Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16755 and BPHYT_RS16760 are separated by 419 nucleotides BPHYT_RS16760 and BPHYT_RS16765 are separated by 404 nucleotides BPHYT_RS16765 and BPHYT_RS16770 are separated by 15 nucleotides
BPHYT_RS16755: BPHYT_RS16755 - hypothetical protein, at 3,803,676 to 3,804,116
_RS16755
BPHYT_RS16760: BPHYT_RS16760 - hypothetical protein, at 3,804,536 to 3,804,973
_RS16760
BPHYT_RS16765: BPHYT_RS16765 - membrane protein, at 3,805,378 to 3,805,794
_RS16765
BPHYT_RS16770: BPHYT_RS16770 - gamma-glutamyl phosphate reductase, at 3,805,810 to 3,807,081
_RS16770
Position (kb)
3804
3805 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3803.553 kb on - strand at 3803.648 kb on + strand at 3803.648 kb on + strand at 3803.844 kb on + strand, within BPHYT_RS16755 at 3803.844 kb on + strand, within BPHYT_RS16755 at 3803.844 kb on + strand, within BPHYT_RS16755 at 3803.844 kb on + strand, within BPHYT_RS16755 at 3803.844 kb on + strand, within BPHYT_RS16755 at 3803.845 kb on - strand, within BPHYT_RS16755 at 3803.845 kb on - strand, within BPHYT_RS16755 at 3803.963 kb on + strand, within BPHYT_RS16755 at 3804.024 kb on + strand, within BPHYT_RS16755 at 3804.115 kb on - strand at 3804.128 kb on + strand at 3804.128 kb on + strand at 3804.164 kb on - strand at 3804.439 kb on - strand at 3804.439 kb on - strand at 3804.439 kb on - strand at 3804.522 kb on + strand at 3804.526 kb on + strand at 3804.526 kb on + strand at 3804.527 kb on - strand at 3804.527 kb on - strand at 3804.828 kb on + strand, within BPHYT_RS16760 at 3804.829 kb on - strand, within BPHYT_RS16760 at 3804.830 kb on + strand, within BPHYT_RS16760 at 3805.135 kb on - strand at 3805.223 kb on + strand at 3805.257 kb on - strand at 3805.258 kb on + strand at 3805.258 kb on + strand at 3805.259 kb on - strand at 3805.259 kb on - strand at 3805.275 kb on + strand at 3805.321 kb on - strand at 3805.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,803,553 - +3.7 3,803,648 + +0.9 3,803,648 + -1.4 3,803,844 + BPHYT_RS16755 0.38 -0.1 3,803,844 + BPHYT_RS16755 0.38 -0.3 3,803,844 + BPHYT_RS16755 0.38 -2.1 3,803,844 + BPHYT_RS16755 0.38 +1.5 3,803,844 + BPHYT_RS16755 0.38 -2.4 3,803,845 - BPHYT_RS16755 0.38 +0.2 3,803,845 - BPHYT_RS16755 0.38 -0.2 3,803,963 + BPHYT_RS16755 0.65 -1.6 3,804,024 + BPHYT_RS16755 0.79 -0.8 3,804,115 - -1.1 3,804,128 + +0.1 3,804,128 + +0.3 3,804,164 - +0.8 3,804,439 - +0.1 3,804,439 - +0.0 3,804,439 - -0.1 3,804,522 + +0.6 3,804,526 + +0.3 3,804,526 + -0.1 3,804,527 - -1.6 3,804,527 - -1.0 3,804,828 + BPHYT_RS16760 0.67 -0.2 3,804,829 - BPHYT_RS16760 0.67 -1.6 3,804,830 + BPHYT_RS16760 0.67 +4.5 3,805,135 - +1.6 3,805,223 + +1.2 3,805,257 - +0.1 3,805,258 + +0.1 3,805,258 + -2.4 3,805,259 - -0.3 3,805,259 - +0.8 3,805,275 + +0.4 3,805,321 - +0.2 3,805,361 - +0.4
Or see this region's nucleotide sequence