Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18140

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18130 and BPHYT_RS18135 are separated by 134 nucleotidesBPHYT_RS18135 and BPHYT_RS18140 are separated by 178 nucleotidesBPHYT_RS18140 and BPHYT_RS18145 are separated by 222 nucleotides BPHYT_RS18130: BPHYT_RS18130 - elongation factor G, at 4,076,716 to 4,078,818 _RS18130 BPHYT_RS18135: BPHYT_RS18135 - 30S ribosomal protein S7, at 4,078,953 to 4,079,423 _RS18135 BPHYT_RS18140: BPHYT_RS18140 - 30S ribosomal protein S12, at 4,079,602 to 4,079,982 _RS18140 BPHYT_RS18145: BPHYT_RS18145 - ATP-dependent DNA helicase RecQ, at 4,080,205 to 4,082,052 _RS18145 Position (kb) 4079 4080Strain fitness (log2 ratio) -2 -1 0 1at 4080.350 kb on + strandat 4080.350 kb on + strandat 4080.489 kb on + strand, within BPHYT_RS18145at 4080.490 kb on - strand, within BPHYT_RS18145at 4080.500 kb on + strand, within BPHYT_RS18145at 4080.500 kb on + strand, within BPHYT_RS18145at 4080.733 kb on + strand, within BPHYT_RS18145at 4080.733 kb on + strand, within BPHYT_RS18145at 4080.734 kb on - strand, within BPHYT_RS18145at 4080.734 kb on - strand, within BPHYT_RS18145at 4080.884 kb on + strand, within BPHYT_RS18145

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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4,080,350 + -0.3
4,080,350 + +0.7
4,080,489 + BPHYT_RS18145 0.15 +1.3
4,080,490 - BPHYT_RS18145 0.15 -1.0
4,080,500 + BPHYT_RS18145 0.16 -1.9
4,080,500 + BPHYT_RS18145 0.16 -0.0
4,080,733 + BPHYT_RS18145 0.29 +0.4
4,080,733 + BPHYT_RS18145 0.29 +0.0
4,080,734 - BPHYT_RS18145 0.29 -0.6
4,080,734 - BPHYT_RS18145 0.29 +1.5
4,080,884 + BPHYT_RS18145 0.37 -0.4

Or see this region's nucleotide sequence