Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14470 and BPHYT_RS14475 overlap by 4 nucleotides BPHYT_RS14475 and BPHYT_RS14480 overlap by 8 nucleotides BPHYT_RS14480 and BPHYT_RS14485 are separated by 729 nucleotides
BPHYT_RS14470: BPHYT_RS14470 - Rieske (2Fe-2S) protein, at 3,298,459 to 3,298,863
_RS14470
BPHYT_RS14475: BPHYT_RS14475 - HAD family hydrolase, at 3,298,860 to 3,299,519
_RS14475
BPHYT_RS14480: BPHYT_RS14480 - ribosomal large subunit pseudouridine synthase C, at 3,299,512 to 3,300,537
_RS14480
BPHYT_RS14485: BPHYT_RS14485 - ribonuclease E, at 3,301,267 to 3,304,560
_RS14485
Position (kb)
3299
3300
3301 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3298.602 kb on - strand, within BPHYT_RS14470 at 3298.618 kb on - strand, within BPHYT_RS14470 at 3298.618 kb on - strand, within BPHYT_RS14470 at 3298.631 kb on + strand, within BPHYT_RS14470 at 3298.631 kb on + strand, within BPHYT_RS14470 at 3298.632 kb on - strand, within BPHYT_RS14470 at 3298.684 kb on + strand, within BPHYT_RS14470 at 3298.712 kb on + strand, within BPHYT_RS14470 at 3298.727 kb on + strand, within BPHYT_RS14470 at 3298.917 kb on - strand at 3298.957 kb on + strand, within BPHYT_RS14475 at 3298.957 kb on + strand, within BPHYT_RS14475 at 3298.958 kb on - strand, within BPHYT_RS14475 at 3298.958 kb on - strand, within BPHYT_RS14475 at 3298.958 kb on - strand, within BPHYT_RS14475 at 3299.207 kb on - strand, within BPHYT_RS14475 at 3299.380 kb on + strand, within BPHYT_RS14475 at 3299.380 kb on + strand, within BPHYT_RS14475 at 3299.381 kb on - strand, within BPHYT_RS14475 at 3299.513 kb on + strand at 3299.514 kb on - strand at 3299.514 kb on - strand at 3299.551 kb on + strand at 3299.552 kb on - strand at 3299.552 kb on - strand at 3299.709 kb on + strand, within BPHYT_RS14480 at 3299.710 kb on - strand, within BPHYT_RS14480 at 3299.710 kb on - strand, within BPHYT_RS14480 at 3299.825 kb on + strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480 at 3299.826 kb on - strand, within BPHYT_RS14480 at 3299.919 kb on - strand, within BPHYT_RS14480 at 3299.919 kb on - strand, within BPHYT_RS14480 at 3299.990 kb on + strand, within BPHYT_RS14480 at 3299.990 kb on + strand, within BPHYT_RS14480 at 3299.990 kb on + strand, within BPHYT_RS14480 at 3299.991 kb on - strand, within BPHYT_RS14480 at 3299.991 kb on - strand, within BPHYT_RS14480 at 3299.991 kb on - strand, within BPHYT_RS14480 at 3299.991 kb on - strand, within BPHYT_RS14480 at 3300.201 kb on + strand, within BPHYT_RS14480 at 3300.235 kb on - strand, within BPHYT_RS14480 at 3300.339 kb on - strand, within BPHYT_RS14480 at 3300.400 kb on - strand, within BPHYT_RS14480 at 3300.400 kb on - strand, within BPHYT_RS14480 at 3300.400 kb on - strand, within BPHYT_RS14480 at 3300.518 kb on - strand at 3300.544 kb on + strand at 3301.179 kb on + strand at 3301.179 kb on + strand at 3301.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,298,602 - BPHYT_RS14470 0.35 -0.7 3,298,618 - BPHYT_RS14470 0.39 -0.5 3,298,618 - BPHYT_RS14470 0.39 -0.0 3,298,631 + BPHYT_RS14470 0.42 -0.6 3,298,631 + BPHYT_RS14470 0.42 +1.3 3,298,632 - BPHYT_RS14470 0.43 +0.1 3,298,684 + BPHYT_RS14470 0.56 +0.7 3,298,712 + BPHYT_RS14470 0.62 +0.8 3,298,727 + BPHYT_RS14470 0.66 +0.2 3,298,917 - +1.6 3,298,957 + BPHYT_RS14475 0.15 -1.2 3,298,957 + BPHYT_RS14475 0.15 -2.1 3,298,958 - BPHYT_RS14475 0.15 +0.8 3,298,958 - BPHYT_RS14475 0.15 +0.1 3,298,958 - BPHYT_RS14475 0.15 +0.8 3,299,207 - BPHYT_RS14475 0.53 +0.8 3,299,380 + BPHYT_RS14475 0.79 -2.1 3,299,380 + BPHYT_RS14475 0.79 +0.0 3,299,381 - BPHYT_RS14475 0.79 -0.1 3,299,513 + +0.5 3,299,514 - -0.4 3,299,514 - +1.4 3,299,551 + +0.1 3,299,552 - +1.1 3,299,552 - +0.3 3,299,709 + BPHYT_RS14480 0.19 -0.2 3,299,710 - BPHYT_RS14480 0.19 -1.3 3,299,710 - BPHYT_RS14480 0.19 -0.5 3,299,825 + BPHYT_RS14480 0.31 -0.1 3,299,826 - BPHYT_RS14480 0.31 -0.5 3,299,826 - BPHYT_RS14480 0.31 -0.6 3,299,826 - BPHYT_RS14480 0.31 -1.5 3,299,826 - BPHYT_RS14480 0.31 +0.1 3,299,919 - BPHYT_RS14480 0.40 +0.2 3,299,919 - BPHYT_RS14480 0.40 -1.9 3,299,990 + BPHYT_RS14480 0.47 -1.3 3,299,990 + BPHYT_RS14480 0.47 -1.8 3,299,990 + BPHYT_RS14480 0.47 +0.0 3,299,991 - BPHYT_RS14480 0.47 -0.5 3,299,991 - BPHYT_RS14480 0.47 -1.2 3,299,991 - BPHYT_RS14480 0.47 -0.4 3,299,991 - BPHYT_RS14480 0.47 -1.7 3,300,201 + BPHYT_RS14480 0.67 +0.4 3,300,235 - BPHYT_RS14480 0.70 -0.0 3,300,339 - BPHYT_RS14480 0.81 +0.4 3,300,400 - BPHYT_RS14480 0.87 -0.6 3,300,400 - BPHYT_RS14480 0.87 -0.5 3,300,400 - BPHYT_RS14480 0.87 -1.6 3,300,518 - -2.0 3,300,544 + -0.3 3,301,179 + -2.1 3,301,179 + -1.6 3,301,179 + -3.4
Or see this region's nucleotide sequence