Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15115 and BPHYT_RS15120 are separated by 60 nucleotides BPHYT_RS15120 and BPHYT_RS15125 are separated by 39 nucleotides BPHYT_RS15125 and BPHYT_RS15130 overlap by 4 nucleotides
BPHYT_RS15115: BPHYT_RS15115 - excinuclease ABC subunit A, at 3,440,843 to 3,441,286
_RS15115
BPHYT_RS15120: BPHYT_RS15120 - hypothetical protein, at 3,441,347 to 3,441,769
_RS15120
BPHYT_RS15125: BPHYT_RS15125 - 3-oxoacyl-ACP synthase, at 3,441,809 to 3,443,050
_RS15125
BPHYT_RS15130: BPHYT_RS15130 - 3-oxoacyl-ACP synthase, at 3,443,047 to 3,444,273
_RS15130
Position (kb)
3441
3442
3443
3444 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3440.844 kb on + strand at 3440.845 kb on - strand at 3440.845 kb on - strand at 3441.198 kb on + strand, within BPHYT_RS15115 at 3441.199 kb on - strand, within BPHYT_RS15115 at 3441.448 kb on + strand, within BPHYT_RS15120 at 3441.642 kb on + strand, within BPHYT_RS15120 at 3441.752 kb on + strand at 3441.794 kb on - strand at 3441.864 kb on + strand at 3441.999 kb on + strand, within BPHYT_RS15125 at 3442.165 kb on - strand, within BPHYT_RS15125 at 3442.165 kb on - strand, within BPHYT_RS15125 at 3442.288 kb on + strand, within BPHYT_RS15125 at 3442.288 kb on + strand, within BPHYT_RS15125 at 3442.288 kb on + strand, within BPHYT_RS15125 at 3442.381 kb on - strand, within BPHYT_RS15125 at 3442.576 kb on - strand, within BPHYT_RS15125 at 3442.650 kb on + strand, within BPHYT_RS15125 at 3442.705 kb on - strand, within BPHYT_RS15125 at 3442.760 kb on + strand, within BPHYT_RS15125 at 3442.988 kb on - strand at 3443.076 kb on + strand at 3443.076 kb on + strand at 3443.077 kb on - strand at 3443.077 kb on - strand at 3443.077 kb on - strand at 3443.114 kb on + strand at 3443.114 kb on + strand at 3443.151 kb on - strand at 3443.178 kb on + strand, within BPHYT_RS15130 at 3443.179 kb on - strand, within BPHYT_RS15130 at 3443.204 kb on + strand, within BPHYT_RS15130 at 3443.279 kb on + strand, within BPHYT_RS15130 at 3443.279 kb on + strand, within BPHYT_RS15130 at 3443.546 kb on + strand, within BPHYT_RS15130 at 3443.547 kb on - strand, within BPHYT_RS15130 at 3443.547 kb on - strand, within BPHYT_RS15130 at 3443.657 kb on + strand at 3443.751 kb on - strand, within BPHYT_RS15130 at 3443.870 kb on + strand, within BPHYT_RS15130 at 3443.871 kb on - strand, within BPHYT_RS15130 at 3443.871 kb on - strand, within BPHYT_RS15130 at 3443.961 kb on - strand, within BPHYT_RS15130 at 3444.047 kb on + strand, within BPHYT_RS15130 at 3444.047 kb on + strand, within BPHYT_RS15130 at 3444.048 kb on - strand, within BPHYT_RS15130
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,440,844 + +3.3 3,440,845 - -0.5 3,440,845 - +1.4 3,441,198 + BPHYT_RS15115 0.80 +0.1 3,441,199 - BPHYT_RS15115 0.80 +0.4 3,441,448 + BPHYT_RS15120 0.24 -0.5 3,441,642 + BPHYT_RS15120 0.70 +1.8 3,441,752 + +0.7 3,441,794 - -0.0 3,441,864 + +1.1 3,441,999 + BPHYT_RS15125 0.15 +0.1 3,442,165 - BPHYT_RS15125 0.29 +0.2 3,442,165 - BPHYT_RS15125 0.29 +0.4 3,442,288 + BPHYT_RS15125 0.39 +1.1 3,442,288 + BPHYT_RS15125 0.39 +0.7 3,442,288 + BPHYT_RS15125 0.39 +0.0 3,442,381 - BPHYT_RS15125 0.46 +0.5 3,442,576 - BPHYT_RS15125 0.62 -0.0 3,442,650 + BPHYT_RS15125 0.68 +1.0 3,442,705 - BPHYT_RS15125 0.72 +0.2 3,442,760 + BPHYT_RS15125 0.77 -1.2 3,442,988 - +0.6 3,443,076 + -1.6 3,443,076 + -1.1 3,443,077 - +0.1 3,443,077 - -1.6 3,443,077 - -1.0 3,443,114 + +0.0 3,443,114 + -1.3 3,443,151 - +0.2 3,443,178 + BPHYT_RS15130 0.11 +0.9 3,443,179 - BPHYT_RS15130 0.11 -1.7 3,443,204 + BPHYT_RS15130 0.13 +0.9 3,443,279 + BPHYT_RS15130 0.19 +0.2 3,443,279 + BPHYT_RS15130 0.19 +2.1 3,443,546 + BPHYT_RS15130 0.41 -1.5 3,443,547 - BPHYT_RS15130 0.41 -0.5 3,443,547 - BPHYT_RS15130 0.41 -0.8 3,443,657 + +2.2 3,443,751 - BPHYT_RS15130 0.57 +0.3 3,443,870 + BPHYT_RS15130 0.67 -0.6 3,443,871 - BPHYT_RS15130 0.67 -2.5 3,443,871 - BPHYT_RS15130 0.67 -0.4 3,443,961 - BPHYT_RS15130 0.74 +1.1 3,444,047 + BPHYT_RS15130 0.81 +0.3 3,444,047 + BPHYT_RS15130 0.81 -0.9 3,444,048 - BPHYT_RS15130 0.82 +0.3
Or see this region's nucleotide sequence