Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14400 and BPHYT_RS14405 are separated by 83 nucleotides BPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotides BPHYT_RS14410 and BPHYT_RS14415 are separated by 117 nucleotides
BPHYT_RS14400: BPHYT_RS14400 - anti-sigma factor, at 3,286,835 to 3,287,464
_RS14400
BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147
_RS14405
BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632
_RS14410
BPHYT_RS14415: BPHYT_RS14415 - 3-oxoacyl-ACP synthase, at 3,288,750 to 3,289,988
_RS14415
Position (kb)
3288
3289 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.357 kb on - strand, within BPHYT_RS14400 at 3287.479 kb on + strand at 3287.479 kb on + strand at 3287.480 kb on - strand at 3287.480 kb on - strand at 3287.485 kb on + strand at 3287.485 kb on + strand at 3287.486 kb on - strand at 3287.514 kb on - strand at 3288.193 kb on - strand at 3288.193 kb on - strand at 3288.218 kb on - strand at 3288.218 kb on - strand at 3288.324 kb on + strand, within BPHYT_RS14410 at 3288.494 kb on - strand, within BPHYT_RS14410 at 3288.546 kb on - strand, within BPHYT_RS14410 at 3288.583 kb on + strand, within BPHYT_RS14410
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,287,314 - BPHYT_RS14400 0.76 -3.5 3,287,314 - BPHYT_RS14400 0.76 -3.1 3,287,357 - BPHYT_RS14400 0.83 -1.5 3,287,479 + -2.4 3,287,479 + -4.1 3,287,480 - -1.1 3,287,480 - -1.2 3,287,485 + -2.1 3,287,485 + -3.6 3,287,486 - +1.1 3,287,514 - -0.6 3,288,193 - -1.9 3,288,193 - +0.4 3,288,218 - -0.9 3,288,218 - -0.8 3,288,324 + BPHYT_RS14410 0.20 -1.2 3,288,494 - BPHYT_RS14410 0.64 +0.6 3,288,546 - BPHYT_RS14410 0.77 -0.7 3,288,583 + BPHYT_RS14410 0.87 +4.4
Or see this region's nucleotide sequence