Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS16325

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16315 and BPHYT_RS16320 are separated by 244 nucleotidesBPHYT_RS16320 and BPHYT_RS16325 are separated by 98 nucleotidesBPHYT_RS16325 and BPHYT_RS16330 are separated by 158 nucleotidesBPHYT_RS16330 and BPHYT_RS16335 are separated by 143 nucleotides BPHYT_RS16315: BPHYT_RS16315 - peptidase M48 Ste24p, at 3,701,945 to 3,703,654 _RS16315 BPHYT_RS16320: BPHYT_RS16320 - cyclic pyranopterin monophosphate synthase accessory protein, at 3,703,899 to 3,704,375 _RS16320 BPHYT_RS16325: BPHYT_RS16325 - polymerase, at 3,704,474 to 3,706,258 _RS16325 BPHYT_RS16330: BPHYT_RS16330 - general secretion pathway protein H, at 3,706,417 to 3,707,040 _RS16330 BPHYT_RS16335: BPHYT_RS16335 - membrane protein, at 3,707,184 to 3,707,888 _RS16335 Position (kb) 3704 3705 3706 3707Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3703.564 kb on + strandat 3703.703 kb on - strandat 3703.703 kb on - strandat 3703.727 kb on + strandat 3703.727 kb on + strandat 3703.868 kb on + strandat 3703.868 kb on + strandat 3703.868 kb on + strandat 3703.868 kb on + strandat 3703.869 kb on - strandat 3703.873 kb on + strandat 3703.874 kb on - strandat 3703.885 kb on + strandat 3703.940 kb on + strandat 3703.940 kb on + strandat 3703.940 kb on + strandat 3704.277 kb on + strand, within BPHYT_RS16320at 3704.278 kb on - strand, within BPHYT_RS16320at 3704.278 kb on - strand, within BPHYT_RS16320at 3704.278 kb on - strand, within BPHYT_RS16320at 3704.408 kb on + strandat 3704.467 kb on + strandat 3704.468 kb on - strandat 3704.593 kb on - strandat 3706.294 kb on + strandat 3706.354 kb on - strandat 3706.842 kb on - strand, within BPHYT_RS16330at 3706.865 kb on + strand, within BPHYT_RS16330at 3707.012 kb on + strandat 3707.022 kb on - strandat 3707.022 kb on - strandat 3707.091 kb on + strandat 3707.091 kb on + strandat 3707.092 kb on - strandat 3707.092 kb on - strandat 3707.092 kb on - strandat 3707.153 kb on - strandat 3707.153 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,703,564 + +1.0
3,703,703 - +1.0
3,703,703 - -0.4
3,703,727 + +0.5
3,703,727 + -0.5
3,703,868 + +0.5
3,703,868 + -0.2
3,703,868 + +0.5
3,703,868 + +0.7
3,703,869 - +1.1
3,703,873 + -1.3
3,703,874 - +0.3
3,703,885 + +2.4
3,703,940 + -2.8
3,703,940 + -1.2
3,703,940 + -3.4
3,704,277 + BPHYT_RS16320 0.79 -0.7
3,704,278 - BPHYT_RS16320 0.79 -3.1
3,704,278 - BPHYT_RS16320 0.79 -1.8
3,704,278 - BPHYT_RS16320 0.79 -3.8
3,704,408 + +0.5
3,704,467 + +1.5
3,704,468 - +0.6
3,704,593 - -0.5
3,706,294 + -1.7
3,706,354 - +0.5
3,706,842 - BPHYT_RS16330 0.68 -0.2
3,706,865 + BPHYT_RS16330 0.72 +0.1
3,707,012 + -0.2
3,707,022 - -0.9
3,707,022 - +0.5
3,707,091 + -0.7
3,707,091 + -0.2
3,707,092 - -0.0
3,707,092 - +0.0
3,707,092 - +2.3
3,707,153 - +1.9
3,707,153 - +0.5

Or see this region's nucleotide sequence