Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18855 and BPHYT_RS18860 are separated by 12 nucleotides BPHYT_RS18860 and BPHYT_RS18865 are separated by 2 nucleotides BPHYT_RS18865 and BPHYT_RS18870 are separated by 36 nucleotides
BPHYT_RS18855: BPHYT_RS18855 - flagellar rod assembly protein FlgJ, at 4,237,993 to 4,238,991
_RS18855
BPHYT_RS18860: BPHYT_RS18860 - flagellar P-ring protein FlgI, at 4,239,004 to 4,240,191
_RS18860
BPHYT_RS18865: BPHYT_RS18865 - flagellar L-ring protein FlgH, at 4,240,194 to 4,240,892
_RS18865
BPHYT_RS18870: BPHYT_RS18870 - flagellar basal body rod protein FlgG, at 4,240,929 to 4,241,717
_RS18870
Position (kb) 4239
4240
4241 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 4238.034 kb on - strand at 4238.148 kb on - strand, within BPHYT_RS18855 at 4238.169 kb on - strand, within BPHYT_RS18855 at 4238.223 kb on - strand, within BPHYT_RS18855 at 4238.223 kb on - strand, within BPHYT_RS18855 at 4238.223 kb on - strand, within BPHYT_RS18855 at 4238.422 kb on - strand, within BPHYT_RS18855 at 4238.496 kb on - strand, within BPHYT_RS18855 at 4238.653 kb on + strand, within BPHYT_RS18855 at 4238.654 kb on - strand, within BPHYT_RS18855 at 4238.980 kb on - strand at 4239.005 kb on + strand at 4239.005 kb on + strand at 4239.005 kb on + strand at 4239.005 kb on + strand at 4239.006 kb on - strand at 4239.006 kb on - strand at 4239.006 kb on - strand at 4239.088 kb on + strand at 4239.089 kb on - strand at 4239.089 kb on - strand at 4239.244 kb on - strand, within BPHYT_RS18860 at 4239.275 kb on - strand, within BPHYT_RS18860 at 4239.518 kb on + strand, within BPHYT_RS18860 at 4239.519 kb on - strand, within BPHYT_RS18860 at 4239.698 kb on + strand, within BPHYT_RS18860 at 4239.699 kb on - strand, within BPHYT_RS18860 at 4239.699 kb on - strand, within BPHYT_RS18860 at 4239.699 kb on - strand, within BPHYT_RS18860 at 4240.080 kb on - strand at 4240.185 kb on + strand at 4240.186 kb on - strand at 4240.238 kb on + strand at 4240.256 kb on + strand at 4240.258 kb on + strand at 4240.259 kb on - strand at 4240.259 kb on - strand at 4240.259 kb on - strand at 4240.307 kb on - strand, within BPHYT_RS18865 at 4240.307 kb on - strand, within BPHYT_RS18865 at 4240.717 kb on + strand, within BPHYT_RS18865 at 4240.875 kb on - strand at 4240.909 kb on + strand at 4240.930 kb on + strand at 4240.930 kb on + strand at 4240.930 kb on + strand at 4240.931 kb on - strand at 4241.000 kb on - strand at 4241.065 kb on + strand, within BPHYT_RS18870 at 4241.065 kb on + strand, within BPHYT_RS18870 at 4241.066 kb on - strand, within BPHYT_RS18870
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 4,238,034 - +0.3 4,238,148 - BPHYT_RS18855 0.16 +0.2 4,238,169 - BPHYT_RS18855 0.18 -0.6 4,238,223 - BPHYT_RS18855 0.23 -1.7 4,238,223 - BPHYT_RS18855 0.23 -0.0 4,238,223 - BPHYT_RS18855 0.23 -0.9 4,238,422 - BPHYT_RS18855 0.43 +0.0 4,238,496 - BPHYT_RS18855 0.50 -1.6 4,238,653 + BPHYT_RS18855 0.66 +0.4 4,238,654 - BPHYT_RS18855 0.66 -0.4 4,238,980 - -0.2 4,239,005 + -0.9 4,239,005 + +0.4 4,239,005 + -0.8 4,239,005 + -3.6 4,239,006 - +0.3 4,239,006 - -1.0 4,239,006 - -0.4 4,239,088 + -0.7 4,239,089 - -1.5 4,239,089 - -0.8 4,239,244 - BPHYT_RS18860 0.20 -0.2 4,239,275 - BPHYT_RS18860 0.23 -1.8 4,239,518 + BPHYT_RS18860 0.43 -0.3 4,239,519 - BPHYT_RS18860 0.43 -0.5 4,239,698 + BPHYT_RS18860 0.58 -4.6 4,239,699 - BPHYT_RS18860 0.59 -2.4 4,239,699 - BPHYT_RS18860 0.59 -1.2 4,239,699 - BPHYT_RS18860 0.59 -1.0 4,240,080 - +0.7 4,240,185 + -1.4 4,240,186 - -1.1 4,240,238 + -1.8 4,240,256 + -1.4 4,240,258 + +1.8 4,240,259 - -0.6 4,240,259 - -0.1 4,240,259 - +0.9 4,240,307 - BPHYT_RS18865 0.16 -0.7 4,240,307 - BPHYT_RS18865 0.16 -2.1 4,240,717 + BPHYT_RS18865 0.75 -0.7 4,240,875 - -2.0 4,240,909 + -1.3 4,240,930 + -0.6 4,240,930 + -0.6 4,240,930 + +5.1 4,240,931 - +1.3 4,241,000 - -1.2 4,241,065 + BPHYT_RS18870 0.17 -0.6 4,241,065 + BPHYT_RS18870 0.17 -1.9 4,241,066 - BPHYT_RS18870 0.17 -3.3
Or see this region's nucleotide sequence