Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15280 and BPHYT_RS15285 are separated by 118 nucleotides BPHYT_RS15285 and BPHYT_RS15290 are separated by 120 nucleotides BPHYT_RS15290 and BPHYT_RS15295 are separated by 79 nucleotides BPHYT_RS15295 and BPHYT_RS15300 are separated by 113 nucleotides
BPHYT_RS15280: BPHYT_RS15280 - porin, at 3,473,643 to 3,474,788
_RS15280
BPHYT_RS15285: BPHYT_RS15285 - transcription elongation factor GreB, at 3,474,907 to 3,475,476
_RS15285
BPHYT_RS15290: BPHYT_RS15290 - tRNA-Arg, at 3,475,597 to 3,475,673
_RS15290
BPHYT_RS15295: BPHYT_RS15295 - tRNA-Arg, at 3,475,753 to 3,475,829
_RS15295
BPHYT_RS15300: BPHYT_RS15300 - hypothetical protein, at 3,475,943 to 3,476,452
_RS15300
Position (kb) 3474
3475
3476 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3474.132 kb on + strand, within BPHYT_RS15280 at 3474.132 kb on + strand, within BPHYT_RS15280 at 3474.159 kb on + strand, within BPHYT_RS15280 at 3474.379 kb on - strand, within BPHYT_RS15280 at 3474.411 kb on + strand, within BPHYT_RS15280 at 3474.567 kb on + strand, within BPHYT_RS15280 at 3474.567 kb on + strand, within BPHYT_RS15280 at 3474.573 kb on + strand, within BPHYT_RS15280 at 3474.574 kb on - strand, within BPHYT_RS15280 at 3474.663 kb on + strand, within BPHYT_RS15280 at 3474.663 kb on + strand, within BPHYT_RS15280 at 3474.664 kb on - strand, within BPHYT_RS15280 at 3474.844 kb on + strand at 3474.845 kb on - strand at 3474.932 kb on + strand at 3474.947 kb on - strand at 3474.948 kb on + strand at 3474.977 kb on + strand, within BPHYT_RS15285 at 3474.977 kb on + strand, within BPHYT_RS15285 at 3474.978 kb on - strand, within BPHYT_RS15285 at 3475.093 kb on - strand, within BPHYT_RS15285 at 3475.251 kb on - strand, within BPHYT_RS15285 at 3475.257 kb on - strand, within BPHYT_RS15285 at 3475.385 kb on + strand, within BPHYT_RS15285 at 3475.508 kb on + strand at 3475.508 kb on + strand at 3475.509 kb on - strand at 3475.517 kb on + strand at 3475.518 kb on - strand at 3475.531 kb on + strand at 3475.532 kb on - strand at 3475.604 kb on + strand at 3475.622 kb on + strand, within BPHYT_RS15290 at 3475.622 kb on + strand, within BPHYT_RS15290 at 3475.622 kb on + strand, within BPHYT_RS15290 at 3475.676 kb on + strand at 3475.676 kb on + strand at 3475.713 kb on + strand at 3475.733 kb on - strand at 3475.747 kb on + strand at 3475.832 kb on + strand at 3476.302 kb on + strand, within BPHYT_RS15300 at 3476.303 kb on - strand, within BPHYT_RS15300 at 3476.373 kb on + strand, within BPHYT_RS15300
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,474,132 + BPHYT_RS15280 0.43 +0.3 3,474,132 + BPHYT_RS15280 0.43 -0.6 3,474,159 + BPHYT_RS15280 0.45 -1.9 3,474,379 - BPHYT_RS15280 0.64 -1.9 3,474,411 + BPHYT_RS15280 0.67 -0.3 3,474,567 + BPHYT_RS15280 0.81 -0.7 3,474,567 + BPHYT_RS15280 0.81 -0.4 3,474,573 + BPHYT_RS15280 0.81 -0.9 3,474,574 - BPHYT_RS15280 0.81 -1.0 3,474,663 + BPHYT_RS15280 0.89 -0.7 3,474,663 + BPHYT_RS15280 0.89 -1.4 3,474,664 - BPHYT_RS15280 0.89 -0.0 3,474,844 + +1.6 3,474,845 - -0.5 3,474,932 + +0.7 3,474,947 - +1.2 3,474,948 + +1.8 3,474,977 + BPHYT_RS15285 0.12 +1.3 3,474,977 + BPHYT_RS15285 0.12 +1.6 3,474,978 - BPHYT_RS15285 0.12 +1.6 3,475,093 - BPHYT_RS15285 0.33 -0.2 3,475,251 - BPHYT_RS15285 0.60 +1.6 3,475,257 - BPHYT_RS15285 0.61 -0.1 3,475,385 + BPHYT_RS15285 0.84 -0.2 3,475,508 + +0.3 3,475,508 + -0.3 3,475,509 - -0.9 3,475,517 + -0.9 3,475,518 - -1.2 3,475,531 + +0.3 3,475,532 - -0.6 3,475,604 + -0.8 3,475,622 + BPHYT_RS15290 0.32 -1.7 3,475,622 + BPHYT_RS15290 0.32 -0.5 3,475,622 + BPHYT_RS15290 0.32 -2.2 3,475,676 + -1.0 3,475,676 + -1.1 3,475,713 + +0.2 3,475,733 - +1.3 3,475,747 + -0.1 3,475,832 + -0.1 3,476,302 + BPHYT_RS15300 0.70 -0.9 3,476,303 - BPHYT_RS15300 0.71 +0.3 3,476,373 + BPHYT_RS15300 0.84 +0.1
Or see this region's nucleotide sequence