Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17685 and BPHYT_RS17690 are separated by 229 nucleotides BPHYT_RS17690 and BPHYT_RS17695 overlap by 4 nucleotides BPHYT_RS17695 and BPHYT_RS17700 are separated by 79 nucleotides
BPHYT_RS17685: BPHYT_RS17685 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 3,996,480 to 3,997,232
_RS17685
BPHYT_RS17690: BPHYT_RS17690 - imidazole glycerol phosphate synthase, at 3,997,462 to 3,998,103
_RS17690
BPHYT_RS17695: BPHYT_RS17695 - membrane protein, at 3,998,100 to 3,998,720
_RS17695
BPHYT_RS17700: BPHYT_RS17700 - imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) (from data) , at 3,998,800 to 3,999,387
_RS17700
Position (kb)
3997
3998
3999 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 3996.482 kb on - strand at 3996.482 kb on - strand at 3996.733 kb on + strand, within BPHYT_RS17685 at 3996.754 kb on + strand, within BPHYT_RS17685 at 3996.755 kb on - strand, within BPHYT_RS17685 at 3996.857 kb on + strand, within BPHYT_RS17685 at 3996.857 kb on + strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.952 kb on + strand, within BPHYT_RS17685 at 3996.953 kb on - strand, within BPHYT_RS17685 at 3997.188 kb on + strand at 3997.188 kb on + strand at 3997.188 kb on + strand at 3997.189 kb on - strand at 3997.232 kb on + strand at 3997.232 kb on + strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.334 kb on - strand at 3997.337 kb on - strand at 3997.337 kb on - strand at 3997.387 kb on + strand at 3997.387 kb on + strand at 3997.388 kb on - strand at 3997.410 kb on + strand at 3997.411 kb on - strand at 3997.411 kb on - strand at 3997.411 kb on - strand at 3997.548 kb on + strand, within BPHYT_RS17690 at 3997.583 kb on + strand, within BPHYT_RS17690 at 3997.583 kb on + strand, within BPHYT_RS17690 at 3997.583 kb on + strand, within BPHYT_RS17690 at 3997.583 kb on + strand, within BPHYT_RS17690 at 3997.628 kb on + strand, within BPHYT_RS17690 at 3997.628 kb on + strand, within BPHYT_RS17690 at 3997.628 kb on + strand, within BPHYT_RS17690 at 3997.628 kb on + strand, within BPHYT_RS17690 at 3997.629 kb on - strand, within BPHYT_RS17690 at 3997.629 kb on - strand, within BPHYT_RS17690 at 3997.629 kb on - strand, within BPHYT_RS17690 at 3997.631 kb on + strand, within BPHYT_RS17690 at 3997.632 kb on - strand, within BPHYT_RS17690 at 3997.646 kb on + strand, within BPHYT_RS17690 at 3998.112 kb on - strand at 3998.199 kb on - strand, within BPHYT_RS17695 at 3998.199 kb on - strand, within BPHYT_RS17695 at 3998.302 kb on + strand, within BPHYT_RS17695 at 3998.303 kb on - strand, within BPHYT_RS17695 at 3998.303 kb on - strand, within BPHYT_RS17695 at 3998.303 kb on - strand, within BPHYT_RS17695 at 3998.303 kb on - strand, within BPHYT_RS17695 at 3998.327 kb on - strand, within BPHYT_RS17695 at 3998.612 kb on + strand, within BPHYT_RS17695 at 3998.612 kb on + strand, within BPHYT_RS17695 at 3998.613 kb on - strand, within BPHYT_RS17695 at 3998.613 kb on - strand, within BPHYT_RS17695 at 3998.613 kb on - strand, within BPHYT_RS17695 at 3998.699 kb on + strand at 3998.700 kb on - strand at 3998.765 kb on + strand at 3998.779 kb on + strand at 3998.802 kb on - strand at 3998.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,996,482 - -0.6 3,996,482 - -0.2 3,996,733 + BPHYT_RS17685 0.34 -2.4 3,996,754 + BPHYT_RS17685 0.36 -6.9 3,996,755 - BPHYT_RS17685 0.37 -3.6 3,996,857 + BPHYT_RS17685 0.50 -3.4 3,996,857 + BPHYT_RS17685 0.50 -3.3 3,996,932 - BPHYT_RS17685 0.60 -6.5 3,996,932 - BPHYT_RS17685 0.60 -4.0 3,996,932 - BPHYT_RS17685 0.60 -4.0 3,996,952 + BPHYT_RS17685 0.63 -4.5 3,996,953 - BPHYT_RS17685 0.63 -4.7 3,997,188 + -5.6 3,997,188 + -4.7 3,997,188 + -1.9 3,997,189 - -4.4 3,997,232 + -3.8 3,997,232 + -5.2 3,997,233 - -3.6 3,997,233 - -3.7 3,997,233 - -5.0 3,997,233 - -5.4 3,997,233 - -2.7 3,997,334 - +1.8 3,997,337 - -0.8 3,997,337 - -0.2 3,997,387 + -0.4 3,997,387 + -0.5 3,997,388 - +0.4 3,997,410 + -3.0 3,997,411 - +0.5 3,997,411 - +0.3 3,997,411 - +1.1 3,997,548 + BPHYT_RS17690 0.13 -7.2 3,997,583 + BPHYT_RS17690 0.19 -4.7 3,997,583 + BPHYT_RS17690 0.19 -3.9 3,997,583 + BPHYT_RS17690 0.19 -6.2 3,997,583 + BPHYT_RS17690 0.19 -3.6 3,997,628 + BPHYT_RS17690 0.26 -2.9 3,997,628 + BPHYT_RS17690 0.26 -4.0 3,997,628 + BPHYT_RS17690 0.26 -7.1 3,997,628 + BPHYT_RS17690 0.26 -4.7 3,997,629 - BPHYT_RS17690 0.26 -3.1 3,997,629 - BPHYT_RS17690 0.26 -1.2 3,997,629 - BPHYT_RS17690 0.26 -4.6 3,997,631 + BPHYT_RS17690 0.26 -5.9 3,997,632 - BPHYT_RS17690 0.26 -2.1 3,997,646 + BPHYT_RS17690 0.29 -5.0 3,998,112 - -1.5 3,998,199 - BPHYT_RS17695 0.16 -0.4 3,998,199 - BPHYT_RS17695 0.16 -0.9 3,998,302 + BPHYT_RS17695 0.33 -1.7 3,998,303 - BPHYT_RS17695 0.33 +0.8 3,998,303 - BPHYT_RS17695 0.33 -0.8 3,998,303 - BPHYT_RS17695 0.33 +1.3 3,998,303 - BPHYT_RS17695 0.33 +0.1 3,998,327 - BPHYT_RS17695 0.37 -0.4 3,998,612 + BPHYT_RS17695 0.82 -3.3 3,998,612 + BPHYT_RS17695 0.82 -2.5 3,998,613 - BPHYT_RS17695 0.83 -2.7 3,998,613 - BPHYT_RS17695 0.83 -0.9 3,998,613 - BPHYT_RS17695 0.83 -0.1 3,998,699 + -5.7 3,998,700 - -0.7 3,998,765 + -4.1 3,998,779 + -2.2 3,998,802 - -0.6 3,998,802 - -0.7
Or see this region's nucleotide sequence