Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18865 and BPHYT_RS18870 are separated by 36 nucleotides BPHYT_RS18870 and BPHYT_RS18875 are separated by 12 nucleotides BPHYT_RS18875 and BPHYT_RS18880 are separated by 20 nucleotides
BPHYT_RS18865: BPHYT_RS18865 - flagellar L-ring protein FlgH, at 4,240,194 to 4,240,892
_RS18865
BPHYT_RS18870: BPHYT_RS18870 - flagellar basal body rod protein FlgG, at 4,240,929 to 4,241,717
_RS18870
BPHYT_RS18875: BPHYT_RS18875 - flagellar basal body rod protein FlgF, at 4,241,730 to 4,242,488
_RS18875
BPHYT_RS18880: BPHYT_RS18880 - flagellar hook protein FlgE, at 4,242,509 to 4,244,020
_RS18880
Position (kb)
4241
4242
4243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4240.875 kb on - strand at 4240.909 kb on + strand at 4240.930 kb on + strand at 4240.930 kb on + strand at 4240.930 kb on + strand at 4240.931 kb on - strand at 4241.000 kb on - strand at 4241.065 kb on + strand, within BPHYT_RS18870 at 4241.065 kb on + strand, within BPHYT_RS18870 at 4241.066 kb on - strand, within BPHYT_RS18870 at 4241.378 kb on - strand, within BPHYT_RS18870 at 4241.476 kb on + strand, within BPHYT_RS18870 at 4241.477 kb on - strand, within BPHYT_RS18870 at 4241.582 kb on - strand, within BPHYT_RS18870 at 4241.701 kb on + strand at 4241.701 kb on + strand at 4241.701 kb on + strand at 4241.702 kb on - strand at 4241.735 kb on + strand at 4241.736 kb on - strand at 4241.736 kb on - strand at 4242.175 kb on + strand, within BPHYT_RS18875 at 4242.176 kb on - strand, within BPHYT_RS18875 at 4242.274 kb on + strand, within BPHYT_RS18875 at 4242.274 kb on + strand, within BPHYT_RS18875 at 4242.275 kb on - strand, within BPHYT_RS18875 at 4242.511 kb on - strand at 4242.511 kb on - strand at 4242.574 kb on - strand at 4242.574 kb on - strand at 4242.574 kb on - strand at 4242.909 kb on + strand, within BPHYT_RS18880 at 4242.909 kb on + strand, within BPHYT_RS18880 at 4242.909 kb on + strand, within BPHYT_RS18880 at 4242.910 kb on - strand, within BPHYT_RS18880 at 4242.987 kb on + strand, within BPHYT_RS18880 at 4242.987 kb on + strand, within BPHYT_RS18880 at 4242.987 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.140 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.176 kb on + strand, within BPHYT_RS18880 at 4243.197 kb on + strand, within BPHYT_RS18880 at 4243.198 kb on - strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.203 kb on + strand, within BPHYT_RS18880 at 4243.204 kb on - strand, within BPHYT_RS18880 at 4243.333 kb on - strand, within BPHYT_RS18880 at 4243.410 kb on + strand, within BPHYT_RS18880 at 4243.410 kb on + strand, within BPHYT_RS18880 at 4243.411 kb on - strand, within BPHYT_RS18880 at 4243.411 kb on - strand, within BPHYT_RS18880
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 4,240,875 - -2.0 4,240,909 + -1.3 4,240,930 + -0.6 4,240,930 + -0.6 4,240,930 + +5.1 4,240,931 - +1.3 4,241,000 - -1.2 4,241,065 + BPHYT_RS18870 0.17 -0.6 4,241,065 + BPHYT_RS18870 0.17 -1.9 4,241,066 - BPHYT_RS18870 0.17 -3.3 4,241,378 - BPHYT_RS18870 0.57 -0.9 4,241,476 + BPHYT_RS18870 0.69 -2.4 4,241,477 - BPHYT_RS18870 0.69 -0.8 4,241,582 - BPHYT_RS18870 0.83 -1.1 4,241,701 + +0.3 4,241,701 + -3.4 4,241,701 + -0.4 4,241,702 - -2.2 4,241,735 + -3.6 4,241,736 - +0.2 4,241,736 - -1.0 4,242,175 + BPHYT_RS18875 0.59 -1.2 4,242,176 - BPHYT_RS18875 0.59 -2.9 4,242,274 + BPHYT_RS18875 0.72 -0.2 4,242,274 + BPHYT_RS18875 0.72 -1.5 4,242,275 - BPHYT_RS18875 0.72 -1.2 4,242,511 - -0.1 4,242,511 - -2.5 4,242,574 - -1.4 4,242,574 - +0.1 4,242,574 - -0.8 4,242,909 + BPHYT_RS18880 0.26 -1.2 4,242,909 + BPHYT_RS18880 0.26 -0.4 4,242,909 + BPHYT_RS18880 0.26 -0.1 4,242,910 - BPHYT_RS18880 0.27 -0.5 4,242,987 + BPHYT_RS18880 0.32 -1.1 4,242,987 + BPHYT_RS18880 0.32 -1.7 4,242,987 + BPHYT_RS18880 0.32 +0.4 4,243,140 + BPHYT_RS18880 0.42 +0.5 4,243,140 + BPHYT_RS18880 0.42 -1.5 4,243,140 + BPHYT_RS18880 0.42 -1.0 4,243,176 + BPHYT_RS18880 0.44 -0.6 4,243,176 + BPHYT_RS18880 0.44 -0.2 4,243,176 + BPHYT_RS18880 0.44 -0.9 4,243,197 + BPHYT_RS18880 0.46 +0.7 4,243,198 - BPHYT_RS18880 0.46 -3.4 4,243,203 + BPHYT_RS18880 0.46 +1.2 4,243,203 + BPHYT_RS18880 0.46 -0.7 4,243,203 + BPHYT_RS18880 0.46 -2.9 4,243,204 - BPHYT_RS18880 0.46 -2.5 4,243,333 - BPHYT_RS18880 0.54 -1.2 4,243,410 + BPHYT_RS18880 0.60 -0.5 4,243,410 + BPHYT_RS18880 0.60 -0.9 4,243,411 - BPHYT_RS18880 0.60 -0.1 4,243,411 - BPHYT_RS18880 0.60 -3.2
Or see this region's nucleotide sequence