Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17060 and BPHYT_RS17065 are separated by 551 nucleotides BPHYT_RS17065 and BPHYT_RS17070 overlap by 4 nucleotides BPHYT_RS17070 and BPHYT_RS17075 are separated by 9 nucleotides
BPHYT_RS17060: BPHYT_RS17060 - ribonucleotide-diphosphate reductase subunit alpha, at 3,866,297 to 3,869,302
_RS17060
BPHYT_RS17065: BPHYT_RS17065 - N-acetyl-anhydromuranmyl-L-alanine amidase, at 3,869,854 to 3,870,450
_RS17065
BPHYT_RS17070: BPHYT_RS17070 - pyrimidine deaminase, at 3,870,447 to 3,870,770
_RS17070
BPHYT_RS17075: BPHYT_RS17075 - ABC transporter permease, at 3,870,780 to 3,871,709
_RS17075
Position (kb)
3869
3870
3871 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3869.473 kb on - strand at 3869.473 kb on - strand at 3869.491 kb on - strand at 3869.647 kb on - strand at 3869.680 kb on - strand at 3869.680 kb on - strand at 3869.682 kb on - strand at 3869.682 kb on - strand at 3869.711 kb on - strand at 3869.711 kb on - strand at 3870.422 kb on + strand at 3870.760 kb on - strand at 3870.787 kb on - strand at 3870.833 kb on - strand at 3870.833 kb on - strand at 3871.020 kb on + strand, within BPHYT_RS17075 at 3871.020 kb on + strand, within BPHYT_RS17075 at 3871.021 kb on - strand, within BPHYT_RS17075 at 3871.403 kb on - strand, within BPHYT_RS17075 at 3871.403 kb on - strand, within BPHYT_RS17075
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,869,473 - +0.1 3,869,473 - +0.2 3,869,491 - -0.8 3,869,647 - -1.6 3,869,680 - -0.9 3,869,680 - -0.9 3,869,682 - +0.2 3,869,682 - -0.2 3,869,711 - +1.3 3,869,711 - -0.9 3,870,422 + -2.9 3,870,760 - -1.3 3,870,787 - -0.4 3,870,833 - +0.6 3,870,833 - +0.9 3,871,020 + BPHYT_RS17075 0.26 -3.1 3,871,020 + BPHYT_RS17075 0.26 -3.0 3,871,021 - BPHYT_RS17075 0.26 -0.7 3,871,403 - BPHYT_RS17075 0.67 -0.2 3,871,403 - BPHYT_RS17075 0.67 -1.4
Or see this region's nucleotide sequence