Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16725 and BPHYT_RS16730 are separated by 259 nucleotides BPHYT_RS16730 and BPHYT_RS16735 are separated by 25 nucleotides BPHYT_RS16735 and BPHYT_RS16740 are separated by 296 nucleotides
BPHYT_RS16725: BPHYT_RS16725 - gluconate:H+ symporter (gntT) (from data) , at 3,786,056 to 3,787,453
_RS16725
BPHYT_RS16730: BPHYT_RS16730 - 2-dehydro-3-deoxyphosphogluconate aldolase, at 3,787,713 to 3,788,354
_RS16730
BPHYT_RS16735: BPHYT_RS16735 - phosphogluconate dehydratase, at 3,788,380 to 3,790,284
_RS16735
BPHYT_RS16740: BPHYT_RS16740 - transcriptional regulator, at 3,790,581 to 3,791,426
_RS16740
Position (kb)
3788
3789
3790
3791 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 3787.581 kb on + strand at 3787.582 kb on - strand at 3787.652 kb on - strand at 3787.714 kb on + strand at 3787.715 kb on - strand at 3787.715 kb on - strand at 3788.355 kb on - strand at 3788.355 kb on - strand at 3788.382 kb on - strand at 3788.515 kb on + strand at 3788.515 kb on + strand at 3789.135 kb on - strand, within BPHYT_RS16735 at 3789.267 kb on - strand, within BPHYT_RS16735 at 3789.452 kb on + strand, within BPHYT_RS16735 at 3789.466 kb on + strand, within BPHYT_RS16735 at 3789.466 kb on + strand, within BPHYT_RS16735 at 3789.467 kb on - strand, within BPHYT_RS16735 at 3789.525 kb on + strand, within BPHYT_RS16735 at 3789.605 kb on + strand, within BPHYT_RS16735 at 3789.605 kb on + strand, within BPHYT_RS16735 at 3789.633 kb on - strand, within BPHYT_RS16735 at 3789.633 kb on - strand, within BPHYT_RS16735 at 3790.056 kb on - strand, within BPHYT_RS16735 at 3790.208 kb on + strand at 3790.209 kb on - strand at 3790.209 kb on - strand at 3790.541 kb on - strand at 3790.542 kb on + strand at 3790.543 kb on - strand at 3790.544 kb on + strand at 3790.565 kb on + strand at 3790.566 kb on - strand at 3790.647 kb on + strand at 3790.647 kb on + strand at 3790.647 kb on + strand at 3790.648 kb on - strand at 3790.780 kb on + strand, within BPHYT_RS16740 at 3790.780 kb on + strand, within BPHYT_RS16740 at 3790.822 kb on - strand, within BPHYT_RS16740 at 3790.951 kb on + strand, within BPHYT_RS16740 at 3790.977 kb on + strand, within BPHYT_RS16740 at 3790.977 kb on + strand, within BPHYT_RS16740 at 3790.978 kb on - strand, within BPHYT_RS16740 at 3791.069 kb on + strand, within BPHYT_RS16740 at 3791.069 kb on + strand, within BPHYT_RS16740 at 3791.073 kb on + strand, within BPHYT_RS16740 at 3791.074 kb on - strand, within BPHYT_RS16740 at 3791.074 kb on - strand, within BPHYT_RS16740
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,787,581 + -0.3 3,787,582 - +0.1 3,787,652 - +0.5 3,787,714 + -0.8 3,787,715 - -1.0 3,787,715 - +0.2 3,788,355 - -0.5 3,788,355 - +3.3 3,788,382 - +0.4 3,788,515 + -1.9 3,788,515 + +0.8 3,789,135 - BPHYT_RS16735 0.40 +1.2 3,789,267 - BPHYT_RS16735 0.47 -0.3 3,789,452 + BPHYT_RS16735 0.56 +2.0 3,789,466 + BPHYT_RS16735 0.57 +1.2 3,789,466 + BPHYT_RS16735 0.57 +1.2 3,789,467 - BPHYT_RS16735 0.57 +0.9 3,789,525 + BPHYT_RS16735 0.60 +1.4 3,789,605 + BPHYT_RS16735 0.64 +0.2 3,789,605 + BPHYT_RS16735 0.64 +7.2 3,789,633 - BPHYT_RS16735 0.66 +0.5 3,789,633 - BPHYT_RS16735 0.66 +0.6 3,790,056 - BPHYT_RS16735 0.88 -0.3 3,790,208 + +1.6 3,790,209 - -0.2 3,790,209 - +0.3 3,790,541 - +1.9 3,790,542 + -0.2 3,790,543 - +1.3 3,790,544 + +0.1 3,790,565 + -1.0 3,790,566 - -0.5 3,790,647 + -3.9 3,790,647 + -0.8 3,790,647 + -3.3 3,790,648 - -0.5 3,790,780 + BPHYT_RS16740 0.24 +1.2 3,790,780 + BPHYT_RS16740 0.24 -0.7 3,790,822 - BPHYT_RS16740 0.28 -0.8 3,790,951 + BPHYT_RS16740 0.44 -1.7 3,790,977 + BPHYT_RS16740 0.47 -1.4 3,790,977 + BPHYT_RS16740 0.47 +0.5 3,790,978 - BPHYT_RS16740 0.47 -2.0 3,791,069 + BPHYT_RS16740 0.58 -1.5 3,791,069 + BPHYT_RS16740 0.58 -0.5 3,791,073 + BPHYT_RS16740 0.58 -1.8 3,791,074 - BPHYT_RS16740 0.58 +3.9 3,791,074 - BPHYT_RS16740 0.58 -1.8
Or see this region's nucleotide sequence