Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15035

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15030 and BPHYT_RS15035 are separated by 304 nucleotidesBPHYT_RS15035 and BPHYT_RS15040 are separated by 92 nucleotides BPHYT_RS15030: BPHYT_RS15030 - acetyltransferase, at 3,418,575 to 3,419,099 _RS15030 BPHYT_RS15035: BPHYT_RS15035 - hypothetical protein, at 3,419,404 to 3,420,522 _RS15035 BPHYT_RS15040: BPHYT_RS15040 - hypothetical protein, at 3,420,615 to 3,421,346 _RS15040 Position (kb) 3419 3420 3421Strain fitness (log2 ratio) -2 -1 0 1 2at 3418.465 kb on + strandat 3418.465 kb on + strandat 3418.465 kb on + strandat 3418.465 kb on + strandat 3418.500 kb on + strandat 3418.501 kb on - strandat 3418.501 kb on - strandat 3418.511 kb on + strandat 3418.511 kb on + strandat 3418.515 kb on + strandat 3418.516 kb on - strandat 3418.565 kb on + strandat 3418.668 kb on + strand, within BPHYT_RS15030at 3418.728 kb on + strand, within BPHYT_RS15030at 3418.729 kb on - strand, within BPHYT_RS15030at 3418.774 kb on + strand, within BPHYT_RS15030at 3418.774 kb on + strand, within BPHYT_RS15030at 3418.882 kb on - strand, within BPHYT_RS15030at 3418.960 kb on - strand, within BPHYT_RS15030at 3418.965 kb on + strand, within BPHYT_RS15030at 3418.966 kb on - strand, within BPHYT_RS15030at 3418.966 kb on - strand, within BPHYT_RS15030at 3418.966 kb on - strand, within BPHYT_RS15030at 3419.001 kb on + strand, within BPHYT_RS15030at 3419.064 kb on - strandat 3419.064 kb on - strandat 3419.064 kb on - strandat 3419.082 kb on + strandat 3419.083 kb on - strandat 3419.204 kb on - strandat 3419.253 kb on + strandat 3419.254 kb on - strandat 3419.254 kb on - strandat 3419.383 kb on - strandat 3419.475 kb on - strandat 3419.549 kb on - strand, within BPHYT_RS15035at 3419.549 kb on - strand, within BPHYT_RS15035at 3419.862 kb on + strand, within BPHYT_RS15035at 3420.520 kb on + strandat 3420.521 kb on - strandat 3420.521 kb on - strandat 3420.539 kb on + strandat 3420.539 kb on + strandat 3420.540 kb on - strandat 3420.546 kb on - strandat 3420.546 kb on - strandat 3420.617 kb on - strandat 3420.847 kb on + strand, within BPHYT_RS15040at 3420.848 kb on - strand, within BPHYT_RS15040at 3420.848 kb on - strand, within BPHYT_RS15040at 3420.925 kb on + strand, within BPHYT_RS15040at 3420.926 kb on - strand, within BPHYT_RS15040at 3421.000 kb on + strand, within BPHYT_RS15040at 3421.004 kb on - strand, within BPHYT_RS15040at 3421.212 kb on - strand, within BPHYT_RS15040

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,418,465 + -1.3
3,418,465 + -0.2
3,418,465 + -0.6
3,418,465 + -0.3
3,418,500 + -1.4
3,418,501 - +1.9
3,418,501 - -1.6
3,418,511 + +1.0
3,418,511 + +0.1
3,418,515 + +0.0
3,418,516 - -0.0
3,418,565 + -0.2
3,418,668 + BPHYT_RS15030 0.18 +0.1
3,418,728 + BPHYT_RS15030 0.29 +0.4
3,418,729 - BPHYT_RS15030 0.29 -0.5
3,418,774 + BPHYT_RS15030 0.38 +0.0
3,418,774 + BPHYT_RS15030 0.38 -1.2
3,418,882 - BPHYT_RS15030 0.58 -0.6
3,418,960 - BPHYT_RS15030 0.73 -0.8
3,418,965 + BPHYT_RS15030 0.74 -0.4
3,418,966 - BPHYT_RS15030 0.74 +0.0
3,418,966 - BPHYT_RS15030 0.74 +0.2
3,418,966 - BPHYT_RS15030 0.74 -1.6
3,419,001 + BPHYT_RS15030 0.81 -0.6
3,419,064 - +0.0
3,419,064 - -1.2
3,419,064 - +1.2
3,419,082 + -2.1
3,419,083 - +0.5
3,419,204 - -0.3
3,419,253 + -0.7
3,419,254 - +0.9
3,419,254 - +1.4
3,419,383 - -0.6
3,419,475 - +0.2
3,419,549 - BPHYT_RS15035 0.13 -1.1
3,419,549 - BPHYT_RS15035 0.13 -0.7
3,419,862 + BPHYT_RS15035 0.41 +0.7
3,420,520 + -1.1
3,420,521 - +1.2
3,420,521 - +0.8
3,420,539 + +0.1
3,420,539 + +0.3
3,420,540 - +0.5
3,420,546 - +0.2
3,420,546 - -0.3
3,420,617 - -0.6
3,420,847 + BPHYT_RS15040 0.32 +0.5
3,420,848 - BPHYT_RS15040 0.32 +0.3
3,420,848 - BPHYT_RS15040 0.32 +1.0
3,420,925 + BPHYT_RS15040 0.42 +1.0
3,420,926 - BPHYT_RS15040 0.42 +1.6
3,421,000 + BPHYT_RS15040 0.53 -0.2
3,421,004 - BPHYT_RS15040 0.53 +0.3
3,421,212 - BPHYT_RS15040 0.82 +0.4

Or see this region's nucleotide sequence