Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14275

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14270 and BPHYT_RS14275 are separated by 18 nucleotidesBPHYT_RS14275 and BPHYT_RS14280 are separated by 17 nucleotidesBPHYT_RS14280 and BPHYT_RS14285 are separated by 60 nucleotides BPHYT_RS14270: BPHYT_RS14270 - electron transporter, at 3,261,905 to 3,262,519 _RS14270 BPHYT_RS14275: BPHYT_RS14275 - hypothetical protein, at 3,262,538 to 3,262,999 _RS14275 BPHYT_RS14280: BPHYT_RS14280 - membrane protein, at 3,263,017 to 3,263,886 _RS14280 BPHYT_RS14285: BPHYT_RS14285 - drug:proton antiporter, at 3,263,947 to 3,264,441 _RS14285 Position (kb) 3262 3263Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3261.578 kb on + strandat 3261.578 kb on + strandat 3261.579 kb on - strandat 3261.579 kb on - strandat 3261.579 kb on - strandat 3261.579 kb on - strandat 3261.774 kb on - strandat 3261.781 kb on + strandat 3261.782 kb on - strandat 3261.792 kb on + strandat 3261.792 kb on + strandat 3261.793 kb on - strandat 3261.793 kb on - strandat 3261.793 kb on - strandat 3261.793 kb on - strandat 3261.793 kb on - strandat 3261.793 kb on - strandat 3261.793 kb on - strandat 3262.113 kb on - strand, within BPHYT_RS14270at 3262.122 kb on - strand, within BPHYT_RS14270at 3262.265 kb on + strand, within BPHYT_RS14270at 3262.265 kb on + strand, within BPHYT_RS14270at 3262.265 kb on + strand, within BPHYT_RS14270at 3262.266 kb on - strand, within BPHYT_RS14270at 3262.266 kb on - strand, within BPHYT_RS14270at 3262.388 kb on + strand, within BPHYT_RS14270at 3262.389 kb on - strand, within BPHYT_RS14270at 3262.402 kb on + strand, within BPHYT_RS14270at 3262.402 kb on + strand, within BPHYT_RS14270at 3262.402 kb on + strand, within BPHYT_RS14270at 3262.415 kb on + strand, within BPHYT_RS14270at 3262.416 kb on - strand, within BPHYT_RS14270at 3262.755 kb on - strand, within BPHYT_RS14275at 3262.997 kb on + strandat 3262.998 kb on - strandat 3263.029 kb on + strandat 3263.171 kb on - strand, within BPHYT_RS14280at 3263.171 kb on - strand, within BPHYT_RS14280at 3263.171 kb on - strand, within BPHYT_RS14280at 3263.171 kb on - strand, within BPHYT_RS14280at 3263.198 kb on - strand, within BPHYT_RS14280at 3263.316 kb on + strand, within BPHYT_RS14280at 3263.317 kb on - strand, within BPHYT_RS14280at 3263.428 kb on + strand, within BPHYT_RS14280at 3263.672 kb on - strand, within BPHYT_RS14280at 3263.829 kb on + strandat 3263.890 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,261,578 + -1.2
3,261,578 + +0.8
3,261,579 - -0.4
3,261,579 - -0.3
3,261,579 - -0.0
3,261,579 - -0.1
3,261,774 - -3.1
3,261,781 + -0.5
3,261,782 - -5.8
3,261,792 + -1.4
3,261,792 + +0.7
3,261,793 - -4.4
3,261,793 - -1.4
3,261,793 - -1.1
3,261,793 - -1.7
3,261,793 - -3.2
3,261,793 - -3.8
3,261,793 - -1.9
3,262,113 - BPHYT_RS14270 0.34 -4.3
3,262,122 - BPHYT_RS14270 0.35 -2.6
3,262,265 + BPHYT_RS14270 0.59 +0.2
3,262,265 + BPHYT_RS14270 0.59 +1.1
3,262,265 + BPHYT_RS14270 0.59 -0.8
3,262,266 - BPHYT_RS14270 0.59 -3.4
3,262,266 - BPHYT_RS14270 0.59 -1.8
3,262,388 + BPHYT_RS14270 0.79 -2.3
3,262,389 - BPHYT_RS14270 0.79 -4.4
3,262,402 + BPHYT_RS14270 0.81 -1.3
3,262,402 + BPHYT_RS14270 0.81 -1.0
3,262,402 + BPHYT_RS14270 0.81 -0.5
3,262,415 + BPHYT_RS14270 0.83 -1.2
3,262,416 - BPHYT_RS14270 0.83 -6.0
3,262,755 - BPHYT_RS14275 0.47 -5.4
3,262,997 + +0.4
3,262,998 - -1.7
3,263,029 + -2.4
3,263,171 - BPHYT_RS14280 0.18 -0.4
3,263,171 - BPHYT_RS14280 0.18 +0.4
3,263,171 - BPHYT_RS14280 0.18 -1.2
3,263,171 - BPHYT_RS14280 0.18 +0.3
3,263,198 - BPHYT_RS14280 0.21 -0.4
3,263,316 + BPHYT_RS14280 0.34 -1.3
3,263,317 - BPHYT_RS14280 0.34 -0.5
3,263,428 + BPHYT_RS14280 0.47 +0.2
3,263,672 - BPHYT_RS14280 0.75 -0.8
3,263,829 + +0.9
3,263,890 + +0.7

Or see this region's nucleotide sequence