Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16970 and BPHYT_RS16975 are separated by 48 nucleotides BPHYT_RS16975 and BPHYT_RS16980 overlap by 4 nucleotides BPHYT_RS16980 and BPHYT_RS16985 are separated by 206 nucleotides BPHYT_RS16985 and BPHYT_RS16990 overlap by 4 nucleotides
BPHYT_RS16970: BPHYT_RS16970 - monofunctional biosynthetic peptidoglycan transglycosylase, at 3,848,460 to 3,849,182
_RS16970
BPHYT_RS16975: BPHYT_RS16975 - shikimate 5-dehydrogenase, at 3,849,231 to 3,850,094
_RS16975
BPHYT_RS16980: BPHYT_RS16980 - ribonuclease II, at 3,850,091 to 3,852,208
_RS16980
BPHYT_RS16985: BPHYT_RS16985 - esterase, at 3,852,415 to 3,853,017
_RS16985
BPHYT_RS16990: BPHYT_RS16990 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 3,853,014 to 3,854,408
_RS16990
Position (kb)
3850
3851
3852
3853 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3849.130 kb on + strand at 3849.220 kb on + strand at 3849.220 kb on + strand at 3849.221 kb on - strand at 3849.427 kb on + strand, within BPHYT_RS16975 at 3849.428 kb on - strand, within BPHYT_RS16975 at 3849.428 kb on - strand, within BPHYT_RS16975 at 3849.645 kb on - strand, within BPHYT_RS16975 at 3849.795 kb on - strand, within BPHYT_RS16975 at 3849.818 kb on + strand, within BPHYT_RS16975 at 3849.818 kb on + strand, within BPHYT_RS16975 at 3849.818 kb on + strand, within BPHYT_RS16975 at 3849.819 kb on - strand, within BPHYT_RS16975 at 3849.819 kb on - strand, within BPHYT_RS16975 at 3851.674 kb on - strand, within BPHYT_RS16980 at 3852.214 kb on - strand at 3852.214 kb on - strand at 3852.214 kb on - strand at 3852.301 kb on + strand at 3852.301 kb on + strand at 3852.396 kb on - strand at 3852.485 kb on + strand, within BPHYT_RS16985 at 3852.485 kb on + strand, within BPHYT_RS16985 at 3852.485 kb on + strand, within BPHYT_RS16985 at 3852.531 kb on + strand, within BPHYT_RS16985 at 3852.532 kb on - strand, within BPHYT_RS16985 at 3852.532 kb on - strand, within BPHYT_RS16985 at 3852.605 kb on + strand, within BPHYT_RS16985 at 3852.608 kb on + strand, within BPHYT_RS16985 at 3852.806 kb on + strand, within BPHYT_RS16985 at 3852.807 kb on - strand, within BPHYT_RS16985 at 3853.008 kb on - strand at 3853.008 kb on - strand at 3853.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,849,130 + -0.8 3,849,220 + -0.2 3,849,220 + -0.4 3,849,221 - +0.5 3,849,427 + BPHYT_RS16975 0.23 -1.9 3,849,428 - BPHYT_RS16975 0.23 -1.8 3,849,428 - BPHYT_RS16975 0.23 -2.0 3,849,645 - BPHYT_RS16975 0.48 -2.4 3,849,795 - BPHYT_RS16975 0.65 -0.4 3,849,818 + BPHYT_RS16975 0.68 -3.8 3,849,818 + BPHYT_RS16975 0.68 -0.5 3,849,818 + BPHYT_RS16975 0.68 -0.2 3,849,819 - BPHYT_RS16975 0.68 -1.6 3,849,819 - BPHYT_RS16975 0.68 -1.8 3,851,674 - BPHYT_RS16980 0.75 +0.8 3,852,214 - -1.8 3,852,214 - +1.8 3,852,214 - -0.3 3,852,301 + -1.2 3,852,301 + -0.1 3,852,396 - +0.2 3,852,485 + BPHYT_RS16985 0.12 -0.9 3,852,485 + BPHYT_RS16985 0.12 -0.8 3,852,485 + BPHYT_RS16985 0.12 -1.2 3,852,531 + BPHYT_RS16985 0.19 -0.6 3,852,532 - BPHYT_RS16985 0.19 +0.3 3,852,532 - BPHYT_RS16985 0.19 -0.3 3,852,605 + BPHYT_RS16985 0.32 -0.2 3,852,608 + BPHYT_RS16985 0.32 +0.1 3,852,806 + BPHYT_RS16985 0.65 -0.3 3,852,807 - BPHYT_RS16985 0.65 -0.6 3,853,008 - -0.3 3,853,008 - -1.8 3,853,008 - -0.4
Or see this region's nucleotide sequence