Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18810 and BPHYT_RS18815 overlap by 8 nucleotides BPHYT_RS18815 and BPHYT_RS18820 are separated by 92 nucleotides BPHYT_RS18820 and BPHYT_RS18825 overlap by 1 nucleotides
BPHYT_RS18810: BPHYT_RS18810 - flagellar motor switch protein FliM, at 4,229,273 to 4,230,271
_RS18810
BPHYT_RS18815: BPHYT_RS18815 - flagellar motor switch protein FliN, at 4,230,264 to 4,230,728
_RS18815
BPHYT_RS18820: BPHYT_RS18820 - flagellar biosynthesis protein FliO, at 4,230,821 to 4,231,336
_RS18820
BPHYT_RS18825: BPHYT_RS18825 - flagellar biosynthesis protein FliP, at 4,231,336 to 4,232,169
_RS18825
Position (kb)
4230
4231 Strain fitness (log2 ratio)
-2
-1
0
1 at 4229.267 kb on + strand at 4229.268 kb on - strand at 4229.268 kb on - strand at 4229.268 kb on - strand at 4229.268 kb on - strand at 4229.385 kb on - strand, within BPHYT_RS18810 at 4229.537 kb on + strand, within BPHYT_RS18810 at 4229.617 kb on - strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.765 kb on + strand, within BPHYT_RS18810 at 4229.766 kb on - strand, within BPHYT_RS18810 at 4229.766 kb on - strand, within BPHYT_RS18810 at 4229.917 kb on + strand, within BPHYT_RS18810 at 4229.917 kb on + strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4230.207 kb on - strand at 4230.263 kb on + strand at 4230.264 kb on - strand at 4230.264 kb on - strand at 4230.692 kb on + strand at 4230.693 kb on - strand at 4230.824 kb on + strand at 4230.825 kb on - strand at 4231.115 kb on - strand, within BPHYT_RS18820 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.547 kb on - strand, within BPHYT_RS18825
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 4,229,267 + -0.2 4,229,268 - -0.5 4,229,268 - -0.7 4,229,268 - -0.1 4,229,268 - -0.7 4,229,385 - BPHYT_RS18810 0.11 -2.2 4,229,537 + BPHYT_RS18810 0.26 +0.2 4,229,617 - BPHYT_RS18810 0.34 -1.0 4,229,765 + BPHYT_RS18810 0.49 -1.0 4,229,765 + BPHYT_RS18810 0.49 -1.5 4,229,765 + BPHYT_RS18810 0.49 +0.1 4,229,765 + BPHYT_RS18810 0.49 +0.0 4,229,765 + BPHYT_RS18810 0.49 +1.3 4,229,766 - BPHYT_RS18810 0.49 -1.4 4,229,766 - BPHYT_RS18810 0.49 -1.9 4,229,917 + BPHYT_RS18810 0.64 +0.1 4,229,917 + BPHYT_RS18810 0.64 -1.5 4,229,918 - BPHYT_RS18810 0.65 +0.8 4,229,918 - BPHYT_RS18810 0.65 +0.0 4,229,918 - BPHYT_RS18810 0.65 -2.6 4,229,918 - BPHYT_RS18810 0.65 -1.3 4,229,918 - BPHYT_RS18810 0.65 -0.9 4,230,207 - -0.2 4,230,263 + -0.1 4,230,264 - -1.0 4,230,264 - +0.1 4,230,692 + -0.9 4,230,693 - -0.4 4,230,824 + -0.3 4,230,825 - -1.6 4,231,115 - BPHYT_RS18820 0.57 -1.5 4,231,284 + BPHYT_RS18820 0.90 +0.8 4,231,284 + BPHYT_RS18820 0.90 -1.8 4,231,547 - BPHYT_RS18825 0.25 -0.3
Or see this region's nucleotide sequence