Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14520

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
3,309,367	+		BPHYT_RS14515	0.38	-1.1
3,309,368	-		BPHYT_RS14515	0.38	-0.1
3,309,368	-		BPHYT_RS14515	0.38	-0.5
3,309,368	-		BPHYT_RS14515	0.38	-0.7
3,309,584	+		BPHYT_RS14515	0.53	-0.6
3,309,585	-		BPHYT_RS14515	0.53	+1.1
3,309,708	-		BPHYT_RS14515	0.61	-0.5
3,309,858	+		BPHYT_RS14515	0.71	-1.9
3,309,859	-		BPHYT_RS14515	0.71	+0.6
3,309,859	-		BPHYT_RS14515	0.71	-1.0
3,309,864	-		BPHYT_RS14515	0.72	+0.1
3,310,221	-				+0.3
3,310,261	+				+1.0
3,310,261	+				-0.2
3,310,261	+				-1.9
3,310,261	+				-0.5
3,310,261	+				-1.3
3,310,262	-				+2.7
3,310,262	-				-0.1
3,310,262	-				-0.6
3,310,515	-		BPHYT_RS14520	0.25	+0.7
3,310,635	+		BPHYT_RS14520	0.37	-0.8
3,310,636	-		BPHYT_RS14520	0.37	+0.6
3,310,636	-		BPHYT_RS14520	0.37	+0.1
3,310,643	+		BPHYT_RS14520	0.38	-0.3
3,310,644	-		BPHYT_RS14520	0.38	-0.1
3,310,688	+		BPHYT_RS14520	0.42	-0.6
3,310,689	-		BPHYT_RS14520	0.43	+0.3
3,310,689	-		BPHYT_RS14520	0.43	+0.4
3,310,692	+		BPHYT_RS14520	0.43	-0.6
3,310,692	+		BPHYT_RS14520	0.43	-2.0
3,310,693	-		BPHYT_RS14520	0.43	+2.2
3,310,693	-		BPHYT_RS14520	0.43	+0.9
3,310,747	-		BPHYT_RS14520	0.48	+0.3
3,310,747	-		BPHYT_RS14520	0.48	-1.3
3,310,747	-		BPHYT_RS14520	0.48	+0.5
3,310,860	+		BPHYT_RS14520	0.60	+0.0
3,310,860	+		BPHYT_RS14520	0.60	+0.2
3,310,860	+		BPHYT_RS14520	0.60	-1.4
3,310,861	-		BPHYT_RS14520	0.60	-0.3
3,311,034	+		BPHYT_RS14520	0.77	-0.9
3,311,035	-		BPHYT_RS14520	0.77	-0.5
3,311,048	-		BPHYT_RS14520	0.79	-0.1
3,311,197	-				+0.6
3,311,197	-				-1.1
3,311,271	-				-0.5
3,311,364	-				-0.6
3,311,442	-		BPHYT_RS14525	0.10	+0.0
3,311,442	-		BPHYT_RS14525	0.10	-1.3
3,311,442	-		BPHYT_RS14525	0.10	-0.7
3,311,442	-		BPHYT_RS14525	0.10	+0.3
3,311,442	-		BPHYT_RS14525	0.10	+2.4
3,311,442	-		BPHYT_RS14525	0.10	+2.4
3,311,552	+		BPHYT_RS14525	0.18	+0.7
3,311,552	+		BPHYT_RS14525	0.18	+0.7
3,311,552	+		BPHYT_RS14525	0.18	+2.2
3,311,553	-		BPHYT_RS14525	0.18	-1.1
3,311,603	+		BPHYT_RS14525	0.21	-0.9
3,311,793	+		BPHYT_RS14525	0.35	-0.4
3,311,794	-		BPHYT_RS14525	0.35	-0.7
3,312,050	+		BPHYT_RS14525	0.52	+0.4
3,312,051	-		BPHYT_RS14525	0.53	+1.0
3,312,051	-		BPHYT_RS14525	0.53	+1.5
3,312,051	-		BPHYT_RS14525	0.53	+0.7
3,312,204	+		BPHYT_RS14525	0.63	-1.5
3,312,217	+		BPHYT_RS14525	0.64	-0.2
3,312,218	-		BPHYT_RS14525	0.64	-2.1
3,312,218	-		BPHYT_RS14525	0.64	+0.7