Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17650 and BPHYT_RS17655 are separated by 39 nucleotides BPHYT_RS17655 and BPHYT_RS17660 are separated by 237 nucleotides BPHYT_RS17660 and BPHYT_RS17665 are separated by 81 nucleotides BPHYT_RS17665 and BPHYT_RS17670 are separated by 94 nucleotides BPHYT_RS17670 and BPHYT_RS17675 overlap by 4 nucleotides
BPHYT_RS17650: BPHYT_RS17650 - translocase, at 3,992,693 to 3,993,211
_RS17650
BPHYT_RS17655: BPHYT_RS17655 - Sec-independent protein translocase protein TatA, at 3,993,251 to 3,993,490
_RS17655
BPHYT_RS17660: BPHYT_RS17660 - HIT family hydrolase, at 3,993,728 to 3,994,093
_RS17660
BPHYT_RS17665: BPHYT_RS17665 - membrane protein, at 3,994,175 to 3,994,570
_RS17665
BPHYT_RS17670: BPHYT_RS17670 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 3,994,665 to 3,995,060
_RS17670
BPHYT_RS17675: BPHYT_RS17675 - phosphoribosyl-AMP cyclohydrolase, at 3,995,057 to 3,995,464
_RS17675
Position (kb)
3993
3994
3995 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3993.533 kb on - strand at 3993.578 kb on - strand at 3993.578 kb on - strand at 3993.578 kb on - strand at 3993.578 kb on - strand at 3993.578 kb on - strand at 3993.580 kb on - strand at 3993.580 kb on - strand at 3993.580 kb on - strand at 3993.580 kb on - strand at 3993.580 kb on - strand at 3993.580 kb on - strand at 3993.656 kb on - strand at 3993.656 kb on - strand at 3993.656 kb on - strand at 3993.784 kb on - strand, within BPHYT_RS17660 at 3993.784 kb on - strand, within BPHYT_RS17660 at 3993.819 kb on - strand, within BPHYT_RS17660 at 3993.899 kb on - strand, within BPHYT_RS17660 at 3993.899 kb on - strand, within BPHYT_RS17660 at 3993.899 kb on - strand, within BPHYT_RS17660 at 3994.096 kb on + strand at 3994.097 kb on - strand at 3994.097 kb on - strand at 3994.111 kb on - strand at 3994.111 kb on - strand at 3994.111 kb on - strand at 3994.211 kb on - strand at 3994.215 kb on + strand, within BPHYT_RS17665 at 3994.216 kb on - strand, within BPHYT_RS17665 at 3994.216 kb on - strand, within BPHYT_RS17665 at 3994.216 kb on - strand, within BPHYT_RS17665 at 3994.221 kb on + strand, within BPHYT_RS17665 at 3994.222 kb on - strand, within BPHYT_RS17665 at 3994.238 kb on - strand, within BPHYT_RS17665 at 3994.238 kb on - strand, within BPHYT_RS17665 at 3994.238 kb on - strand, within BPHYT_RS17665 at 3994.240 kb on + strand, within BPHYT_RS17665 at 3994.240 kb on + strand, within BPHYT_RS17665 at 3994.240 kb on + strand, within BPHYT_RS17665 at 3994.240 kb on + strand, within BPHYT_RS17665 at 3994.241 kb on - strand, within BPHYT_RS17665 at 3994.245 kb on + strand, within BPHYT_RS17665 at 3994.245 kb on + strand, within BPHYT_RS17665 at 3994.375 kb on - strand, within BPHYT_RS17665 at 3994.403 kb on + strand, within BPHYT_RS17665 at 3994.403 kb on + strand, within BPHYT_RS17665 at 3994.404 kb on - strand, within BPHYT_RS17665 at 3994.404 kb on - strand, within BPHYT_RS17665 at 3994.404 kb on - strand, within