Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13975

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13970 and BPHYT_RS13975 are separated by 114 nucleotidesBPHYT_RS13975 and BPHYT_RS13980 are separated by 171 nucleotides BPHYT_RS13970: BPHYT_RS13970 - MFS transporter, at 3,190,174 to 3,191,439 _RS13970 BPHYT_RS13975: BPHYT_RS13975 - LysR family transcriptional regulator, at 3,191,554 to 3,192,471 _RS13975 BPHYT_RS13980: BPHYT_RS13980 - alanine--tRNA ligase, at 3,192,643 to 3,195,267 _RS13980 Position (kb) 3191 3192 3193Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 3190.701 kb on - strand, within BPHYT_RS13970at 3190.722 kb on - strand, within BPHYT_RS13970at 3190.980 kb on - strand, within BPHYT_RS13970at 3191.048 kb on - strand, within BPHYT_RS13970at 3191.480 kb on - strandat 3191.480 kb on - strandat 3191.488 kb on - strandat 3191.491 kb on - strandat 3191.721 kb on - strand, within BPHYT_RS13975at 3191.721 kb on - strand, within BPHYT_RS13975at 3191.753 kb on - strand, within BPHYT_RS13975at 3192.095 kb on + strand, within BPHYT_RS13975at 3192.155 kb on - strand, within BPHYT_RS13975at 3192.155 kb on - strand, within BPHYT_RS13975at 3192.307 kb on - strand, within BPHYT_RS13975at 3192.644 kb on + strandat 3192.644 kb on + strandat 3192.645 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,190,701 - BPHYT_RS13970 0.42 -0.1
3,190,722 - BPHYT_RS13970 0.43 -0.7
3,190,980 - BPHYT_RS13970 0.64 +1.6
3,191,048 - BPHYT_RS13970 0.69 -0.4
3,191,480 - -1.0
3,191,480 - +0.4
3,191,488 - -1.8
3,191,491 - -0.3
3,191,721 - BPHYT_RS13975 0.18 +5.6
3,191,721 - BPHYT_RS13975 0.18 +0.3
3,191,753 - BPHYT_RS13975 0.22 +0.5
3,192,095 + BPHYT_RS13975 0.59 +0.7
3,192,155 - BPHYT_RS13975 0.65 +0.1
3,192,155 - BPHYT_RS13975 0.65 +0.5
3,192,307 - BPHYT_RS13975 0.82 +0.4
3,192,644 + -1.8
3,192,644 + +0.9
3,192,645 - +1.1

Or see this region's nucleotide sequence