_RS18925 at 4250.131 kb on + strand, within BPHYT_RS18925 at 4250.165 kb on - strand, within BPHYT_RS18925 at 4250.281 kb on + strand at 4250.282 kb on - strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.326 kb on + strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.327 kb on - strand at 4250.463 kb on - strand, within BPHYT_RS18930 at 4250.463 kb on - strand, within BPHYT_RS18930 at 4250.577 kb on + strand, within BPHYT_RS18930 at 4250.578 kb on - strand, within BPHYT_RS18930 at 4250.609 kb on + strand, within BPHYT_RS18930 at 4250.647 kb on - strand, within BPHYT_RS18930 at 4250.711 kb on + strand, within BPHYT_RS18930 at 4250.712 kb on - strand, within BPHYT_RS18930 at 4250.712 kb on - strand, within BPHYT_RS18930 at 4250.736 kb on - strand, within BPHYT_RS18930 at 4251.070 kb on + strand, within BPHYT_RS18930 at 4251.222 kb on + strand at 4251.222 kb on + strand at 4251.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 4,248,596 + -2.2 4,248,597 - -1.3 4,248,775 - +0.3 4,248,837 + +1.2 4,248,837 + -0.2 4,248,838 - -0.4 4,248,896 + -0.8 4,248,945 + +0.6 4,249,411 + BPHYT_RS18920 0.70 +1.2 4,249,411 + BPHYT_RS18920 0.70 +1.9 4,249,415 + BPHYT_RS18920 0.71 -0.2 4,249,415 + BPHYT_RS18920 0.71 +1.8 4,249,421 + BPHYT_RS18920 0.72 +0.2 4,249,421 + BPHYT_RS18920 0.72 +0.4 4,249,461 + BPHYT_RS18920 0.82 -2.2 4,249,738 + BPHYT_RS18925 0.19 -1.0 4,249,738 + BPHYT_RS18925 0.19 -0.5 4,249,739 - BPHYT_RS18925 0.19 -1.2 4,249,903 + BPHYT_RS18925 0.42 -2.4 4,249,906 + BPHYT_RS18925 0.42 -2.5 4,249,907 - BPHYT_RS18925 0.42 -1.6 4,249,907 - BPHYT_RS18925 0.42 -0.6 4,249,948 + BPHYT_RS18925 0.48 -1.5 4,249,949 - BPHYT_RS18925 0.48 -1.7 4,249,949 - BPHYT_RS18925 0.48 -1.3 4,250,131 + BPHYT_RS18925 0.73 -1.9 4,250,165 - BPHYT_RS18925 0.77 -1.1 4,250,281 + -0.4 4,250,282 - -2.1 4,250,326 + -0.7 4,250,326 + -2.0 4,250,326 + -2.1 4,250,326 + -1.7 4,250,326 + -1.7 4,250,327 - -1.8 4,250,327 - -1.4 4,250,327 - -1.4 4,250,327 - -1.3 4,250,463 - BPHYT_RS18930 0.12 -2.9 4,250,463 - BPHYT_RS18930 0.12 -1.5 4,250,577 + BPHYT_RS18930 0.25 -2.9 4,250,578 - BPHYT_RS18930 0.25 -0.4 4,250,609 + BPHYT_RS18930 0.29 -1.9 4,250,647 - BPHYT_RS18930 0.33 -2.6 4,250,711 + BPHYT_RS18930 0.40 -3.5 4,250,712 - BPHYT_RS18930 0.41 +0.7 4,250,712 - BPHYT_RS18930 0.41 +0.1 4,250,736 - BPHYT_RS18930 0.43 -2.0 4,251,070 + BPHYT_RS18930 0.82 -1.6 4,251,222 + -2.2 4,251,222 + -1.7 4,251,223 - +3.3
Or see this region's nucleotide sequence