Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16325 and BPHYT_RS16330 are separated by 158 nucleotides BPHYT_RS16330 and BPHYT_RS16335 are separated by 143 nucleotides BPHYT_RS16335 and BPHYT_RS16340 are separated by 144 nucleotides
BPHYT_RS16325: BPHYT_RS16325 - polymerase, at 3,704,474 to 3,706,258
_RS16325
BPHYT_RS16330: BPHYT_RS16330 - general secretion pathway protein H, at 3,706,417 to 3,707,040
_RS16330
BPHYT_RS16335: BPHYT_RS16335 - membrane protein, at 3,707,184 to 3,707,888
_RS16335
BPHYT_RS16340: BPHYT_RS16340 - succinyl-CoA synthetase subunit alpha, at 3,708,033 to 3,708,914
_RS16340
Position (kb)
3706
3707
3708 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3706.294 kb on + strand at 3706.354 kb on - strand at 3706.842 kb on - strand, within BPHYT_RS16330 at 3706.865 kb on + strand, within BPHYT_RS16330 at 3707.012 kb on + strand at 3707.022 kb on - strand at 3707.022 kb on - strand at 3707.091 kb on + strand at 3707.091 kb on + strand at 3707.092 kb on - strand at 3707.092 kb on - strand at 3707.092 kb on - strand at 3707.153 kb on - strand at 3707.153 kb on - strand at 3707.286 kb on - strand, within BPHYT_RS16335 at 3707.900 kb on + strand at 3707.901 kb on - strand at 3707.901 kb on - strand at 3707.944 kb on + strand at 3707.945 kb on - strand at 3707.945 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,706,294 + -1.7 3,706,354 - +0.5 3,706,842 - BPHYT_RS16330 0.68 -0.2 3,706,865 + BPHYT_RS16330 0.72 +0.1 3,707,012 + -0.2 3,707,022 - -0.9 3,707,022 - +0.5 3,707,091 + -0.7 3,707,091 + -0.2 3,707,092 - -0.0 3,707,092 - +0.0 3,707,092 - +2.3 3,707,153 - +1.9 3,707,153 - +0.5 3,707,286 - BPHYT_RS16335 0.14 -0.9 3,707,900 + -0.2 3,707,901 - -0.7 3,707,901 - +0.0 3,707,944 + -0.4 3,707,945 - -1.8 3,707,945 - +1.0
Or see this region's nucleotide sequence