Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS17050

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS17040 and BPHYT_RS17045 are separated by 248 nucleotidesBPHYT_RS17045 and BPHYT_RS17050 are separated by 30 nucleotidesBPHYT_RS17050 and BPHYT_RS17055 are separated by 86 nucleotides BPHYT_RS17040: BPHYT_RS17040 - membrane protein, at 3,862,577 to 3,863,053 _RS17040 BPHYT_RS17045: BPHYT_RS17045 - histone, at 3,863,302 to 3,863,919 _RS17045 BPHYT_RS17050: BPHYT_RS17050 - hypothetical protein, at 3,863,950 to 3,864,276 _RS17050 BPHYT_RS17055: BPHYT_RS17055 - ribonucleotide-diphosphate reductase subunit beta, at 3,864,363 to 3,865,598 _RS17055 Position (kb) 3863 3864 3865Strain fitness (log2 ratio) -1 0 1 2at 3863.230 kb on + strandat 3863.230 kb on + strandat 3863.278 kb on + strandat 3863.279 kb on - strandat 3863.686 kb on - strand, within BPHYT_RS17045at 3864.037 kb on - strand, within BPHYT_RS17050at 3864.056 kb on - strand, within BPHYT_RS17050

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,863,230 + +0.8
3,863,230 + +0.9
3,863,278 + -0.2
3,863,279 - +0.7
3,863,686 - BPHYT_RS17045 0.62 -0.8
3,864,037 - BPHYT_RS17050 0.27 +1.7
3,864,056 - BPHYT_RS17050 0.32 +2.1

Or see this region's nucleotide sequence