Experiment: Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13455 and BPHYT_RS13460 are separated by 22 nucleotides BPHYT_RS13460 and BPHYT_RS13465 are separated by 45 nucleotides BPHYT_RS13465 and BPHYT_RS13470 are separated by 94 nucleotides
BPHYT_RS13455: BPHYT_RS13455 - methyl-accepting chemotaxis protein, at 3,070,996 to 3,072,795
_RS13455
BPHYT_RS13460: BPHYT_RS13460 - chemotaxis protein, at 3,072,818 to 3,074,398
_RS13460
BPHYT_RS13465: BPHYT_RS13465 - hypothetical protein, at 3,074,444 to 3,074,824
_RS13465
BPHYT_RS13470: BPHYT_RS13470 - arylsulfate sulfotransferase, at 3,074,919 to 3,075,269
_RS13470
Position (kb)
3072
3073
3074
3075 Strain fitness (log2 ratio)
-1
0
1
2 at 3072.827 kb on + strand at 3072.828 kb on - strand at 3072.829 kb on + strand at 3073.220 kb on + strand, within BPHYT_RS13460 at 3073.399 kb on + strand, within BPHYT_RS13460 at 3074.153 kb on - strand, within BPHYT_RS13460 at 3074.423 kb on + strand at 3074.563 kb on - strand, within BPHYT_RS13465 at 3074.631 kb on + strand, within BPHYT_RS13465 at 3074.632 kb on - strand, within BPHYT_RS13465 at 3074.765 kb on - strand, within BPHYT_RS13465 at 3074.839 kb on + strand at 3075.022 kb on + strand, within BPHYT_RS13470 at 3075.257 kb on - strand at 3075.259 kb on - strand at 3075.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 remove 3,072,827 + -1.1 3,072,828 - -0.3 3,072,829 + +0.1 3,073,220 + BPHYT_RS13460 0.25 -0.9 3,073,399 + BPHYT_RS13460 0.37 +0.2 3,074,153 - BPHYT_RS13460 0.84 +0.9 3,074,423 + +0.4 3,074,563 - BPHYT_RS13465 0.31 +0.3 3,074,631 + BPHYT_RS13465 0.49 -0.8 3,074,632 - BPHYT_RS13465 0.49 -1.3 3,074,765 - BPHYT_RS13465 0.84 -1.1 3,074,839 + -0.2 3,075,022 + BPHYT_RS13470 0.29 -0.3 3,075,257 - +1.0 3,075,259 - +2.0 3,075,350 + +0.7
Or see this region's nucleotide sequence