Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15125

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15115 and BPHYT_RS15120 are separated by 60 nucleotidesBPHYT_RS15120 and BPHYT_RS15125 are separated by 39 nucleotidesBPHYT_RS15125 and BPHYT_RS15130 overlap by 4 nucleotides BPHYT_RS15115: BPHYT_RS15115 - excinuclease ABC subunit A, at 3,440,843 to 3,441,286 _RS15115 BPHYT_RS15120: BPHYT_RS15120 - hypothetical protein, at 3,441,347 to 3,441,769 _RS15120 BPHYT_RS15125: BPHYT_RS15125 - 3-oxoacyl-ACP synthase, at 3,441,809 to 3,443,050 _RS15125 BPHYT_RS15130: BPHYT_RS15130 - 3-oxoacyl-ACP synthase, at 3,443,047 to 3,444,273 _RS15130 Position (kb) 3441 3442 3443 3444Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3440.844 kb on + strandat 3440.845 kb on - strandat 3440.845 kb on - strandat 3441.198 kb on + strand, within BPHYT_RS15115at 3441.199 kb on - strand, within BPHYT_RS15115at 3441.448 kb on + strand, within BPHYT_RS15120at 3441.642 kb on + strand, within BPHYT_RS15120at 3441.752 kb on + strandat 3441.794 kb on - strandat 3441.864 kb on + strandat 3441.999 kb on + strand, within BPHYT_RS15125at 3442.165 kb on - strand, within BPHYT_RS15125at 3442.165 kb on - strand, within BPHYT_RS15125at 3442.288 kb on + strand, within BPHYT_RS15125at 3442.288 kb on + strand, within BPHYT_RS15125at 3442.288 kb on + strand, within BPHYT_RS15125at 3442.381 kb on - strand, within BPHYT_RS15125at 3442.576 kb on - strand, within BPHYT_RS15125at 3442.650 kb on + strand, within BPHYT_RS15125at 3442.705 kb on - strand, within BPHYT_RS15125at 3442.760 kb on + strand, within BPHYT_RS15125at 3442.988 kb on - strandat 3443.076 kb on + strandat 3443.076 kb on + strandat 3443.077 kb on - strandat 3443.077 kb on - strandat 3443.077 kb on - strandat 3443.114 kb on + strandat 3443.114 kb on + strandat 3443.151 kb on - strandat 3443.178 kb on + strand, within BPHYT_RS15130at 3443.179 kb on - strand, within BPHYT_RS15130at 3443.204 kb on + strand, within BPHYT_RS15130at 3443.279 kb on + strand, within BPHYT_RS15130at 3443.279 kb on + strand, within BPHYT_RS15130at 3443.546 kb on + strand, within BPHYT_RS15130at 3443.547 kb on - strand, within BPHYT_RS15130at 3443.547 kb on - strand, within BPHYT_RS15130at 3443.657 kb on + strandat 3443.751 kb on - strand, within BPHYT_RS15130at 3443.870 kb on + strand, within BPHYT_RS15130at 3443.871 kb on - strand, within BPHYT_RS15130at 3443.871 kb on - strand, within BPHYT_RS15130at 3443.961 kb on - strand, within BPHYT_RS15130at 3444.047 kb on + strand, within BPHYT_RS15130at 3444.047 kb on + strand, within BPHYT_RS15130at 3444.048 kb on - strand, within BPHYT_RS15130

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,440,844 + +3.3
3,440,845 - -0.5
3,440,845 - +1.4
3,441,198 + BPHYT_RS15115 0.80 +0.1
3,441,199 - BPHYT_RS15115 0.80 +0.4
3,441,448 + BPHYT_RS15120 0.24 -0.5
3,441,642 + BPHYT_RS15120 0.70 +1.8
3,441,752 + +0.7
3,441,794 - -0.0
3,441,864 + +1.1
3,441,999 + BPHYT_RS15125 0.15 +0.1
3,442,165 - BPHYT_RS15125 0.29 +0.2
3,442,165 - BPHYT_RS15125 0.29 +0.4
3,442,288 + BPHYT_RS15125 0.39 +1.1
3,442,288 + BPHYT_RS15125 0.39 +0.7
3,442,288 + BPHYT_RS15125 0.39 +0.0
3,442,381 - BPHYT_RS15125 0.46 +0.5
3,442,576 - BPHYT_RS15125 0.62 -0.0
3,442,650 + BPHYT_RS15125 0.68 +1.0
3,442,705 - BPHYT_RS15125 0.72 +0.2
3,442,760 + BPHYT_RS15125 0.77 -1.2
3,442,988 - +0.6
3,443,076 + -1.6
3,443,076 + -1.1
3,443,077 - +0.1
3,443,077 - -1.6
3,443,077 - -1.0
3,443,114 + +0.0
3,443,114 + -1.3
3,443,151 - +0.2
3,443,178 + BPHYT_RS15130 0.11 +0.9
3,443,179 - BPHYT_RS15130 0.11 -1.7
3,443,204 + BPHYT_RS15130 0.13 +0.9
3,443,279 + BPHYT_RS15130 0.19 +0.2
3,443,279 + BPHYT_RS15130 0.19 +2.1
3,443,546 + BPHYT_RS15130 0.41 -1.5
3,443,547 - BPHYT_RS15130 0.41 -0.5
3,443,547 - BPHYT_RS15130 0.41 -0.8
3,443,657 + +2.2
3,443,751 - BPHYT_RS15130 0.57 +0.3
3,443,870 + BPHYT_RS15130 0.67 -0.6
3,443,871 - BPHYT_RS15130 0.67 -2.5
3,443,871 - BPHYT_RS15130 0.67 -0.4
3,443,961 - BPHYT_RS15130 0.74 +1.1
3,444,047 + BPHYT_RS15130 0.81 +0.3
3,444,047 + BPHYT_RS15130 0.81 -0.9
3,444,048 - BPHYT_RS15130 0.82 +0.3

Or see this region's nucleotide sequence