BPHYT_RS17665 at 3994.407 kb on + strand, within BPHYT_RS17665 at 3994.408 kb on - strand, within BPHYT_RS17665 at 3994.408 kb on - strand, within BPHYT_RS17665 at 3994.408 kb on - strand, within BPHYT_RS17665 at 3994.408 kb on - strand, within BPHYT_RS17665 at 3994.464 kb on + strand, within BPHYT_RS17665 at 3994.464 kb on + strand, within BPHYT_RS17665 at 3994.464 kb on + strand, within BPHYT_RS17665 at 3994.473 kb on + strand, within BPHYT_RS17665 at 3994.492 kb on + strand, within BPHYT_RS17665 at 3994.549 kb on - strand at 3994.660 kb on + strand at 3994.948 kb on + strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3995.009 kb on + strand, within BPHYT_RS17670 at 3995.009 kb on + strand, within BPHYT_RS17670 at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,993,533 - -0.3 3,993,578 - -0.3 3,993,578 - +0.6 3,993,578 - -0.2 3,993,578 - -1.1 3,993,578 - -1.2 3,993,580 - +0.9 3,993,580 - -0.2 3,993,580 - -0.0 3,993,580 - -0.5 3,993,580 - -0.8 3,993,580 - +0.6 3,993,656 - +0.3 3,993,656 - +1.8 3,993,656 - +1.2 3,993,784 - BPHYT_RS17660 0.15 -0.1 3,993,784 - BPHYT_RS17660 0.15 -0.4 3,993,819 - BPHYT_RS17660 0.25 -0.2 3,993,899 - BPHYT_RS17660 0.47 +0.2 3,993,899 - BPHYT_RS17660 0.47 -0.6 3,993,899 - BPHYT_RS17660 0.47 +0.9 3,994,096 + +0.5 3,994,097 - -0.7 3,994,097 - -2.1 3,994,111 - +0.2 3,994,111 - -0.6 3,994,111 - +0.3 3,994,211 - -0.9 3,994,215 + BPHYT_RS17665 0.10 -0.2 3,994,216 - BPHYT_RS17665 0.10 +1.1 3,994,216 - BPHYT_RS17665 0.10 +0.0 3,994,216 - BPHYT_RS17665 0.10 -0.4 3,994,221 + BPHYT_RS17665 0.12 -0.1 3,994,222 - BPHYT_RS17665 0.12 +0.1 3,994,238 - BPHYT_RS17665 0.16 -3.1 3,994,238 - BPHYT_RS17665 0.16 +0.0 3,994,238 - BPHYT_RS17665 0.16 -2.0 3,994,240 + BPHYT_RS17665 0.16 -1.7 3,994,240 + BPHYT_RS17665 0.16 +2.4 3,994,240 + BPHYT_RS17665 0.16 +0.3 3,994,240 + BPHYT_RS17665 0.16 +1.6 3,994,241 - BPHYT_RS17665 0.17 +0.2 3,994,245 + BPHYT_RS17665 0.18 -0.9 3,994,245 + BPHYT_RS17665 0.18 -0.1 3,994,375 - BPHYT_RS17665 0.51 +0.8 3,994,403 + BPHYT_RS17665 0.58 -0.5 3,994,403 + BPHYT_RS17665 0.58 +0.5 3,994,404 - BPHYT_RS17665 0.58 -0.2 3,994,404 - BPHYT_RS17665 0.58 +1.0 3,994,404 - BPHYT_RS17665 0.58 -0.6 3,994,407 + BPHYT_RS17665 0.59 +0.3 3,994,408 - BPHYT_RS17665 0.59 +0.4 3,994,408 - BPHYT_RS17665 0.59 +0.3 3,994,408 - BPHYT_RS17665 0.59 -2.2 3,994,408 - BPHYT_RS17665 0.59 +0.0 3,994,464 + BPHYT_RS17665 0.73 -1.3 3,994,464 + BPHYT_RS17665 0.73 -1.1 3,994,464 + BPHYT_RS17665 0.73 +0.7 3,994,473 + BPHYT_RS17665 0.75 -1.9 3,994,492 + BPHYT_RS17665 0.80 +0.2 3,994,549 - +0.2 3,994,660 + +0.8 3,994,948 + BPHYT_RS17670 0.71 -3.6 3,994,949 - BPHYT_RS17670 0.72 -4.6 3,994,949 - BPHYT_RS17670 0.72 +0.3 3,994,949 - BPHYT_RS17670 0.72 -4.2 3,995,009 + BPHYT_RS17670 0.87 -1.8 3,995,009 + BPHYT_RS17670 0.87 -4.3 3,995,064 + -3.7 3,995,064 + -5.7 3,995,064 + -1.5 3,995,064 + -1.4 3,995,064 + -2.1 3,995,065 - +1.6 3,995,065 - -0.0 3,995,065 - -1.1 3,995,065 - +1.2
Or see this region's nucleotide sequence