Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15225 and BPHYT_RS15230 are separated by 55 nucleotides BPHYT_RS15230 and BPHYT_RS15235 are separated by 101 nucleotides BPHYT_RS15235 and BPHYT_RS15240 are separated by 262 nucleotides
BPHYT_RS15225: BPHYT_RS15225 - fructose-1,6-bisphosphatase class 1, at 3,463,358 to 3,464,374
_RS15225
BPHYT_RS15230: BPHYT_RS15230 - tRNA-Thr, at 3,464,430 to 3,464,505
_RS15230
BPHYT_RS15235: BPHYT_RS15235 - hypothetical protein, at 3,464,607 to 3,464,813
_RS15235
BPHYT_RS15240: BPHYT_RS15240 - excinuclease ABC subunit A, at 3,465,076 to 3,467,760
_RS15240
Position (kb)
3464
3465 Strain fitness (log2 ratio)
-1
0
1
2 at 3464.584 kb on + strand at 3464.585 kb on - strand at 3464.711 kb on + strand, within BPHYT_RS15235 at 3464.712 kb on - strand, within BPHYT_RS15235 at 3464.712 kb on - strand, within BPHYT_RS15235 at 3464.713 kb on + strand, within BPHYT_RS15235 at 3464.912 kb on + strand at 3464.939 kb on - strand at 3465.058 kb on + strand at 3465.268 kb on + strand at 3465.268 kb on + strand at 3465.456 kb on - strand, within BPHYT_RS15240 at 3465.467 kb on - strand, within BPHYT_RS15240 at 3465.578 kb on - strand, within BPHYT_RS15240 at 3465.589 kb on + strand, within BPHYT_RS15240 at 3465.700 kb on + strand, within BPHYT_RS15240 at 3465.700 kb on + strand, within BPHYT_RS15240 at 3465.788 kb on - strand, within BPHYT_RS15240
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,464,584 + +2.2 3,464,585 - +0.9 3,464,711 + BPHYT_RS15235 0.50 +0.5 3,464,712 - BPHYT_RS15235 0.51 -1.3 3,464,712 - BPHYT_RS15235 0.51 -0.9 3,464,713 + BPHYT_RS15235 0.51 -0.1 3,464,912 + +0.7 3,464,939 - +0.6 3,465,058 + +0.9 3,465,268 + -1.4 3,465,268 + +1.2 3,465,456 - BPHYT_RS15240 0.14 -0.6 3,465,467 - BPHYT_RS15240 0.15 -0.4 3,465,578 - BPHYT_RS15240 0.19 -0.6 3,465,589 + BPHYT_RS15240 0.19 +0.3 3,465,700 + BPHYT_RS15240 0.23 +0.6 3,465,700 + BPHYT_RS15240 0.23 +0.5 3,465,788 - BPHYT_RS15240 0.27 -0.1
Or see this region's nucleotide sequence