Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS17410

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS17400 and BPHYT_RS17405 are separated by 30 nucleotidesBPHYT_RS17405 and BPHYT_RS17410 are separated by 46 nucleotidesBPHYT_RS17410 and BPHYT_RS17415 are separated by 84 nucleotides BPHYT_RS17400: BPHYT_RS17400 - anthranilate phosphoribosyltransferase, at 3,942,611 to 3,943,642 _RS17400 BPHYT_RS17405: BPHYT_RS17405 - indole-3-glycerol phosphate synthase, at 3,943,673 to 3,944,458 _RS17405 BPHYT_RS17410: BPHYT_RS17410 - adenylate cyclase, at 3,944,505 to 3,945,131 _RS17410 BPHYT_RS17415: BPHYT_RS17415 - uracil-DNA glycosylase, at 3,945,216 to 3,946,061 _RS17415 Position (kb) 3944 3945 3946Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1 2at 3943.517 kb on + strand, within BPHYT_RS17400at 3943.518 kb on - strand, within BPHYT_RS17400at 3943.518 kb on - strand, within BPHYT_RS17400at 3943.648 kb on + strandat 3943.661 kb on - strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.672 kb on + strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.673 kb on - strandat 3943.694 kb on - strandat 3943.985 kb on + strand, within BPHYT_RS17405at 3944.492 kb on - strandat 3944.492 kb on - strandat 3944.618 kb on + strand, within BPHYT_RS17410at 3944.622 kb on + strand, within BPHYT_RS17410at 3944.623 kb on - strand, within BPHYT_RS17410at 3945.067 kb on + strand, within BPHYT_RS17410at 3945.067 kb on + strand, within BPHYT_RS17410at 3945.112 kb on - strandat 3946.059 kb on + strandat 3946.059 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,943,517 + BPHYT_RS17400 0.88 -4.6
3,943,518 - BPHYT_RS17400 0.88 -3.5
3,943,518 - BPHYT_RS17400 0.88 -6.0
3,943,648 + +0.7
3,943,661 - -5.9
3,943,672 + -0.5
3,943,672 + -0.4
3,943,672 + -0.0
3,943,672 + -0.1
3,943,672 + +0.1
3,943,673 - -3.9
3,943,673 - -7.7
3,943,673 - -3.3
3,943,673 - -1.0
3,943,673 - -3.6
3,943,694 - -5.3
3,943,985 + BPHYT_RS17405 0.40 -3.9
3,944,492 - -1.2
3,944,492 - +2.1
3,944,618 + BPHYT_RS17410 0.18 -1.0
3,944,622 + BPHYT_RS17410 0.19 -1.9
3,944,623 - BPHYT_RS17410 0.19 -0.7
3,945,067 + BPHYT_RS17410 0.90 -0.4
3,945,067 + BPHYT_RS17410 0.90 +0.3
3,945,112 - -1.0
3,946,059 + +1.5
3,946,059 + -0.5

Or see this region's nucleotide sequence