Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13600

Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13595 and BPHYT_RS13600 are separated by 44 nucleotidesBPHYT_RS13600 and BPHYT_RS13605 are separated by 741 nucleotidesBPHYT_RS13605 and BPHYT_RS13610 are separated by 63 nucleotides BPHYT_RS13595: BPHYT_RS13595 - IclR family transcriptional regulator, at 3,100,980 to 3,101,804 _RS13595 BPHYT_RS13600: BPHYT_RS13600 - thioesterase, at 3,101,849 to 3,102,421 _RS13600 BPHYT_RS13605: BPHYT_RS13605 - tRNA-Pro, at 3,103,163 to 3,103,239 _RS13605 BPHYT_RS13610: BPHYT_RS13610 - MerR family transcriptional regulator, at 3,103,303 to 3,103,716 _RS13610 Position (kb) 3101 3102 3103Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3100.884 kb on + strandat 3101.147 kb on + strand, within BPHYT_RS13595at 3101.176 kb on - strand, within BPHYT_RS13595at 3101.226 kb on + strand, within BPHYT_RS13595at 3101.265 kb on + strand, within BPHYT_RS13595at 3101.376 kb on + strand, within BPHYT_RS13595at 3101.417 kb on + strand, within BPHYT_RS13595at 3101.417 kb on + strand, within BPHYT_RS13595at 3101.469 kb on + strand, within BPHYT_RS13595at 3101.469 kb on + strand, within BPHYT_RS13595at 3101.542 kb on - strand, within BPHYT_RS13595at 3101.601 kb on + strand, within BPHYT_RS13595at 3101.774 kb on + strandat 3101.780 kb on + strandat 3101.781 kb on - strandat 3101.781 kb on - strandat 3101.834 kb on + strandat 3101.856 kb on - strandat 3102.306 kb on - strand, within BPHYT_RS13600at 3102.350 kb on - strand, within BPHYT_RS13600at 3102.390 kb on - strandat 3102.390 kb on - strandat 3102.474 kb on + strandat 3102.517 kb on + strandat 3102.517 kb on + strandat 3102.519 kb on + strandat 3102.529 kb on - strandat 3102.643 kb on + strandat 3102.761 kb on + strandat 3102.780 kb on + strandat 3102.780 kb on + strandat 3102.781 kb on - strandat 3102.837 kb on - strandat 3102.905 kb on + strandat 3102.921 kb on - strandat 3103.155 kb on - strandat 3103.214 kb on - strand, within BPHYT_RS13605at 3103.256 kb on - strandat 3103.256 kb on - strandat 3103.325 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
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3,100,884 + +1.3
3,101,147 + BPHYT_RS13595 0.20 -1.0
3,101,176 - BPHYT_RS13595 0.24 -1.0
3,101,226 + BPHYT_RS13595 0.30 -3.8
3,101,265 + BPHYT_RS13595 0.35 -4.1
3,101,376 + BPHYT_RS13595 0.48 -1.7
3,101,417 + BPHYT_RS13595 0.53 -3.4
3,101,417 + BPHYT_RS13595 0.53 -3.8
3,101,469 + BPHYT_RS13595 0.59 +0.2
3,101,469 + BPHYT_RS13595 0.59 -3.5
3,101,542 - BPHYT_RS13595 0.68 -3.4
3,101,601 + BPHYT_RS13595 0.75 -4.5
3,101,774 + +0.4
3,101,780 + -2.9
3,101,781 - -4.2
3,101,781 - -3.8
3,101,834 + -0.8
3,101,856 - +2.0
3,102,306 - BPHYT_RS13600 0.80 -0.1
3,102,350 - BPHYT_RS13600 0.87 +1.2
3,102,390 - -1.1
3,102,390 - -0.2
3,102,474 + +0.1
3,102,517 + -0.7
3,102,517 + -0.1
3,102,519 + +0.2
3,102,529 - +0.9
3,102,643 + -0.1
3,102,761 + +0.6
3,102,780 + -0.1
3,102,780 + -1.2
3,102,781 - -0.9
3,102,837 - +0.5
3,102,905 + -1.2
3,102,921 - +0.3
3,103,155 - -0.1
3,103,214 - BPHYT_RS13605 0.66 -2.9
3,103,256 - +0.9
3,103,256 - +0.2
3,103,325 - +2.4

Or see this region's nucleotide sequence