Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14930 and BPHYT_RS14935 are separated by 534 nucleotides BPHYT_RS14935 and BPHYT_RS14940 are separated by 192 nucleotides BPHYT_RS14940 and BPHYT_RS14945 are separated by 31 nucleotides
BPHYT_RS14930: BPHYT_RS14930 - DNA gyrase subunit A, at 3,394,154 to 3,396,742
_RS14930
BPHYT_RS14935: BPHYT_RS14935 - membrane protein, at 3,397,277 to 3,397,924
_RS14935
BPHYT_RS14940: BPHYT_RS14940 - ubiquinone biosynthesis O-methyltransferase, at 3,398,117 to 3,398,815
_RS14940
BPHYT_RS14945: BPHYT_RS14945 - phosphoglycolate phosphatase, at 3,398,847 to 3,399,560
_RS14945
Position (kb)
3397
3398 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3396.825 kb on - strand at 3396.825 kb on - strand at 3396.884 kb on - strand at 3396.918 kb on + strand at 3396.918 kb on + strand at 3396.918 kb on + strand at 3396.918 kb on + strand at 3396.924 kb on + strand at 3397.163 kb on + strand at 3397.195 kb on + strand at 3397.922 kb on + strand at 3397.973 kb on + strand at 3398.057 kb on - strand at 3398.057 kb on - strand at 3398.058 kb on + strand at 3398.058 kb on + strand at 3398.058 kb on + strand at 3398.059 kb on - strand at 3398.060 kb on + strand at 3398.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,396,825 - -0.2 3,396,825 - -1.7 3,396,884 - -1.0 3,396,918 + -0.0 3,396,918 + -0.4 3,396,918 + -0.7 3,396,918 + -0.9 3,396,924 + +0.2 3,397,163 + -1.2 3,397,195 + +0.4 3,397,922 + +2.7 3,397,973 + +0.9 3,398,057 - +0.2 3,398,057 - +1.2 3,398,058 + +1.5 3,398,058 + -0.4 3,398,058 + -2.7 3,398,059 - +0.2 3,398,060 + -1.2 3,398,837 + -0.3
Or see this region's nucleotide sequence