Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15480 and BPHYT_RS15485 are separated by 114 nucleotides BPHYT_RS15485 and BPHYT_RS15490 are separated by 252 nucleotides BPHYT_RS15490 and BPHYT_RS15495 are separated by 106 nucleotides BPHYT_RS15495 and BPHYT_RS15500 are separated by 150 nucleotides
BPHYT_RS15480: BPHYT_RS15480 - hypothetical protein, at 3,512,864 to 3,513,457
_RS15480
BPHYT_RS15485: BPHYT_RS15485 - proteinase inhibitor, at 3,513,572 to 3,513,874
_RS15485
BPHYT_RS15490: BPHYT_RS15490 - coproporphyrinogen III oxidase, at 3,514,127 to 3,515,512
_RS15490
BPHYT_RS15495: BPHYT_RS15495 - tRNA-Leu, at 3,515,619 to 3,515,703
_RS15495
BPHYT_RS15500: BPHYT_RS15500 - aromatic amino acid transporter, at 3,515,854 to 3,517,239
_RS15500
Position (kb)
3514
3515
3516 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 3513.277 kb on - strand, within BPHYT_RS15480 at 3513.397 kb on + strand, within BPHYT_RS15480 at 3513.492 kb on + strand at 3513.493 kb on - strand at 3513.567 kb on + strand at 3513.567 kb on + strand at 3513.567 kb on + strand at 3513.812 kb on - strand, within BPHYT_RS15485 at 3513.938 kb on + strand at 3513.980 kb on + strand at 3514.173 kb on - strand at 3514.195 kb on + strand at 3514.195 kb on + strand at 3514.195 kb on + strand at 3514.195 kb on + strand at 3514.196 kb on - strand at 3514.196 kb on - strand at 3514.196 kb on - strand at 3514.251 kb on + strand at 3514.299 kb on - strand, within BPHYT_RS15490 at 3514.565 kb on + strand, within BPHYT_RS15490 at 3514.775 kb on + strand, within BPHYT_RS15490 at 3514.775 kb on + strand, within BPHYT_RS15490 at 3514.865 kb on + strand, within BPHYT_RS15490 at 3514.986 kb on - strand, within BPHYT_RS15490 at 3514.986 kb on - strand, within BPHYT_RS15490 at 3514.991 kb on + strand, within BPHYT_RS15490 at 3514.991 kb on + strand, within BPHYT_RS15490 at 3515.315 kb on + strand, within BPHYT_RS15490 at 3515.316 kb on - strand, within BPHYT_RS15490 at 3515.855 kb on + strand at 3515.912 kb on + strand at 3515.912 kb on + strand at 3515.913 kb on - strand at 3515.913 kb on - strand at 3516.188 kb on + strand, within BPHYT_RS15500 at 3516.189 kb on - strand, within BPHYT_RS15500 at 3516.240 kb on + strand, within BPHYT_RS15500 at 3516.241 kb on - strand, within BPHYT_RS15500 at 3516.242 kb on + strand, within BPHYT_RS15500 at 3516.242 kb on + strand, within BPHYT_RS15500 at 3516.242 kb on + strand, within BPHYT_RS15500 at 3516.242 kb on + strand, within BPHYT_RS15500 at 3516.243 kb on - strand, within BPHYT_RS15500 at 3516.243 kb on - strand, within BPHYT_RS15500 at 3516.243 kb on - strand, within BPHYT_RS15500 at 3516.308 kb on + strand, within BPHYT_RS15500 at 3516.329 kb on + strand, within BPHYT_RS15500 at 3516.379 kb on - strand, within BPHYT_RS15500 at 3516.498 kb on - strand, within BPHYT_RS15500
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,513,277 - BPHYT_RS15480 0.70 +0.2 3,513,397 + BPHYT_RS15480 0.90 -0.2 3,513,492 + +0.1 3,513,493 - -1.0 3,513,567 + -0.2 3,513,567 + -0.2 3,513,567 + +0.9 3,513,812 - BPHYT_RS15485 0.79 -0.6 3,513,938 + +0.9 3,513,980 + -0.1 3,514,173 - +2.5 3,514,195 + +1.7 3,514,195 + -0.3 3,514,195 + +1.6 3,514,195 + -1.3 3,514,196 - +0.3 3,514,196 - -0.5 3,514,196 - +0.3 3,514,251 + +2.1 3,514,299 - BPHYT_RS15490 0.12 +1.0 3,514,565 + BPHYT_RS15490 0.32 +2.0 3,514,775 + BPHYT_RS15490 0.47 +1.3 3,514,775 + BPHYT_RS15490 0.47 +2.3 3,514,865 + BPHYT_RS15490 0.53 +1.8 3,514,986 - BPHYT_RS15490 0.62 +6.0 3,514,986 - BPHYT_RS15490 0.62 -1.0 3,514,991 + BPHYT_RS15490 0.62 +0.3 3,514,991 + BPHYT_RS15490 0.62 +0.5 3,515,315 + BPHYT_RS15490 0.86 -1.0 3,515,316 - BPHYT_RS15490 0.86 +1.1 3,515,855 + +0.0 3,515,912 + +0.6 3,515,912 + -0.2 3,515,913 - +0.3 3,515,913 - -0.5 3,516,188 + BPHYT_RS15500 0.24 -1.6 3,516,189 - BPHYT_RS15500 0.24 -0.4 3,516,240 + BPHYT_RS15500 0.28 -0.1 3,516,241 - BPHYT_RS15500 0.28 +1.5 3,516,242 + BPHYT_RS15500 0.28 +0.5 3,516,242 + BPHYT_RS15500 0.28 -0.5 3,516,242 + BPHYT_RS15500 0.28 -1.3 3,516,242 + BPHYT_RS15500 0.28 +1.2 3,516,243 - BPHYT_RS15500 0.28 +0.1 3,516,243 - BPHYT_RS15500 0.28 -0.6 3,516,243 - BPHYT_RS15500 0.28 -0.4 3,516,308 + BPHYT_RS15500 0.33 +0.1 3,516,329 + BPHYT_RS15500 0.34 -0.3 3,516,379 - BPHYT_RS15500 0.38 +0.8 3,516,498 - BPHYT_RS15500 0.46 -1.0
Or see this region's nucleotide sequence